Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fecA and fecR are separated by 86 nucleotides fecR and fecI overlap by 4 nucleotides fecI and insA-7 are separated by 291 nucleotides
b4291: fecA - KpLE2 phage-like element; ferric citrate outer membrane transporter (NCBI), at 4,512,376 to 4,514,700
fecA
b4292: fecR - KpLE2 phage-like element; transmembrane signal transducer for ferric citrate transport (NCBI), at 4,514,787 to 4,515,740
fecR
b4293: fecI - KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI), at 4,515,737 to 4,516,258
fecI
b4294: insA-7 - KpLE2 phage-like element; IS1 repressor protein InsA (NCBI), at 4,516,550 to 4,516,825
insA-7
Position (kb)
4514
4515
4516 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4513.792 kb on - strand, within fecA at 4513.792 kb on - strand, within fecA at 4513.808 kb on + strand, within fecA at 4513.821 kb on - strand, within fecA at 4513.821 kb on - strand, within fecA at 4513.847 kb on - strand, within fecA at 4513.854 kb on + strand, within fecA at 4513.901 kb on - strand, within fecA at 4513.934 kb on - strand, within fecA at 4513.961 kb on - strand, within fecA at 4513.991 kb on + strand, within fecA at 4514.038 kb on - strand, within fecA at 4514.038 kb on - strand, within fecA at 4514.078 kb on + strand, within fecA at 4514.097 kb on - strand, within fecA at 4514.110 kb on + strand, within fecA at 4514.281 kb on + strand, within fecA at 4514.281 kb on + strand, within fecA at 4514.304 kb on + strand, within fecA at 4514.304 kb on + strand, within fecA at 4514.427 kb on - strand, within fecA at 4514.427 kb on - strand, within fecA at 4514.468 kb on + strand at 4514.523 kb on - strand at 4514.523 kb on - strand at 4514.544 kb on + strand at 4514.544 kb on + strand at 4514.547 kb on - strand at 4514.549 kb on + strand at 4514.549 kb on + strand at 4514.552 kb on - strand at 4514.556 kb on - strand at 4514.556 kb on - strand at 4514.577 kb on + strand at 4514.581 kb on - strand at 4514.588 kb on + strand at 4514.588 kb on + strand at 4514.588 kb on + strand at 4514.596 kb on - strand at 4514.596 kb on - strand at 4514.598 kb on + strand at 4514.740 kb on - strand at 4514.962 kb on - strand, within fecR at 4515.006 kb on - strand, within fecR at 4515.017 kb on + strand, within fecR at 4515.044 kb on + strand, within fecR at 4515.059 kb on - strand, within fecR at 4515.152 kb on - strand, within fecR at 4515.299 kb on - strand, within fecR at 4515.303 kb on + strand, within fecR at 4515.311 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.522 kb on + strand, within fecR at 4515.546 kb on + strand, within fecR at 4515.584 kb on - strand, within fecR at 4515.609 kb on - strand, within fecR at 4515.647 kb on + strand at 4515.669 kb on + strand at 4515.669 kb on + strand at 4515.702 kb on - strand at 4515.749 kb on + strand at 4515.756 kb on - strand at 4515.760 kb on + strand at 4515.770 kb on + strand at 4515.852 kb on - strand, within fecI at 4515.928 kb on - strand, within fecI at 4515.928 kb on - strand, within fecI at 