Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1536

Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1535 and GFF1536 are separated by 25 nucleotidesGFF1536 and GFF1537 are separated by 97 nucleotides GFF1535 - 2-dehydro-3-deoxyglucarate aldolase (EC 4.1.2.20), at 40,419 to 41,189 GFF1535 GFF1536 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 41,215 to 42,105 GFF1536 GFF1537 - Glycerate kinase (EC 2.7.1.31), at 42,203 to 43,348 GFF1537 Position (kb) 41 42 43Strain fitness (log2 ratio) -1 0 1at 40.245 kb on + strandat 40.360 kb on + strandat 40.430 kb on + strandat 40.592 kb on - strand, within GFF1535at 40.809 kb on - strand, within GFF1535at 40.862 kb on + strand, within GFF1535at 40.863 kb on - strand, within GFF1535at 40.863 kb on - strand, within GFF1535at 41.022 kb on - strand, within GFF1535at 41.137 kb on + strandat 41.243 kb on + strandat 41.244 kb on - strandat 41.244 kb on - strandat 41.416 kb on - strand, within GFF1536at 41.416 kb on - strand, within GFF1536at 41.458 kb on - strand, within GFF1536at 41.496 kb on + strand, within GFF1536at 41.497 kb on - strand, within GFF1536at 41.656 kb on + strand, within GFF1536at 41.657 kb on - strand, within GFF1536at 41.660 kb on - strand, within GFF1536at 41.931 kb on + strand, within GFF1536at 41.932 kb on - strand, within GFF1536at 41.956 kb on - strand, within GFF1536at 42.067 kb on + strandat 42.197 kb on - strandat 42.230 kb on + strandat 42.233 kb on - strandat 42.308 kb on + strandat 42.308 kb on + strandat 42.309 kb on - strandat 42.526 kb on + strand, within GFF1537at 42.788 kb on - strand, within GFF1537at 42.888 kb on - strand, within GFF1537at 42.996 kb on + strand, within GFF1537at 43.090 kb on + strand, within GFF1537

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
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40,245 + -0.2
40,360 + +1.5
40,430 + +0.8
40,592 - GFF1535 0.22 +0.1
40,809 - GFF1535 0.51 +0.3
40,862 + GFF1535 0.57 -0.2
40,863 - GFF1535 0.58 -0.2
40,863 - GFF1535 0.58 +0.0
41,022 - GFF1535 0.78 -0.3
41,137 + -0.2
41,243 + +0.3
41,244 - +1.2
41,244 - -1.7
41,416 - GFF1536 0.23 -0.3
41,416 - GFF1536 0.23 +0.2
41,458 - GFF1536 0.27 -0.4
41,496 + GFF1536 0.32 -1.2
41,497 - GFF1536 0.32 -0.5
41,656 + GFF1536 0.49 -0.7
41,657 - GFF1536 0.50 +0.2
41,660 - GFF1536 0.50 +0.0
41,931 + GFF1536 0.80 -0.3
41,932 - GFF1536 0.80 -0.2
41,956 - GFF1536 0.83 +0.1
42,067 + -0.2
42,197 - +1.0
42,230 + -0.2
42,233 - -0.1
42,308 + +0.3
42,308 + +0.0
42,309 - -0.0
42,526 + GFF1537 0.28 -0.3
42,788 - GFF1537 0.51 -0.1
42,888 - GFF1537 0.60 +0.1
42,996 + GFF1537 0.69 +0.5
43,090 + GFF1537 0.77 +0.4

Or see this region's nucleotide sequence