Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5052 and Atu5053 are separated by 72 nucleotides Atu5053 and Atu5054 are separated by 9 nucleotides Atu5054 and Atu5055 are separated by 11 nucleotides
Atu5052: Atu5052 - small heat shock protein, at 51,311 to 51,787
Atu5052
Atu5053: Atu5053 - hypothetical protein, at 51,860 to 52,159
Atu5053
Atu5054: Atu5054 - hypothetical protein, at 52,169 to 52,444
Atu5054
Atu5055: Atu5055 - ATP-dependent DNA ligase, at 52,456 to 55,110
Atu5055
Position (kb)
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.241 kb on + strand at 51.244 kb on - strand at 51.249 kb on - strand at 51.249 kb on - strand at 51.249 kb on - strand at 51.285 kb on - strand at 51.299 kb on - strand at 51.301 kb on + strand at 51.301 kb on + strand at 51.301 kb on + strand at 51.301 kb on + strand at 51.309 kb on - strand at 51.309 kb on - strand at 51.309 kb on - strand at 51.309 kb on - strand at 51.320 kb on + strand at 51.330 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.335 kb on + strand at 51.338 kb on - strand at 51.343 kb on - strand at 51.343 kb on - strand at 51.343 kb on - strand at 51.343 kb on - strand at 51.343 kb on - strand at 51.390 kb on - strand, within Atu5052 at 51.430 kb on - strand, within Atu5052 at 51.430 kb on - strand, within Atu5052 at 51.430 kb on - strand, within Atu5052 at 51.430 kb on - strand, within Atu5052 at 51.443 kb on + strand, within Atu5052 at 51.550 kb on - strand, within Atu5052 at 51.556 kb on + strand, within Atu5052 at 51.594 kb on + strand, within Atu5052 at 51.657 kb on + strand, within Atu5052 at 51.657 kb on - strand, within Atu5052 at 51.737 kb on - strand, within Atu5052 at 51.753 kb on + strand at 51.753 kb on + strand at 51.753 kb on + strand at 51.753 kb on + strand at 51.758 kb on + strand at 51.761 kb on - strand at 51.761 kb on - strand at 51.855 kb on + strand at 51.858 kb on - strand at 51.882 kb on - strand at 52.142 kb on + strand at 52.255 kb on - strand, within Atu5054 at 52.278 kb on + strand, within Atu5054 at 52.285 kb on - strand, within Atu5054 at 52.318 kb on + strand, within Atu5054 at 52.399 kb on + strand, within Atu5054 at 52.399 kb on + strand, within Atu5054 at 52.426 kb on - strand at 52.437 kb on - strand at 52.437 kb on - strand at 52.461 kb on - strand at 52.505 kb on + strand at 52.531 kb on - strand at 52.558 kb on + strand at 52.611 kb on + strand at 52.619 kb on - strand at 52.638 kb on - strand at 52.679 kb on - strand at 52.732 kb on + strand, within Atu5055 at 52.782 kb on - strand, within Atu5055 at 52.805 kb on + strand, within Atu5055 at 52.812 kb on + strand, within Atu5055 at 52.854 kb on + strand, within Atu5055 at 52.932 kb on + strand, within Atu5055 at 52.951 kb on - strand, within Atu5055 at 52.955 kb on + strand, within Atu5055 at 52.963 kb on - strand, within Atu5055 at 52.987 kb on + strand, within Atu5055 at 53.041 kb on - strand, within Atu5055 at 53.059 kb on + strand, within