Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1924 and GFF1925 are separated by 90 nucleotides GFF1925 and GFF1926 are separated by 210 nucleotides
GFF1924 - Putative amino acid transporter, at 116,143 to 117,564
GFF1924
GFF1925 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 117,655 to 119,052
GFF1925
GFF1926 - Arginine utilization regulatory protein RocR, at 119,263 to 120,690
GFF1926
Position (kb)
117
118
119
120 Strain fitness (log2 ratio)
-2
-1
0
1 at 116.697 kb on + strand, within GFF1924 at 116.698 kb on - strand, within GFF1924 at 116.710 kb on - strand, within GFF1924 at 116.757 kb on + strand, within GFF1924 at 116.757 kb on + strand, within GFF1924 at 116.758 kb on - strand, within GFF1924 at 116.758 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 116.835 kb on - strand, within GFF1924 at 117.013 kb on - strand, within GFF1924 at 117.018 kb on - strand, within GFF1924 at 117.088 kb on - strand, within GFF1924 at 117.088 kb on - strand, within GFF1924 at 117.321 kb on + strand, within GFF1924 at 117.322 kb on - strand, within GFF1924 at 117.542 kb on - strand at 117.608 kb on - strand at 117.608 kb on - strand at 117.654 kb on - strand at 117.762 kb on + strand at 117.763 kb on - strand at 117.953 kb on + strand, within GFF1925 at 117.954 kb on - strand, within GFF1925 at 117.994 kb on + strand, within GFF1925 at 118.093 kb on - strand, within GFF1925 at 118.142 kb on - strand, within GFF1925 at 118.217 kb on + strand, within GFF1925 at 118.218 kb on - strand, within GFF1925 at 118.218 kb on - strand, within GFF1925 at 118.222 kb on - strand, within GFF1925 at 118.225 kb on - strand, within GFF1925 at 118.404 kb on + strand, within GFF1925 at 118.405 kb on - strand, within GFF1925 at 118.596 kb on + strand, within GFF1925 at 118.629 kb on + strand, within GFF1925 at 118.629 kb on + strand, within GFF1925 at 118.630 kb on - strand, within GFF1925 at 118.656 kb on - strand, within GFF1925 at 118.878 kb on + strand, within GFF1925 at 118.908 kb on - strand, within GFF1925 at 119.018 kb on - strand at 119.018 kb on - strand at 119.226 kb on - strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.262 kb on + strand at 119.263 kb on - strand at 119.263 kb on - strand at 119.350 kb on - strand at 119.374 kb on + strand at 119.375 kb on - strand at 119.375 kb on - strand at 119.406 kb on + strand, within GFF1926 at 119.406 kb on + strand, within GFF1926 at 119.407 kb on - strand, within GFF1926 at 119.433 kb on + strand, within GFF1926 at 119.433 kb on + strand, within GFF1926 at 119.472 kb on + strand, within GFF1926 at 119.628 kb on + strand, within GFF1926 at 119.629 kb on - strand, within GFF1926 at 119.775 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.901 kb on + strand, within GFF1926 at 119.916 kb on + strand, within GFF1926
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 116,697 + GFF1924 0.39 -0.3 116,698 - GFF1924 0.39 -0.3 116,710 - GFF1924 0.40 +0.2 116,757 + GFF1924 0.43 -0.2 116,757 + GFF1924 0.43 +0.2 116,758 - GFF1924 0.43 +1.1 116,758 - GFF1924 0.43 -0.1 116,835 - GFF1924 0.49 -0.0 116,835 - GFF1924 0.49 -0.4 116,835 - GFF1924 0.49 +0.3 116,835 - GFF1924 0.49 +0.2 116,835 - GFF1924 0.49 -0.4 116,835 - GFF1924 0.49 -0.3 116,835 - GFF1924 0.49 +0.0 117,013 - GFF1924 0.61 +0.1 117,018 - GFF1924 0.62 -1.4 117,088 - GFF1924 0.66 +0.4 117,088 - GFF1924 0.66 +0.6 117,321 + GFF1924 0.83 -0.5 117,322 - GFF1924 0.83 +0.3 117,542 - +0.3 117,608 - +0.5 117,608 - -0.5 117,654 - -0.5 117,762 + +0.4 117,763 - +0.0 117,953 + GFF1925 0.21 +0.4 117,954 - GFF1925 0.21 -0.6 117,994 + GFF1925 0.24 -0.1 118,093 - GFF1925 0.31 -0.1 118,142 - GFF1925 0.35 +0.7 118,217 + GFF1925 0.40 +1.4 118,218 - GFF1925 0.40 -0.4 118,218 - GFF1925 0.40 +0.0 118,222 - GFF1925 0.41 +0.8 118,225 - GFF1925 0.41 +1.1 118,404 + GFF1925 0.54 +0.0 118,405 - GFF1925 0.54 -0.3 118,596 + GFF1925 0.67 +0.0 118,629 + GFF1925 0.70 +1.1 118,629 + GFF1925 0.70 +0.1 118,630 - GFF1925 0.70 +0.1 118,656 - GFF1925 0.72 +0.3 118,878 + GFF1925 0.87 +0.2 118,908 - GFF1925 0.90 +1.6 119,018 - -0.5 119,018 - -0.3 119,226 - +1.2 119,260 + -0.7 119,260 + -0.6 119,260 + +0.1 119,260 + -0.2 119,262 + -0.3 119,263 - +0.4 119,263 - -0.1 119,350 - +0.8 119,374 + -0.2 119,375 - +1.3 119,375 - +1.2 119,406 + GFF1926 0.10 +0.3 119,406 + GFF1926 0.10 +0.7 119,407 - GFF1926 0.10 -0.1 119,433 + GFF1926 0.12 -0.0 119,433 + GFF1926 0.12 -0.1 119,472 + GFF1926 0.15 -0.4 119,628 + GFF1926 0.26 +0.0 119,629 - GFF1926 0.26 -2.6 119,775 + GFF1926 0.36 +1.3 119,837 + GFF1926 0.40 +0.5 119,837 + GFF1926 0.40 +1.3 119,837 + GFF1926 0.40 -0.2 119,901 + GFF1926 0.45 -0.8 119,916 + GFF1926 0.46 +0.0
Or see this region's nucleotide sequence