Experiment: R2A with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0133 and Atu0134 overlap by 8 nucleotides Atu0134 and Atu0135 are separated by 119 nucleotides Atu0135 and Atu0136 are separated by 95 nucleotides
Atu0133: Atu0133 - thymidine phosphorylase, at 136,629 to 137,945
Atu0133
Atu0134: Atu0134 - hypothetical protein, at 137,938 to 138,474
Atu0134
Atu0135: Atu0135 - uracil phosphoribosyltransferase, at 138,594 to 139,223
Atu0135
Atu0136: Atu0136 - adenosine deaminase, at 139,319 to 140,296
Atu0136
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 137.661 kb on + strand, within Atu0133 at 137.794 kb on + strand, within Atu0133 at 137.794 kb on + strand, within Atu0133 at 137.794 kb on + strand, within Atu0133 at 137.794 kb on + strand, within Atu0133 at 137.804 kb on + strand, within Atu0133 at 137.808 kb on - strand, within Atu0133 at 137.808 kb on - strand, within Atu0133 at 137.810 kb on - strand, within Atu0133 at 137.838 kb on + strand at 137.864 kb on + strand at 137.892 kb on - strand at 138.088 kb on + strand, within Atu0134 at 138.112 kb on + strand, within Atu0134 at 138.164 kb on + strand, within Atu0134 at 138.299 kb on + strand, within Atu0134 at 138.475 kb on + strand at 138.482 kb on + strand at 138.482 kb on + strand at 138.482 kb on + strand at 138.482 kb on + strand at 138.482 kb on + strand at 138.482 kb on + strand at 138.483 kb on - strand at 138.490 kb on - strand at 138.490 kb on - strand at 138.490 kb on - strand at 138.490 kb on - strand at 138.490 kb on - strand at 138.490 kb on - strand at 138.580 kb on - strand at 138.983 kb on - strand, within Atu0135 at 139.089 kb on + strand, within Atu0135 at 139.306 kb on + strand at 139.335 kb on + strand at 139.426 kb on - strand, within Atu0136 at 139.452 kb on - strand, within Atu0136 at 139.518 kb on - strand, within Atu0136 at 139.618 kb on + strand, within Atu0136 at 139.626 kb on - strand, within Atu0136 at 139.639 kb on + strand, within Atu0136 at 139.727 kb on + strand, within Atu0136 at 139.837 kb on + strand, within Atu0136 at 139.985 kb on - strand, within Atu0136 at 140.114 kb on - strand, within Atu0136 at 140.130 kb on + strand, within Atu0136
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 5 mM remove 137,661 + Atu0133 0.78 +0.3 137,794 + Atu0133 0.88 -0.0 137,794 + Atu0133 0.88 +0.4 137,794 + Atu0133 0.88 +0.9 137,794 + Atu0133 0.88 -0.5 137,804 + Atu0133 0.89 +0.0 137,808 - Atu0133 0.90 +0.1 137,808 - Atu0133 0.90 +1.1 137,810 - Atu0133 0.90 +0.0 137,838 + -0.5 137,864 + -0.4 137,892 - -0.4 138,088 + Atu0134 0.28 -0.1 138,112 + Atu0134 0.32 -0.0 138,164 + Atu0134 0.42 -0.2 138,299 + Atu0134 0.67 +3.6 138,475 + -0.9 138,482 + -0.8 138,482 + +0.4 138,482 + -0.1 138,482 + +0.4 138,482 + +0.2 138,482 + +0.3 138,483 - +0.7 138,490 - -0.1 138,490 - +0.6 138,490 - +0.7 138,490 - +0.1 138,490 - -0.5 138,490 - -0.1 138,580 - +0.9 138,983 - Atu0135 0.62 -1.1 139,089 + Atu0135 0.79 -2.1 139,306 + -0.1 139,335 + +0.1 139,426 - Atu0136 0.11 +0.4 139,452 - Atu0136 0.14 +0.8 139,518 - Atu0136 0.20 +1.2 139,618 + Atu0136 0.31 +0.4 139,626 - Atu0136 0.31 -0.2 139,639 + Atu0136 0.33 +0.2 139,727 + Atu0136 0.42 +0.2 139,837 + Atu0136 0.53 -1.0 139,985 - Atu0136 0.68 -0.2 140,114 - Atu0136 0.81 +0.4 140,130 + Atu0136 0.83 +0.0
Or see this region's nucleotide sequence