Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF23

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF21 and GFF22 are separated by 7 nucleotidesGFF22 and GFF23 are separated by 38 nucleotidesGFF23 and GFF24 are separated by 391 nucleotidesGFF24 and GFF25 overlap by 37 nucleotides GFF21 - Methionine ABC transporter ATP-binding protein, at 1,035 to 2,066 GFF21 GFF22 - Methionine ABC transporter permease protein, at 2,074 to 2,712 GFF22 GFF23 - Methionine ABC transporter substrate-binding protein, at 2,751 to 3,566 GFF23 GFF24 - Protein RcsF, at 3,958 to 4,089 GFF24 GFF25 - COG1720: Uncharacterized conserved protein, at 4,053 to 4,793 GFF25 Position (kb) 2 3 4Strain fitness (log2 ratio) -1 0 1at 1.920 kb on - strand, within GFF21at 1.922 kb on + strand, within GFF21at 1.923 kb on - strand, within GFF21at 1.968 kb on - strandat 2.044 kb on + strandat 2.050 kb on + strandat 2.173 kb on + strand, within GFF22at 2.174 kb on - strand, within GFF22at 2.197 kb on - strand, within GFF22at 2.263 kb on - strand, within GFF22at 2.339 kb on + strand, within GFF22at 2.376 kb on + strand, within GFF22at 2.396 kb on + strand, within GFF22at 2.515 kb on - strand, within GFF22at 2.594 kb on - strand, within GFF22at 2.609 kb on - strand, within GFF22at 2.609 kb on - strand, within GFF22at 2.628 kb on + strand, within GFF22at 2.707 kb on - strandat 2.731 kb on + strandat 2.849 kb on + strand, within GFF23at 2.949 kb on - strand, within GFF23at 3.363 kb on + strand, within GFF23at 3.364 kb on - strand, within GFF23at 3.592 kb on - strandat 3.631 kb on - strandat 3.651 kb on - strandat 3.681 kb on + strandat 3.740 kb on + strandat 3.839 kb on - strandat 3.957 kb on + strandat 3.957 kb on + strandat 3.958 kb on - strandat 4.209 kb on + strand, within GFF25at 4.405 kb on + strand, within GFF25at 4.490 kb on + strand, within GFF25

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
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1,920 - GFF21 0.86 +0.4
1,922 + GFF21 0.86 -0.4
1,923 - GFF21 0.86 -0.5
1,968 - +0.0
2,044 + -0.2
2,050 + +0.5
2,173 + GFF22 0.15 +0.5
2,174 - GFF22 0.16 +0.3
2,197 - GFF22 0.19 -0.4
2,263 - GFF22 0.30 +0.3
2,339 + GFF22 0.41 +0.8
2,376 + GFF22 0.47 +0.6
2,396 + GFF22 0.50 -0.7
2,515 - GFF22 0.69 -0.9
2,594 - GFF22 0.81 -0.8
2,609 - GFF22 0.84 -0.2
2,609 - GFF22 0.84 -0.5
2,628 + GFF22 0.87 +0.1
2,707 - -1.7
2,731 + +0.4
2,849 + GFF23 0.12 -0.6
2,949 - GFF23 0.24 -0.4
3,363 + GFF23 0.75 -0.6
3,364 - GFF23 0.75 +0.0
3,592 - +0.4
3,631 - -1.0
3,651 - -0.2
3,681 + -1.6
3,740 + -0.4
3,839 - +1.2
3,957 + -0.0
3,957 + +0.1
3,958 - +1.0
4,209 + GFF25 0.21 -0.9
4,405 + GFF25 0.48 +0.3
4,490 + GFF25 0.59 -0.1

Or see this region's nucleotide sequence