Atu5055 at 53.059 kb on + strand, within Atu5055 at 53.133 kb on - strand, within Atu5055 at 53.134 kb on - strand, within Atu5055 at 53.134 kb on - strand, within Atu5055 at 53.145 kb on + strand, within Atu5055 at 53.145 kb on + strand, within Atu5055 at 53.231 kb on + strand, within Atu5055 at 53.239 kb on - strand, within Atu5055 at 53.250 kb on + strand, within Atu5055 at 53.258 kb on - strand, within Atu5055 at 53.265 kb on + strand, within Atu5055 at 53.265 kb on + strand, within Atu5055 at 53.273 kb on - strand, within Atu5055 at 53.293 kb on + strand, within Atu5055 at 53.363 kb on + strand, within Atu5055 at 53.370 kb on - strand, within Atu5055 at 53.403 kb on + strand, within Atu5055 at 53.411 kb on - strand, within Atu5055 at 53.432 kb on - strand, within Atu5055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 51,241 + -0.1 51,244 - +0.6 51,249 - -0.4 51,249 - +0.4 51,249 - -0.4 51,285 - -0.5 51,299 - -0.2 51,301 + +0.3 51,301 + -1.0 51,301 + +0.0 51,301 + +0.2 51,309 - -0.2 51,309 - +0.3 51,309 - -0.5 51,309 - -0.4 51,320 + +0.1 51,330 + +1.1 51,335 + +0.6 51,335 + +2.1 51,335 + +0.4 51,335 + -1.6 51,335 + -0.6 51,335 + -0.2 51,335 + +0.1 51,335 + +0.1 51,338 - +0.1 51,343 - -1.2 51,343 - +0.6 51,343 - +0.2 51,343 - -0.4 51,343 - -1.9 51,390 - Atu5052 0.17 -0.1 51,430 - Atu5052 0.25 +0.8 51,430 - Atu5052 0.25 +0.0 51,430 - Atu5052 0.25 +0.3 51,430 - Atu5052 0.25 +0.3 51,443 + Atu5052 0.28 +1.2 51,550 - Atu5052 0.50 +0.2 51,556 + Atu5052 0.51 -0.5 51,594 + Atu5052 0.59 -0.2 51,657 + Atu5052 0.73 -0.4 51,657 - Atu5052 0.73 +0.6 51,737 - Atu5052 0.89 -0.2 51,753 + +0.3 51,753 + +0.5 51,753 + +1.1 51,753 + +0.1 51,758 + +0.2 51,761 - -0.9 51,761 - -0.1 51,855 + -1.3 51,858 - +2.0 51,882 - -0.3 52,142 + +0.2 52,255 - Atu5054 0.31 +0.3 52,278 + Atu5054 0.39 -0.2 52,285 - Atu5054 0.42 -0.5 52,318 + Atu5054 0.54 -0.8 52,399 + Atu5054 0.83 -1.6 52,399 + Atu5054 0.83 -1.4 52,426 - +0.6 52,437 - -0.2 52,437 - -0.3 52,461 - -0.5 52,505 + +0.1 52,531 - +0.4 52,558 + +0.5 52,611 + -0.4 52,619 - +0.2 52,638 - +1.0 52,679 - +0.1 52,732 + Atu5055 0.10 -0.1 52,782 - Atu5055 0.12 -1.0 52,805 + Atu5055 0.13 -0.5 52,812 + Atu5055 0.13 -0.3 52,854 + Atu5055 0.15 +0.2 52,932 + Atu5055 0.18 +0.1 52,951 - Atu5055 0.19 +0.3 52,955 + Atu5055 0.19 +0.3 52,963 - Atu5055 0.19 +0.1 52,987 + Atu5055 0.20 +2.4 53,041 - Atu5055 0.22 +1.0 53,059 + Atu5055 0.23 +0.7 53,059 + Atu5055 0.23 -0.6 53,133 - Atu5055 0.25 -1.4 53,134 - Atu5055 0.26 +0.1 53,134 - Atu5055 0.26 +0.3 53,145 + Atu5055 0.26 +0.6 53,145 + Atu5055 0.26 -0.1 53,231 + Atu5055 0.29 -0.1 53,239 - Atu5055 0.29 +1.3 53,250 + Atu5055 0.30 -0.3 53,258 - Atu5055 0.30 +0.8 53,265 + Atu5055 0.30 +1.3 53,265 + Atu5055 0.30 -0.3 53,273 - Atu5055 0.31 -0.3 53,293 + Atu5055 0.32 +0.3 53,363 + Atu5055 0.34 -0.0 53,370 - Atu5055 0.34 -0.4 53,403 + Atu5055 0.36 +0.1 53,411 - Atu5055 0.36 +1.1 53,432 - Atu5055 0.37 -0.6
Or see this region's nucleotide sequence