Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tilS and yaeO are separated by 48 nucleotides yaeO and yaeP overlap by 8 nucleotides yaeP and yaeQ are separated by 165 nucleotides yaeQ and yaeJ overlap by 4 nucleotides
b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629
tilS
b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932
yaeO
b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125
yaeP
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
Position (kb) 213
214
215 Strain fitness (log2 ratio) -2
-1
0
1 at 213.686 kb on + strand at 213.686 kb on + strand at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.716 kb on + strand, within yaeO at 213.783 kb on + strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.913 kb on + strand at 213.913 kb on + strand at 213.956 kb on - strand, within yaeP at 213.956 kb on - strand, within yaeP at 214.040 kb on - strand, within yaeP at 214.130 kb on + strand at 214.142 kb on + strand at 214.166 kb on + strand at 214.174 kb on - strand at 214.174 kb on - strand at 214.216 kb on - strand at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 213,686 + +1.4 213,686 + +0.8 213,716 + yaeO b0189 0.15 -0.7 213,716 + yaeO b0189 0.15 -0.7 213,716 + yaeO b0189 0.15 -2.4 213,716 + yaeO b0189 0.15 -0.6 213,783 + yaeO b0189 0.41 +0.5 213,880 - yaeO b0189 0.79 -0.3 213,880 - yaeO b0189 0.79 -0.4 213,913 + +1.0 213,913 + +0.8 213,956 - yaeP b4406 0.15 -0.8 213,956 - yaeP b4406 0.15 -1.5 214,040 - yaeP b4406 0.57 +0.1 214,130 + -0.3 214,142 + +0.6 214,166 + -0.4 214,174 - -1.3 214,174 - +0.2 214,216 - +0.1 214,369 - yaeQ b0190 0.14 -1.3 214,391 - yaeQ b0190 0.18 +0.6 214,404 - yaeQ b0190 0.21 +0.2 214,419 - yaeQ b0190 0.23 +0.8 214,468 + yaeQ b0190 0.32 -0.5 214,487 + yaeQ b0190 0.36 +0.2 214,487 + yaeQ b0190 0.36 +0.8 214,542 + yaeQ b0190 0.46 +0.2 214,572 + yaeQ b0190 0.51 +1.2 214,574 + yaeQ b0190 0.52 -1.0 214,600 - yaeQ b0190 0.57 -0.9 214,606 + yaeQ b0190 0.58 +0.8 214,606 + yaeQ b0190 0.58 -0.7 214,764 - yaeQ b0190 0.87 +0.3 214,773 - yaeQ b0190 0.88 +0.9 214,808 + +0.2 214,825 - -0.4 214,836 - +0.1 214,872 + -0.6 214,874 + -0.4 214,919 + yaeJ b0191 0.20 -0.8 215,045 + yaeJ b0191 0.50 +0.0 215,045 + yaeJ b0191 0.50 -1.3 215,091 + yaeJ b0191 0.61 -1.9 215,123 + yaeJ b0191 0.69 +0.5 215,123 + yaeJ b0191 0.69 +0.4
Or see this region's nucleotide sequence