Experiment: LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1311 and GFF1312 are separated by 12 nucleotides GFF1312 and GFF1313 are separated by 521 nucleotides GFF1313 and GFF1314 are separated by 35 nucleotides
GFF1311 - FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated, at 18,717 to 20,963
GFF1311
GFF1312 - 'Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-)' transl_table=11, at 20,976 to 21,506
GFF1312
GFF1313 - hypothetical protein, at 22,028 to 22,153
GFF1313
GFF1314 - DNA mismatch repair endonuclease MutH, at 22,189 to 22,884
GFF1314
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.071 kb on - strand, within GFF1311 at 20.231 kb on - strand, within GFF1311 at 20.231 kb on - strand, within GFF1311 at 20.351 kb on + strand, within GFF1311 at 20.352 kb on - strand, within GFF1311 at 20.672 kb on - strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.813 kb on - strand at 20.813 kb on - strand at 20.861 kb on + strand at 20.940 kb on + strand at 20.963 kb on + strand at 21.027 kb on - strand at 21.090 kb on - strand, within GFF1312 at 21.156 kb on - strand, within GFF1312 at 21.195 kb on + strand, within GFF1312 at 21.328 kb on - strand, within GFF1312 at 21.465 kb on + strand at 21.474 kb on + strand at 21.474 kb on + strand at 21.475 kb on - strand at 21.512 kb on + strand at 21.523 kb on - strand at 21.529 kb on + strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.533 kb on + strand at 21.534 kb on - strand at 21.534 kb on - strand at 21.550 kb on + strand at 21.582 kb on - strand at 21.802 kb on + strand at 21.803 kb on - strand at 21.946 kb on + strand at 21.947 kb on - strand at 22.003 kb on + strand at 22.003 kb on + strand at 22.008 kb on + strand at 22.009 kb on - strand at 22.020 kb on + strand at 22.151 kb on + strand at 22.187 kb on + strand at 22.241 kb on + strand at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.269 kb on + strand, within GFF1314 at 22.270 kb on - strand, within GFF1314 at 22.270 kb on - strand, within GFF1314 at 22.416 kb on - strand, within GFF1314
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP30_phage 0.0000875 MOI remove 20,071 - GFF1311 0.60 +0.8 20,231 - GFF1311 0.67 +0.1 20,231 - GFF1311 0.67 -0.2 20,351 + GFF1311 0.73 -0.2 20,352 - GFF1311 0.73 -0.3 20,672 - GFF1311 0.87 +0.4 20,675 + GFF1311 0.87 +0.6 20,675 + GFF1311 0.87 +0.6 20,675 + GFF1311 0.87 +1.2 20,813 - +1.8 20,813 - -0.1 20,861 + +0.5 20,940 + +1.0 20,963 + +0.4 21,027 - -0.5 21,090 - GFF1312 0.21 -1.5 21,156 - GFF1312 0.34 -1.6 21,195 + GFF1312 0.41 -2.5 21,328 - GFF1312 0.66 -1.0 21,465 + -1.5 21,474 + -1.1 21,474 + -0.6 21,475 - -2.4 21,512 + -1.1 21,523 - +0.2 21,529 + -0.0 21,530 - +0.1 21,530 - -0.9 21,530 - +0.2 21,530 - +0.2 21,532 - +0.1 21,532 - +0.3 21,532 - -0.3 21,533 + -0.6 21,534 - -0.4 21,534 - +0.1 21,550 + -1.0 21,582 - -0.3 21,802 + +0.2 21,803 - -0.2 21,946 + -0.3 21,947 - +0.8 22,003 + +0.7 22,003 + +0.7 22,008 + +0.1 22,009 - +0.6 22,020 + +1.1 22,151 + -0.0 22,187 + +0.6 22,241 + -0.9 22,269 + GFF1314 0.11 -0.8 22,269 + GFF1314 0.11 -0.1 22,269 + GFF1314 0.11 -0.2 22,269 + GFF1314 0.11 +0.1 22,269 + GFF1314 0.11 -0.1 22,270 - GFF1314 0.12 +0.5 22,270 - GFF1314 0.12 -0.4 22,416 - GFF1314 0.33 -0.2
Or see this region's nucleotide sequence