Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Atu3230 and Atu8184 are separated by 157 nucleotides Atu8184 and Atu3232 overlap by 4 nucleotides Atu3232 and Atu8185 are separated by 104 nucleotides Atu8185 and Atu3234 are separated by 111 nucleotides 
        Atu3230: Atu3230 - hypothetical protein, at 247,037 to 247,801 
        Atu3230 
         
        
        Atu8184: Atu8184 - hypothetical protein, at 247,959 to 248,372 
        Atu8184 
         
        
        Atu3232: Atu3232 - hydroxypyruvate reductase, at 248,369 to 249,649 
        Atu3232 
         
        
        Atu8185: Atu8185 - hypothetical protein, at 249,754 to 250,017 
        Atu8185 
         
        
        Atu3234: Atu3234 - NAD binding oxidoreductase, at 250,129 to 251,316 
        Atu3234 
         Position (kb) 248 
249 
250 Strain fitness (log2 ratio) -1 
0 
1 
2 at 247.426 kb on - strand, within Atu3230 at 247.485 kb on + strand, within Atu3230 at 247.528 kb on - strand, within Atu3230 at 247.533 kb on - strand, within Atu3230 at 247.544 kb on + strand, within Atu3230 at 247.552 kb on - strand, within Atu3230 at 247.637 kb on - strand, within Atu3230 at 247.645 kb on + strand, within Atu3230 at 247.731 kb on - strand at 247.742 kb on - strand at 247.870 kb on + strand at 247.899 kb on - strand at 248.010 kb on - strand, within Atu8184 at 248.180 kb on - strand, within Atu8184 at 248.212 kb on + strand, within Atu8184 at 248.240 kb on - strand, within Atu8184 at 248.266 kb on - strand, within Atu8184 at 248.342 kb on - strand at 248.392 kb on - strand at 248.487 kb on + strand at 248.583 kb on + strand, within Atu3232 at 248.791 kb on - strand, within Atu3232 at 248.881 kb on + strand, within Atu3232 at 248.881 kb on + strand, within Atu3232 at 248.889 kb on - strand, within Atu3232 at 248.925 kb on - strand, within Atu3232 at 248.964 kb on + strand, within Atu3232 at 249.045 kb on - strand, within Atu3232 at 249.082 kb on + strand, within Atu3232 at 249.164 kb on + strand, within Atu3232 at 249.289 kb on + strand, within Atu3232 at 249.310 kb on + strand, within Atu3232 at 249.398 kb on - strand, within Atu3232 at 249.457 kb on - strand, within Atu3232 at 249.552 kb on - strand at 249.693 kb on + strand at 249.781 kb on - strand, within Atu8185 at 249.781 kb on - strand, within Atu8185 at 249.781 kb on - strand, within Atu8185 at 249.813 kb on - strand, within Atu8185 at 250.083 kb on - strand at 250.201 kb on - strand at 250.229 kb on + strand at 250.229 kb on + strand at 250.230 kb on + strand at 250.283 kb on + strand, within Atu3234 at 250.291 kb on - strand, within Atu3234 at 250.291 kb on - strand, within Atu3234 at 250.520 kb on - strand, within Atu3234 at 250.522 kb on + strand, within Atu3234 at 250.612 kb on + strand, within Atu3234 at 250.620 kb on - strand, within Atu3234  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 247,426 -  Atu3230 0.51  +2.2 247,485 +  Atu3230 0.59  +0.3 247,528 -  Atu3230 0.64  -1.0 247,533 -  Atu3230 0.65  +0.3 247,544 +  Atu3230 0.66  -0.6 247,552 -  Atu3230 0.67  +0.2 247,637 -  Atu3230 0.78  +0.8 247,645 +  Atu3230 0.79  -0.7 247,731 -  +0.1 247,742 -  -0.2 247,870 +  -0.2 247,899 -  +0.2 248,010 -  Atu8184 0.12  +1.3 248,180 -  Atu8184 0.53  -0.7 248,212 +  Atu8184 0.61  -0.8 248,240 -  Atu8184 0.68  -0.1 248,266 -  Atu8184 0.74  -0.1 248,342 -  -0.2 248,392 -  +1.1 248,487 +  +0.4 248,583 +  Atu3232 0.17  +0.2 248,791 -  Atu3232 0.33  +0.2 248,881 +  Atu3232 0.40  +0.1 248,881 +  Atu3232 0.40  +1.3 248,889 -  Atu3232 0.41  +0.3 248,925 -  Atu3232 0.43  +0.7 248,964 +  Atu3232 0.46  -0.0 249,045 -  Atu3232 0.53  +0.2 249,082 +  Atu3232 0.56  +2.2 249,164 +  Atu3232 0.62  +0.7 249,289 +  Atu3232 0.72  +0.3 249,310 +  Atu3232 0.73  +0.7 249,398 -  Atu3232 0.80  +0.7 249,457 -  Atu3232 0.85  +0.8 249,552 -  +0.2 249,693 +  +0.4 249,781 -  Atu8185 0.10  -0.2 249,781 -  Atu8185 0.10  +1.3 249,781 -  Atu8185 0.10  +0.3 249,813 -  Atu8185 0.22  -0.9 250,083 -  -0.0 250,201 -  +0.0 250,229 +  +0.1 250,229 +  -0.3 250,230 +  -0.0 250,283 +  Atu3234 0.13  -0.1 250,291 -  Atu3234 0.14  -0.4 250,291 -  Atu3234 0.14  +0.2 250,520 -  Atu3234 0.33  +0.1 250,522 +  Atu3234 0.33  +0.1 250,612 +  Atu3234 0.41  +0.6 250,620 -  Atu3234 0.41  +0.4 
Or see this region's nucleotide sequence