Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3085 and Atu3086 are separated by 11 nucleotides Atu3086 and Atu3087 are separated by 3 nucleotides Atu3087 and Atu3088 are separated by 237 nucleotides
Atu3085: Atu3085 - hydantoin racemase, at 90,839 to 91,582
Atu3085
Atu3086: Atu3086 - spermidine/putrescine ABC transporter permease, at 91,594 to 92,412
Atu3086
Atu3087: Atu3087 - spermidine/putrescine ABC transporter permease, at 92,416 to 93,327
Atu3087
Atu3088: Atu3088 - spermidine/putrescine ABC transporter substrate-binding protein, at 93,565 to 94,854
Atu3088
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 91.554 kb on + strand at 91.623 kb on - strand at 91.640 kb on - strand at 91.758 kb on - strand, within Atu3086 at 91.785 kb on + strand, within Atu3086 at 91.785 kb on - strand, within Atu3086 at 91.797 kb on + strand, within Atu3086 at 91.830 kb on - strand, within Atu3086 at 91.865 kb on - strand, within Atu3086 at 91.922 kb on + strand, within Atu3086 at 92.015 kb on - strand, within Atu3086 at 92.130 kb on - strand, within Atu3086 at 92.252 kb on - strand, within Atu3086 at 92.262 kb on - strand, within Atu3086 at 92.332 kb on - strand at 92.355 kb on - strand at 92.414 kb on + strand at 92.414 kb on + strand at 92.422 kb on - strand at 92.422 kb on - strand at 92.431 kb on + strand at 92.439 kb on - strand at 92.469 kb on - strand at 92.479 kb on + strand at 92.484 kb on + strand at 92.484 kb on + strand at 92.581 kb on - strand, within Atu3087 at 92.598 kb on - strand, within Atu3087 at 92.676 kb on + strand, within Atu3087 at 92.762 kb on + strand, within Atu3087 at 92.762 kb on + strand, within Atu3087 at 92.770 kb on - strand, within Atu3087 at 92.774 kb on + strand, within Atu3087 at 92.791 kb on + strand, within Atu3087 at 92.799 kb on - strand, within Atu3087 at 92.813 kb on + strand, within Atu3087 at 92.867 kb on + strand, within Atu3087 at 92.875 kb on - strand, within Atu3087 at 92.953 kb on - strand, within Atu3087 at 92.964 kb on - strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.020 kb on + strand, within Atu3087 at 93.028 kb on - strand, within Atu3087 at 93.083 kb on - strand, within Atu3087 at 93.090 kb on + strand, within Atu3087 at 93.090 kb on + strand, within Atu3087 at 93.115 kb on + strand, within Atu3087 at 93.139 kb on + strand, within Atu3087 at 93.244 kb on + strand at 93.272 kb on - strand at 93.312 kb on + strand at 93.312 kb on + strand at 93.332 kb on - strand at 93.376 kb on + strand at 93.444 kb on - strand at 93.484 kb on - strand at 93.500 kb on + strand at 93.508 kb on - strand at 93.516 kb on - strand at 93.599 kb on + strand at 93.662 kb on - strand at 93.737 kb on - strand, within Atu3088 at 93.742 kb on - strand, within Atu3088 at 93.757 kb on - strand, within Atu3088 at 93.790 kb on + strand, within Atu3088 at 93.848 kb on - strand, within Atu3088 at 93.875 kb on + strand, within Atu3088 at 93.898 kb on - strand, within Atu3088 at 93.909 kb on + strand, within Atu3088 at 93.910 kb on + strand, within Atu3088 at 93.917 kb on - strand, within Atu3088 at 93.918 kb on - strand, within Atu3088 at 93.931 kb on + strand, within Atu3088 at 94.071 kb on - strand, within Atu3088 at 94.081 kb on - strand, within Atu3088 at 94.097 kb on + strand, within Atu3088 at 94.296 kb on + strand, within Atu3088
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 91,554 + +1.0 91,623 - -0.5 91,640 - -0.5 91,758 - Atu3086 0.20 -0.1 91,785 + Atu3086 0.23 -0.7 91,785 - Atu3086 0.23 +0.2 91,797 + Atu3086 0.25 -0.4 91,830 - Atu3086 0.29 +0.5 91,865 - Atu3086 0.33 +0.2 91,922 + Atu3086 0.40 +0.2 92,015 - Atu3086 0.51 -0.3 92,130 - Atu3086 0.65 +0.9 92,252 - Atu3086 0.80 +0.8 92,262 - Atu3086 0.82 +0.0 92,332 - +0.4 92,355 - -2.4 92,414 + +0.2 92,414 + +0.0 92,422 - +1.0 92,422 - -0.1 92,431 + +2.0 92,439 - +0.0 92,469 - -0.3 92,479 + +0.0 92,484 + -0.1 92,484 + +0.3 92,581 - Atu3087 0.18 -0.3 92,598 - Atu3087 0.20 -0.4 92,676 + Atu3087 0.29 -0.2 92,762 + Atu3087 0.38 -0.2 92,762 + Atu3087 0.38 +1.0 92,770 - Atu3087 0.39 -0.5 92,774 + Atu3087 0.39 +0.1 92,791 + Atu3087 0.41 -0.1 92,799 - Atu3087 0.42 +0.5 92,813 + Atu3087 0.44 -0.6 92,867 + Atu3087 0.49 -1.0 92,875 - Atu3087 0.50 -0.6 92,953 - Atu3087 0.59 +0.1 92,964 - Atu3087 0.60 -0.8 93,020 + Atu3087 0.66 +0.5 93,020 + Atu3087 0.66 +0.7 93,020 + Atu3087 0.66 -0.7 93,020 + Atu3087 0.66 +0.7 93,020 + Atu3087 0.66 -0.3 93,020 + Atu3087 0.66 -1.5 93,020 + Atu3087 0.66 -2.1 93,028 - Atu3087 0.67 -2.0 93,083 - Atu3087 0.73 -0.2 93,090 + Atu3087 0.74 -0.3 93,090 + Atu3087 0.74 -0.7 93,115 + Atu3087 0.77 -0.5 93,139 + Atu3087 0.79 -0.2 93,244 + -1.5 93,272 - -0.3 93,312 + -0.1 93,312 + +0.1 93,332 - +0.7 93,376 + -1.2 93,444 - -0.3 93,484 - -0.4 93,500 + +0.6 93,508 - +0.6 93,516 - +0.3 93,599 + -0.2 93,662 - -0.4 93,737 - Atu3088 0.13 -0.8 93,742 - Atu3088 0.14 -0.4 93,757 - Atu3088 0.15 -0.3 93,790 + Atu3088 0.17 +2.7 93,848 - Atu3088 0.22 -0.5 93,875 + Atu3088 0.24 +0.5 93,898 - Atu3088 0.26 -0.4 93,909 + Atu3088 0.27 -0.3 93,910 + Atu3088 0.27 +0.3 93,917 - Atu3088 0.27 +0.6 93,918 - Atu3088 0.27 -0.4 93,931 + Atu3088 0.28 -0.8 94,071 - Atu3088 0.39 -0.2 94,081 - Atu3088 0.40 +0.3 94,097 + Atu3088 0.41 +0.1 94,296 + Atu3088 0.57 -0.9
Or see this region's nucleotide sequence