Strain Fitness in Escherichia coli BL21 around ECD_00032

Experiment: Mixed culture, Bdellovibrio bacteriovorus Tiebrius_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00030 and ECD_00031 overlap by 1 nucleotidesECD_00031 and ECD_00032 are separated by 124 nucleotidesECD_00032 and ECD_00033 are separated by 1 nucleotides ECD_00030: ECD_00030 - isoleucyl-tRNA synthetase, at 26,463 to 29,279 _00030 ECD_00031: ECD_00031 - prolipoprotein signal peptidase (signal peptidase II), at 29,279 to 29,773 _00031 ECD_00032: ECD_00032 - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase), at 29,898 to 30,347 _00032 ECD_00033: ECD_00033 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein, at 30,349 to 31,299 _00033 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1at 29.755 kb on + strandat 29.771 kb on + strandat 29.774 kb on + strandat 29.774 kb on + strandat 29.853 kb on + strandat 29.881 kb on + strandat 30.138 kb on + strand, within ECD_00032at 30.138 kb on + strand, within ECD_00032at 31.319 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus Tiebrius_10
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29,755 + -0.3
29,771 + -0.0
29,774 + +1.3
29,774 + +1.2
29,853 + +0.3
29,881 + -0.3
30,138 + ECD_00032 0.53 +0.8
30,138 + ECD_00032 0.53 +0.8
31,319 - -2.2

Or see this region's nucleotide sequence