Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2262 and GFF2263 are separated by 126 nucleotides GFF2263 and GFF2264 are separated by 142 nucleotides
GFF2262 - Lactaldehyde dehydrogenase involved in fucose or rhamnose utilization (EC 1.2.1.22), at 52,731 to 53,879
GFF2262
GFF2263 - Rhamnulose-1-phosphate aldolase (EC 4.1.2.19), at 54,006 to 54,833
GFF2263
GFF2264 - L-rhamnose isomerase (EC 5.3.1.14), at 54,976 to 56,235
GFF2264
Position (kb) 54
55 Strain fitness (log2 ratio) -1
0
1 at 53.178 kb on - strand, within GFF2262 at 53.217 kb on + strand, within GFF2262 at 53.275 kb on + strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.469 kb on - strand, within GFF2262 at 53.472 kb on - strand, within GFF2262 at 53.475 kb on - strand, within GFF2262 at 53.870 kb on + strand at 53.871 kb on - strand at 53.960 kb on + strand at 54.004 kb on + strand at 54.005 kb on - strand at 54.123 kb on + strand, within GFF2263 at 54.124 kb on - strand, within GFF2263 at 54.147 kb on - strand, within GFF2263 at 54.176 kb on + strand, within GFF2263 at 54.177 kb on - strand, within GFF2263 at 54.379 kb on + strand, within GFF2263 at 54.380 kb on - strand, within GFF2263 at 54.399 kb on - strand, within GFF2263 at 54.417 kb on - strand, within GFF2263 at 54.749 kb on + strand, within GFF2263 at 54.750 kb on - strand, within GFF2263 at 54.822 kb on - strand at 54.849 kb on + strand at 54.879 kb on - strand at 54.982 kb on - strand at 55.007 kb on + strand at 55.065 kb on - strand at 55.065 kb on - strand at 55.131 kb on - strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.400 kb on + strand, within GFF2264 at 55.502 kb on + strand, within GFF2264 at 55.527 kb on - strand, within GFF2264 at 55.600 kb on - strand, within GFF2264
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 53,178 - GFF2262 0.39 -0.8 53,217 + GFF2262 0.42 -0.0 53,275 + GFF2262 0.47 +0.8 53,469 - GFF2262 0.64 +0.1 53,469 - GFF2262 0.64 -0.3 53,469 - GFF2262 0.64 +0.9 53,472 - GFF2262 0.64 -0.6 53,475 - GFF2262 0.65 +0.1 53,870 + +0.7 53,871 - -0.0 53,960 + -0.4 54,004 + +0.6 54,005 - -0.2 54,123 + GFF2263 0.14 -0.2 54,124 - GFF2263 0.14 -0.3 54,147 - GFF2263 0.17 -0.3 54,176 + GFF2263 0.21 +0.2 54,177 - GFF2263 0.21 -1.1 54,379 + GFF2263 0.45 +0.5 54,380 - GFF2263 0.45 -0.6 54,399 - GFF2263 0.47 +1.1 54,417 - GFF2263 0.50 +0.5 54,749 + GFF2263 0.90 -0.1 54,750 - GFF2263 0.90 -0.7 54,822 - -0.2 54,849 + -0.3 54,879 - +0.4 54,982 - +0.6 55,007 + +0.4 55,065 - -0.1 55,065 - -0.4 55,131 - GFF2264 0.12 -0.3 55,400 + GFF2264 0.34 -0.0 55,400 + GFF2264 0.34 -0.1 55,400 + GFF2264 0.34 +0.2 55,502 + GFF2264 0.42 -0.1 55,527 - GFF2264 0.44 -0.0 55,600 - GFF2264 0.50 +1.3
Or see this region's nucleotide sequence