Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1645

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF1643 and GFF1644 are separated by 1 nucleotidesGFF1644 and GFF1645 are separated by 36 nucleotidesGFF1645 and GFF1646 are separated by 29 nucleotidesGFF1646 and GFF1647 are separated by 102 nucleotides GFF1643 - hypothetical protein, at 29,048 to 29,647 GFF1643 GFF1644 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 29,649 to 30,170 GFF1644 GFF1645 - FIG000859: hypothetical protein YebC, at 30,207 to 30,947 GFF1645 GFF1646 - Dihydroneopterin triphosphate pyrophosphohydrolase type 2 (nudB), at 30,977 to 31,429 GFF1646 GFF1647 - Aspartyl-tRNA synthetase (EC 6.1.1.12), at 31,532 to 33,304 GFF1647 Position (kb) 30 31Strain fitness (log2 ratio) -1 0 1at 29.249 kb on - strand, within GFF1643at 29.378 kb on + strand, within GFF1643at 29.420 kb on - strand, within GFF1643at 29.423 kb on + strand, within GFF1643at 29.526 kb on + strand, within GFF1643at 29.549 kb on - strand, within GFF1643at 30.455 kb on - strand, within GFF1645at 30.455 kb on - strand, within GFF1645at 30.657 kb on - strand, within GFF1645at 30.689 kb on - strand, within GFF1645at 30.689 kb on - strand, within GFF1645at 30.933 kb on - strandat 30.933 kb on - strandat 30.936 kb on - strandat 31.089 kb on - strand, within GFF1646at 31.520 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
remove
29,249 - GFF1643 0.34 +0.9
29,378 + GFF1643 0.55 +0.2
29,420 - GFF1643 0.62 +0.0
29,423 + GFF1643 0.62 -0.2
29,526 + GFF1643 0.80 -0.4
29,549 - GFF1643 0.83 +0.7
30,455 - GFF1645 0.33 -0.1
30,455 - GFF1645 0.33 -0.4
30,657 - GFF1645 0.61 -0.5
30,689 - GFF1645 0.65 -0.4
30,689 - GFF1645 0.65 -0.0
30,933 - +0.7
30,933 - -0.8
30,936 - -0.6
31,089 - GFF1646 0.25 -0.4
31,520 - -0.7

Or see this region's nucleotide sequence