Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3029 and GFF3030 are separated by 100 nucleotides GFF3030 and GFF3031 are separated by 204 nucleotides
GFF3029 - Putative metalloprotease yggG (EC 3.4.24.-), at 43,794 to 44,552
GFF3029
GFF3030 - Agmatinase (EC 3.5.3.11), at 44,653 to 45,573
GFF3030
GFF3031 - FIG01048344: hypothetical protein, at 45,778 to 46,668
GFF3031
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 43.678 kb on + strand at 43.679 kb on - strand at 43.679 kb on - strand at 43.721 kb on + strand at 43.722 kb on - strand at 43.811 kb on - strand at 43.865 kb on + strand at 43.865 kb on + strand at 43.866 kb on - strand at 43.907 kb on + strand, within GFF3029 at 43.908 kb on - strand, within GFF3029 at 43.999 kb on - strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.067 kb on - strand, within GFF3029 at 44.118 kb on - strand, within GFF3029 at 44.212 kb on + strand, within GFF3029 at 44.377 kb on - strand, within GFF3029 at 44.616 kb on + strand at 44.679 kb on + strand at 44.681 kb on + strand at 44.681 kb on + strand at 44.692 kb on + strand at 44.738 kb on + strand at 44.739 kb on - strand at 44.897 kb on + strand, within GFF3030 at 44.941 kb on - strand, within GFF3030 at 45.071 kb on + strand, within GFF3030 at 45.110 kb on + strand, within GFF3030 at 45.219 kb on + strand, within GFF3030 at 45.220 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030 at 45.667 kb on - strand at 45.707 kb on - strand at 45.711 kb on + strand at 45.760 kb on + strand at 45.779 kb on + strand at 45.827 kb on + strand at 45.925 kb on - strand, within GFF3031 at 45.970 kb on + strand, within GFF3031 at 45.970 kb on + strand, within GFF3031 at 46.105 kb on - strand, within GFF3031 at 46.205 kb on + strand, within GFF3031 at 46.205 kb on + strand, within GFF3031 at 46.258 kb on + strand, within GFF3031 at 46.387 kb on - strand, within GFF3031 at 46.387 kb on - strand, within GFF3031
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 43,678 + +0.4 43,679 - -0.3 43,679 - -0.2 43,721 + +0.8 43,722 - +0.2 43,811 - +0.5 43,865 + +0.5 43,865 + +0.7 43,866 - -0.1 43,907 + GFF3029 0.15 +0.1 43,908 - GFF3029 0.15 +0.0 43,999 - GFF3029 0.27 -0.1 44,064 + GFF3029 0.36 +0.0 44,064 + GFF3029 0.36 +0.8 44,065 - GFF3029 0.36 +1.0 44,065 - GFF3029 0.36 -0.2 44,067 - GFF3029 0.36 +0.1 44,118 - GFF3029 0.43 -0.3 44,212 + GFF3029 0.55 -1.1 44,377 - GFF3029 0.77 +0.2 44,616 + +1.4 44,679 + +1.0 44,681 + -0.6 44,681 + -1.0 44,692 + -0.3 44,738 + -0.5 44,739 - -2.3 44,897 + GFF3030 0.26 -1.6 44,941 - GFF3030 0.31 +0.0 45,071 + GFF3030 0.45 -0.3 45,110 + GFF3030 0.50 -0.3 45,219 + GFF3030 0.61 -1.1 45,220 - GFF3030 0.62 -0.8 45,297 - GFF3030 0.70 -1.3 45,297 - GFF3030 0.70 +0.4 45,667 - +0.0 45,707 - +0.4 45,711 + +0.3 45,760 + -0.1 45,779 + +0.2 45,827 + +0.1 45,925 - GFF3031 0.16 +0.6 45,970 + GFF3031 0.22 -0.3 45,970 + GFF3031 0.22 +0.6 46,105 - GFF3031 0.37 +1.4 46,205 + GFF3031 0.48 -0.0 46,205 + GFF3031 0.48 -0.4 46,258 + GFF3031 0.54 -0.8 46,387 - GFF3031 0.68 +0.3 46,387 - GFF3031 0.68 -0.0
Or see this region's nucleotide sequence