Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5532 and Atu5533 are separated by 1 nucleotides Atu5533 and Atu5534 are separated by 1 nucleotides Atu5534 and Atu5535 overlap by 1 nucleotides
Atu5532: Atu5532 - ABC transporter membrane spanning protein, at 526,472 to 527,281
Atu5532
Atu5533: Atu5533 - ABC transporter membrane spanning protein, at 527,283 to 528,422
Atu5533
Atu5534: Atu5534 - ABC transporter nucleotide binding/ATPase, at 528,424 to 529,173
Atu5534
Atu5535: Atu5535 - ABC transporter nucleotide binding/ATPase, at 529,173 to 529,868
Atu5535
Position (kb) 527
528
529 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 526.285 kb on - strand at 526.402 kb on + strand at 526.416 kb on + strand at 526.422 kb on - strand at 526.493 kb on - strand at 526.531 kb on + strand at 526.569 kb on + strand, within Atu5532 at 526.670 kb on + strand, within Atu5532 at 526.707 kb on + strand, within Atu5532 at 526.709 kb on - strand, within Atu5532 at 526.713 kb on + strand, within Atu5532 at 526.713 kb on + strand, within Atu5532 at 526.715 kb on - strand, within Atu5532 at 526.782 kb on + strand, within Atu5532 at 526.830 kb on - strand, within Atu5532 at 526.916 kb on - strand, within Atu5532 at 526.925 kb on + strand, within Atu5532 at 526.974 kb on + strand, within Atu5532 at 527.017 kb on - strand, within Atu5532 at 527.142 kb on - strand, within Atu5532 at 527.143 kb on - strand, within Atu5532 at 527.260 kb on + strand at 527.273 kb on - strand at 527.273 kb on - strand at 527.419 kb on + strand, within Atu5533 at 527.424 kb on + strand, within Atu5533 at 527.514 kb on - strand, within Atu5533 at 527.528 kb on - strand, within Atu5533 at 527.676 kb on - strand, within Atu5533 at 527.682 kb on - strand, within Atu5533 at 527.767 kb on + strand, within Atu5533 at 527.785 kb on - strand, within Atu5533 at 527.808 kb on - strand, within Atu5533 at 527.832 kb on + strand, within Atu5533 at 527.893 kb on + strand, within Atu5533 at 527.901 kb on - strand, within Atu5533 at 527.908 kb on + strand, within Atu5533 at 527.930 kb on + strand, within Atu5533 at 527.999 kb on - strand, within Atu5533 at 527.999 kb on - strand, within Atu5533 at 527.999 kb on - strand, within Atu5533 at 528.024 kb on - strand, within Atu5533 at 528.047 kb on - strand, within Atu5533 at 528.094 kb on - strand, within Atu5533 at 528.100 kb on + strand, within Atu5533 at 528.163 kb on - strand, within Atu5533 at 528.163 kb on - strand, within Atu5533 at 528.165 kb on + strand, within Atu5533 at 528.210 kb on + strand, within Atu5533 at 528.210 kb on + strand, within Atu5533 at 528.218 kb on - strand, within Atu5533 at 528.218 kb on - strand, within Atu5533 at 528.237 kb on - strand, within Atu5533 at 528.263 kb on + strand, within Atu5533 at 528.345 kb on - strand at 528.368 kb on + strand at 528.531 kb on + strand, within Atu5534 at 528.600 kb on + strand, within Atu5534 at 528.608 kb on - strand, within Atu5534 at 528.722 kb on + strand, within Atu5534 at 528.754 kb on - strand, within Atu5534 at 528.754 kb on - strand, within Atu5534 at 528.819 kb on - strand, within Atu5534 at 528.874 kb on - strand, within Atu5534 at 528.884 kb on + strand, within Atu5534 at 528.902 kb on - strand, within Atu5534 at 528.939 kb on + strand, within Atu5534 at 528.977 kb on - strand, within Atu5534 at 528.977 kb on - strand, within Atu5534 at 528.979 kb on + strand, within Atu5534 at 528.987 kb on - strand, within Atu5534 at 529.018 kb on - strand, within Atu5534 at 529.043 kb on - strand, within Atu5534 at 529.098 kb on - strand, within Atu5534 at 529.098 kb on - strand, within Atu5534 at 529.117 kb on + strand at 529.125 kb on - strand at 529.156 kb on - strand at 529.204 kb on - strand at 529.204 kb on - strand at 529.228 kb on + strand at 529.228 kb on + strand at 529.228 kb on + strand at 529.236 kb on - strand at 529.236 kb on - strand at 529.236 kb on - strand at 529.301 kb on + strand, within Atu5535 at 529.301 kb on + strand, within Atu5535 at 529.309 kb on + strand, within Atu5535 at 529.364 kb on - strand, within Atu5535 at 529.397 kb on + strand, within Atu5535 at 529.401 kb on + strand, within Atu5535 at 529.416 kb on + strand, within Atu5535 at 529.421 kb on + strand, within Atu5535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 526,285 - +0.4 526,402 + +2.8 526,416 + +0.7 526,422 - +3.0 526,493 - -0.6 526,531 + +0.3 526,569 + Atu5532 0.12 -0.1 526,670 + Atu5532 0.24 -1.2 526,707 + Atu5532 0.29 -0.5 526,709 - Atu5532 0.29 +0.2 526,713 + Atu5532 0.30 -1.9 526,713 + Atu5532 0.30 +1.3 526,715 - Atu5532 0.30 +0.7 526,782 + Atu5532 0.38 -0.9 526,830 - Atu5532 0.44 +0.4 526,916 - Atu5532 0.55 +0.7 526,925 + Atu5532 0.56 -0.2 526,974 + Atu5532 0.62 +0.5 527,017 - Atu5532 0.67 -0.5 527,142 - Atu5532 0.83 +0.1 527,143 - Atu5532 0.83 -1.8 527,260 + +1.0 527,273 - -0.1 527,273 - -0.8 527,419 + Atu5533 0.12 -0.1 527,424 + Atu5533 0.12 +0.3 527,514 - Atu5533 0.20 +0.1 527,528 - Atu5533 0.21 -0.2 527,676 - Atu5533 0.34 +1.3 527,682 - Atu5533 0.35 +0.3 527,767 + Atu5533 0.42 +2.9 527,785 - Atu5533 0.44 +2.6 527,808 - Atu5533 0.46 +0.1 527,832 + Atu5533 0.48 +0.6 527,893 + Atu5533 0.54 +1.3 527,901 - Atu5533 0.54 -1.1 527,908 + Atu5533 0.55 +1.4 527,930 + Atu5533 0.57 +0.5 527,999 - Atu5533 0.63 +0.2 527,999 - Atu5533 0.63 +0.1 527,999 - Atu5533 0.63 +1.2 528,024 - Atu5533 0.65 +0.1 528,047 - Atu5533 0.67 +2.9 528,094 - Atu5533 0.71 +0.6 528,100 + Atu5533 0.72 -0.3 528,163 - Atu5533 0.77 +1.0 528,163 - Atu5533 0.77 -0.0 528,165 + Atu5533 0.77 +0.9 528,210 + Atu5533 0.81 +0.5 528,210 + Atu5533 0.81 +1.3 528,218 - Atu5533 0.82 +0.1 528,218 - Atu5533 0.82 +0.5 528,237 - Atu5533 0.84 +1.4 528,263 + Atu5533 0.86 -0.2 528,345 - -0.1 528,368 + -1.2 528,531 + Atu5534 0.14 +0.3 528,600 + Atu5534 0.23 +0.7 528,608 - Atu5534 0.25 -0.5 528,722 + Atu5534 0.40 -0.2 528,754 - Atu5534 0.44 +0.1 528,754 - Atu5534 0.44 +0.8 528,819 - Atu5534 0.53 +0.7 528,874 - Atu5534 0.60 +0.0 528,884 + Atu5534 0.61 -0.2 528,902 - Atu5534 0.64 +0.4 528,939 + Atu5534 0.69 -0.8 528,977 - Atu5534 0.74 +0.4 528,977 - Atu5534 0.74 +0.1 528,979 + Atu5534 0.74 +0.5 528,987 - Atu5534 0.75 -0.0 529,018 - Atu5534 0.79 -0.7 529,043 - Atu5534 0.83 -0.4 529,098 - Atu5534 0.90 +0.3 529,098 - Atu5534 0.90 +0.2 529,117 + +0.5 529,125 - +0.1 529,156 - +0.5 529,204 - -1.3 529,204 - -0.6 529,228 + +0.5 529,228 + +1.5 529,228 + +0.3 529,236 - +0.2 529,236 - -0.1 529,236 - +0.6 529,301 + Atu5535 0.18 -0.1 529,301 + Atu5535 0.18 +0.2 529,309 + Atu5535 0.20 -0.5 529,364 - Atu5535 0.27 +0.4 529,397 + Atu5535 0.32 +1.6 529,401 + Atu5535 0.33 +0.2 529,416 + Atu5535 0.35 -0.1 529,421 + Atu5535 0.36 -1.6
Or see this region's nucleotide sequence