Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3288 and Atu3289 overlap by 14 nucleotides Atu3289 and Atu3290 overlap by 4 nucleotides Atu3290 and Atu3291 are separated by 216 nucleotides Atu3291 and Atu3292 are separated by 127 nucleotides
Atu3288: Atu3288 - hydroxyethylthiazole kinase, at 314,731 to 315,471
Atu3288
Atu3289: Atu3289 - thiamine-phosphate pyrophosphorylase, at 315,458 to 316,120
Atu3289
Atu3290: Atu3290 - phosphomethylpyrimidine kinase, at 316,117 to 316,914
Atu3290
Atu3291: Atu3291 - Lpr family transcriptional regulator, at 317,131 to 317,589
Atu3291
Atu3292: Atu3292 - alanine racemase, at 317,717 to 318,883
Atu3292
Position (kb) 316
317 Strain fitness (log2 ratio) -1
0
1 at 315.244 kb on + strand, within Atu3288 at 315.254 kb on + strand, within Atu3288 at 315.278 kb on + strand, within Atu3288 at 315.323 kb on + strand, within Atu3288 at 315.344 kb on + strand, within Atu3288 at 315.345 kb on - strand, within Atu3288 at 315.352 kb on - strand, within Atu3288 at 315.366 kb on + strand, within Atu3288 at 315.467 kb on + strand at 315.475 kb on - strand at 315.503 kb on - strand at 315.670 kb on - strand, within Atu3289 at 315.793 kb on - strand, within Atu3289 at 315.817 kb on + strand, within Atu3289 at 315.925 kb on + strand, within Atu3289 at 316.129 kb on - strand at 316.183 kb on + strand at 316.193 kb on + strand at 316.370 kb on - strand, within Atu3290 at 316.412 kb on - strand, within Atu3290 at 316.598 kb on + strand, within Atu3290 at 316.613 kb on - strand, within Atu3290 at 316.632 kb on + strand, within Atu3290 at 316.632 kb on + strand, within Atu3290 at 316.632 kb on + strand, within Atu3290 at 316.640 kb on - strand, within Atu3290 at 316.640 kb on - strand, within Atu3290 at 316.662 kb on - strand, within Atu3290 at 316.667 kb on + strand, within Atu3290 at 316.688 kb on + strand, within Atu3290 at 316.795 kb on - strand, within Atu3290 at 316.843 kb on - strand at 316.913 kb on + strand at 316.937 kb on - strand at 316.945 kb on + strand at 316.972 kb on + strand at 317.093 kb on - strand at 317.106 kb on + strand at 317.419 kb on + strand, within Atu3291 at 317.427 kb on - strand, within Atu3291 at 317.429 kb on + strand, within Atu3291 at 317.432 kb on - strand, within Atu3291 at 317.432 kb on - strand, within Atu3291 at 317.432 kb on - strand, within Atu3291 at 317.459 kb on + strand, within Atu3291 at 317.514 kb on + strand, within Atu3291 at 317.548 kb on - strand at 317.548 kb on - strand at 317.862 kb on + strand, within Atu3292
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 315,244 + Atu3288 0.69 +0.6 315,254 + Atu3288 0.71 +1.2 315,278 + Atu3288 0.74 -0.9 315,323 + Atu3288 0.80 -0.4 315,344 + Atu3288 0.83 -0.6 315,345 - Atu3288 0.83 -0.0 315,352 - Atu3288 0.84 -0.1 315,366 + Atu3288 0.86 +0.2 315,467 + +0.5 315,475 - +1.2 315,503 - +0.4 315,670 - Atu3289 0.32 +0.2 315,793 - Atu3289 0.51 -0.3 315,817 + Atu3289 0.54 +0.4 315,925 + Atu3289 0.70 -0.2 316,129 - +0.8 316,183 + -0.5 316,193 + +0.0 316,370 - Atu3290 0.32 +0.3 316,412 - Atu3290 0.37 -0.1 316,598 + Atu3290 0.60 +0.3 316,613 - Atu3290 0.62 -0.2 316,632 + Atu3290 0.65 +0.1 316,632 + Atu3290 0.65 +1.1 316,632 + Atu3290 0.65 -0.3 316,640 - Atu3290 0.66 -1.4 316,640 - Atu3290 0.66 +1.3 316,662 - Atu3290 0.68 -0.2 316,667 + Atu3290 0.69 +0.5 316,688 + Atu3290 0.72 +0.0 316,795 - Atu3290 0.85 -0.7 316,843 - +1.0 316,913 + -0.1 316,937 - -0.8 316,945 + +0.6 316,972 + -0.8 317,093 - -0.0 317,106 + +0.3 317,419 + Atu3291 0.63 -0.4 317,427 - Atu3291 0.64 -1.0 317,429 + Atu3291 0.65 +0.0 317,432 - Atu3291 0.66 +1.0 317,432 - Atu3291 0.66 -0.7 317,432 - Atu3291 0.66 -0.4 317,459 + Atu3291 0.71 -1.3 317,514 + Atu3291 0.83 +1.0 317,548 - -0.4 317,548 - +0.4 317,862 + Atu3292 0.12 +0.1
Or see this region's nucleotide sequence