Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2884

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2882 and GFF2883 are separated by 4 nucleotidesGFF2883 and GFF2884 overlap by 23 nucleotidesGFF2884 and GFF2885 are separated by 32 nucleotides GFF2882 - Lipopolysaccharide biosynthesis protein RffA, at 6,397 to 7,527 GFF2882 GFF2883 - Lipopolysaccharide biosynthesis protein RffC, at 7,532 to 8,209 GFF2883 GFF2884 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 8,187 to 8,411 GFF2884 GFF2885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 8,444 to 9,511 GFF2885 Position (kb) 8 9Strain fitness (log2 ratio) -1 0 1at 7.209 kb on + strand, within GFF2882at 7.209 kb on + strand, within GFF2882at 7.290 kb on + strand, within GFF2882at 7.481 kb on + strandat 7.481 kb on + strandat 7.481 kb on + strandat 7.539 kb on + strandat 7.540 kb on - strandat 7.580 kb on + strandat 7.581 kb on - strandat 7.584 kb on + strandat 7.980 kb on - strand, within GFF2883at 8.168 kb on - strandat 8.180 kb on + strandat 8.237 kb on - strand, within GFF2884at 8.350 kb on - strand, within GFF2884at 8.350 kb on - strand, within GFF2884at 8.360 kb on + strand, within GFF2884at 8.431 kb on - strandat 8.615 kb on + strand, within GFF2885at 8.615 kb on + strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.616 kb on - strand, within GFF2885at 8.648 kb on - strand, within GFF2885at 8.766 kb on + strand, within GFF2885at 8.766 kb on + strand, within GFF2885at 8.766 kb on + strand, within GFF2885at 8.766 kb on + strand, within GFF2885at 8.766 kb on + strand, within GFF2885at 8.767 kb on - strand, within GFF2885at 8.767 kb on - strand, within GFF2885at 8.767 kb on - strand, within GFF2885at 8.802 kb on + strand, within GFF2885at 8.803 kb on - strand, within GFF2885at 8.862 kb on + strand, within GFF2885at 8.931 kb on + strand, within GFF2885at 8.945 kb on - strand, within GFF2885at 8.945 kb on - strand, within GFF2885at 8.945 kb on - strand, within GFF2885at 8.947 kb on + strand, within GFF2885at 8.947 kb on + strand, within GFF2885at 8.948 kb on - strand, within GFF2885at 8.969 kb on - strand, within GFF2885at 8.979 kb on + strand, within GFF2885at 8.984 kb on - strand, within GFF2885at 9.052 kb on - strand, within GFF2885at 9.118 kb on + strand, within GFF2885at 9.118 kb on + strand, within GFF2885at 9.195 kb on + strand, within GFF2885at 9.195 kb on + strand, within GFF2885at 9.195 kb on + strand, within GFF2885at 9.195 kb on + strand, within GFF2885at 9.196 kb on - strand, within GFF2885at 9.251 kb on + strand, within GFF2885at 9.252 kb on - strand, within GFF2885

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
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7,209 + GFF2882 0.72 -0.7
7,209 + GFF2882 0.72 -0.2
7,290 + GFF2882 0.79 +0.9
7,481 + -0.9
7,481 + -0.2
7,481 + -0.3
7,539 + +0.3
7,540 - +0.2
7,580 + -0.1
7,581 - -0.5
7,584 + -0.4
7,980 - GFF2883 0.66 +0.9
8,168 - +0.9
8,180 + +0.0
8,237 - GFF2884 0.22 +0.9
8,350 - GFF2884 0.72 +0.1
8,350 - GFF2884 0.72 +0.3
8,360 + GFF2884 0.77 -0.1
8,431 - +0.6
8,615 + GFF2885 0.16 +0.1
8,615 + GFF2885 0.16 -0.1
8,616 - GFF2885 0.16 +0.7
8,616 - GFF2885 0.16 -0.2
8,616 - GFF2885 0.16 +0.7
8,616 - GFF2885 0.16 +1.5
8,616 - GFF2885 0.16 +0.0
8,616 - GFF2885 0.16 +1.5
8,648 - GFF2885 0.19 -0.4
8,766 + GFF2885 0.30 -0.3
8,766 + GFF2885 0.30 -1.2
8,766 + GFF2885 0.30 +1.1
8,766 + GFF2885 0.30 +0.8
8,766 + GFF2885 0.30 +0.1
8,767 - GFF2885 0.30 -0.6
8,767 - GFF2885 0.30 -0.0
8,767 - GFF2885 0.30 -0.3
8,802 + GFF2885 0.34 +0.4
8,803 - GFF2885 0.34 -0.3
8,862 + GFF2885 0.39 +0.7
8,931 + GFF2885 0.46 +0.8
8,945 - GFF2885 0.47 -0.2
8,945 - GFF2885 0.47 +0.9
8,945 - GFF2885 0.47 +0.4
8,947 + GFF2885 0.47 -0.7
8,947 + GFF2885 0.47 +0.3
8,948 - GFF2885 0.47 -0.2
8,969 - GFF2885 0.49 +0.8
8,979 + GFF2885 0.50 -1.0
8,984 - GFF2885 0.51 -1.1
9,052 - GFF2885 0.57 -0.1
9,118 + GFF2885 0.63 +0.3
9,118 + GFF2885 0.63 -0.3
9,195 + GFF2885 0.70 +0.9
9,195 + GFF2885 0.70 +1.0
9,195 + GFF2885 0.70 -0.2
9,195 + GFF2885 0.70 +0.2
9,196 - GFF2885 0.70 +0.7
9,251 + GFF2885 0.76 -0.8
9,252 - GFF2885 0.76 +0.3

Or see this region's nucleotide sequence