4515.997 kb on + strand, within fecI at 4516.053 kb on + strand, within fecI at 4516.053 kb on + strand, within fecI at 4516.059 kb on + strand, within fecI at 4516.059 kb on + strand, within fecI at 4516.067 kb on - strand, within fecI at 4516.067 kb on - strand, within fecI at 4516.111 kb on - strand, within fecI at 4516.116 kb on - strand, within fecI at 4516.135 kb on - strand, within fecI at 4516.377 kb on + strand at 4516.417 kb on + strand at 4516.430 kb on + strand at 4516.456 kb on + strand at 4516.515 kb on + strand at 4516.515 kb on + strand at 4516.686 kb on - strand, within insA-7 at 4516.686 kb on - strand, within insA-7
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 4,513,792 - fecA b4291 0.61 +0.9 4,513,792 - fecA b4291 0.61 +1.5 4,513,808 + fecA b4291 0.62 +2.1 4,513,821 - fecA b4291 0.62 +2.2 4,513,821 - fecA b4291 0.62 +2.2 4,513,847 - fecA b4291 0.63 +1.1 4,513,854 + fecA b4291 0.64 +1.4 4,513,901 - fecA b4291 0.66 +2.5 4,513,934 - fecA b4291 0.67 +1.2 4,513,961 - fecA b4291 0.68 +0.8 4,513,991 + fecA b4291 0.69 +1.1 4,514,038 - fecA b4291 0.71 +2.0 4,514,038 - fecA b4291 0.71 +1.7 4,514,078 + fecA b4291 0.73 +1.8 4,514,097 - fecA b4291 0.74 +1.4 4,514,110 + fecA b4291 0.75 +2.3 4,514,281 + fecA b4291 0.82 +2.3 4,514,281 + fecA b4291 0.82 +1.6 4,514,304 + fecA b4291 0.83 +2.1 4,514,304 + fecA b4291 0.83 +3.4 4,514,427 - fecA b4291 0.88 +1.9 4,514,427 - fecA b4291 0.88 +2.1 4,514,468 + +3.3 4,514,523 - +1.8 4,514,523 - +1.4 4,514,544 + +3.1 4,514,544 + +2.1 4,514,547 - +1.7 4,514,549 + +2.9 4,514,549 + +1.7 4,514,552 - +1.9 4,514,556 - +1.7 4,514,556 - +3.3 4,514,577 + +1.9 4,514,581 - +2.6 4,514,588 + +1.6 4,514,588 + +1.3 4,514,588 + +2.1 4,514,596 - +2.0 4,514,596 - +2.2 4,514,598 + +2.2 4,514,740 - -2.1 4,514,962 - fecR b4292 0.18 +1.2 4,515,006 - fecR b4292 0.23 +2.1 4,515,017 + fecR b4292 0.24 +1.6 4,515,044 + fecR b4292 0.27 +0.9 4,515,059 - fecR b4292 0.29 +2.7 4,515,152 - fecR b4292 0.38 +2.5 4,515,299 - fecR b4292 0.54 +1.9 4,515,303 + fecR b4292 0.54 +2.2 4,515,311 - fecR b4292 0.55 +2.9 4,515,504 - fecR b4292 0.75 +2.2 4,515,504 - fecR b4292 0.75 +1.7 4,515,522 + fecR b4292 0.77 +2.0 4,515,546 + fecR b4292 0.80 +2.0 4,515,584 - fecR b4292 0.84 +2.3 4,515,609 - fecR b4292 0.86 +2.8 4,515,647 + +2.4 4,515,669 + +3.5 4,515,669 + +2.3 4,515,702 - +2.5 4,515,749 + +1.9 4,515,756 - +2.0 4,515,760 + +2.5 4,515,770 + +2.1 4,515,852 - fecI b4293 0.22 +1.8 4,515,928 - fecI b4293 0.37 +2.3 4,515,928 - fecI b4293 0.37 +3.5 4,515,997 + fecI b4293 0.50 +2.6 4,516,053 + fecI b4293 0.61 +1.1 4,516,053 + fecI b4293 0.61 +0.9 4,516,059 + fecI b4293 0.62 +2.0 4,516,059 + fecI b4293 0.62 +4.7 4,516,067 - fecI b4293 0.63 +1.2 4,516,067 - fecI b4293 0.63 +1.3 4,516,111 - fecI b4293 0.72 +1.9 4,516,116 - fecI b4293 0.73 +1.4 4,516,135 - fecI b4293 0.76 +2.4 4,516,377 + -0.6 4,516,417 + +0.5 4,516,430 + -2.2 4,516,456 + -1.7 4,516,515 + -0.7 4,516,515 + -0.1 4,516,686 - insA-7 b4294 0.49 -0.2 4,516,686 - insA-7 b4294 0.49 -0.6
Or see this region's nucleotide sequence