Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2776

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2775 and GFF2776 are separated by 167 nucleotidesGFF2776 and GFF2777 are separated by 17 nucleotidesGFF2777 and GFF2778 overlap by 1 nucleotides GFF2775 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 299,723 to 300,979 GFF2775 GFF2776 - hypothetical protein, at 301,147 to 301,275 GFF2776 GFF2777 - Outer membrane lipoprotein LolB, at 301,293 to 301,916 GFF2777 GFF2778 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 301,916 to 302,764 GFF2778 Position (kb) 301 302Strain fitness (log2 ratio) -1 0 1at 301.049 kb on - strandat 301.157 kb on + strandat 301.260 kb on + strand, within GFF2776at 301.260 kb on + strand, within GFF2776at 301.260 kb on + strand, within GFF2776

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
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301,049 - -0.0
301,157 + +0.1
301,260 + GFF2776 0.88 -0.5
301,260 + GFF2776 0.88 -0.5
301,260 + GFF2776 0.88 -0.5

Or see this region's nucleotide sequence