Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqiB and nudF are separated by 0 nucleotides nudF and tolC are separated by 204 nucleotides tolC and ygiB are separated by 147 nucleotides ygiB and ygiC are separated by 5 nucleotides
b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302
yqiB
b3034: nudF - ADP-ribose pyrophosphatase (NCBI), at 3,175,303 to 3,175,932
nudF
b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618
tolC
b3037: ygiB - conserved outer membrane protein (RefSeq), at 3,177,766 to 3,178,437
ygiB
b3038: ygiC - predicted enzyme (NCBI), at 3,178,443 to 3,179,603
ygiC
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3175.171 kb on - strand, within yqiB at 3175.226 kb on + strand, within yqiB at 3175.399 kb on - strand, within nudF at 3175.469 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.528 kb on - strand, within nudF at 3175.577 kb on + strand, within nudF at 3175.585 kb on + strand, within nudF at 3175.590 kb on - strand, within nudF at 3175.662 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.752 kb on - strand, within nudF at 3175.798 kb on + strand, within nudF at 3175.818 kb on - strand, within nudF at 3175.823 kb on - strand, within nudF at 3175.871 kb on - strand at 3176.030 kb on - strand at 3176.031 kb on + strand at 3176.119 kb on - strand at 3176.119 kb on - strand at 3176.173 kb on - strand at 3176.173 kb on - strand at 3176.174 kb on + strand at 3176.182 kb on - strand at 3176.208 kb on - strand at 3176.271 kb on + strand at 3176.271 kb on + strand at 3176.304 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.349 kb on - strand, within tolC at 3176.378 kb on + strand, within tolC at 3176.493 kb on + strand, within tolC at 3176.526 kb on - strand, within tolC at 3176.571 kb on + strand, within tolC at 3176.573 kb on + strand, within tolC at 3176.581 kb on - strand, within tolC at 3176.635 kb on + strand, within tolC at 3176.638 kb on - strand, within tolC at 3176.654 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.682 kb on + strand, within tolC at 3176.692 kb on - strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.932 kb on + strand, within tolC at 3176.934 kb on + strand, within tolC at 3176.942 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.988 kb on - strand, within tolC at 3177.093 kb on + strand, within tolC at 3177.105 kb on + strand, within tolC at 3177.113 kb on - strand, within tolC at 3177.120 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.437 kb on + strand, within tolC at 3177.488 kb on + strand at 3177.516 kb on - strand at 3177.517 kb on + strand at 3177.517 kb on + strand at 3177.717 kb on + strand at 3177.737 kb on + strand at 3177.747 kb on - strand at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on - strand, within ygiB at 3178.163 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.294 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.390 kb on + strand at 3178.390 kb on + strand at 3178.398 kb on - strand at 3178.423 kb on - strand at 3178.454 kb on + strand at 3178.540 kb on - strand at 3178.542 kb on + strand at 3178.545 kb on - strand at 3178.546 kb on - strand at 3178.546 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 3,175,171 - yqiB b3033 0.69 +1.7 3,175,226 + yqiB b3033 0.82 -1.3 3,175,399 - nudF b3034 0.15 +2.2 3,175,469 - nudF b3034 0.26 +1.6 3,175,494 - nudF b3034 0.30 +0.9 3,175,494 - nudF b3034 0.30 +2.0 3,175,528 - nudF b3034 0.36 +3.1 3,175,577 + nudF b3034 0.43 -0.2 3,175,585 + nudF b3034 0.45 -0.2 3,175,590 - nudF b3034 0.46 +2.1 3,175,662 + nudF b3034 0.57 +0.4 3,175,679 + nudF b3034 0.60 +0.5 3,175,679 + nudF b3034 0.60 +0.6 3,175,752 - nudF b3034 0.71 +1.4 3,175,798 + nudF b3034 0.79 +0.7 3,175,818 - nudF b3034 0.82 +0.8 3,175,823 - nudF b3034 0.83 +1.4 3,175,871 - +1.5 3,176,030 - +0.1 3,176,031 + -1.0 3,176,119 - -2.4 3,176,119 - +2.0 3,176,173 - -3.3 3,176,173 - -2.5 3,176,174 + -2.6 3,176,182 - +0.0 3,176,208 - -1.7 3,176,271 + -3.4 3,176,271 + -2.8 3,176,304 + tolC b3035 0.11 -1.9 3,176,309 + tolC b3035 0.12 -1.9 3,176,309 + tolC b3035 0.12 -1.8 3,176,349 - tolC b3035 0.14 -1.2 3,176,378 + tolC b3035 0.16 -2.9 3,176,493 + tolC b3035 0.24 -0.2 3,176,526 - tolC b3035 0.26 -2.9 3,176,571 + tolC b3035 0.29 -1.8 3,176,573 + tolC b3035 0.29 -1.9 3,176,581 - tolC b3035 0.30 -0.1 3,176,635 + tolC b3035 0.34 -1.5 3,176,638 - tolC b3035 0.34 -0.8 3,176,654 - tolC b3035 0.35 -2.4 3,176,680 - tolC b3035 0.37 -1.3 3,176,680 - tolC b3035 0.37 -1.3 3,176,682 + tolC b3035 0.37 -1.6 3,176,692 - tolC b3035 0.37 -3.8 3,176,716 + tolC b3035 0.39 -2.2 3,176,716 + tolC b3035 0.39 -1.8 3,176,844 - tolC b3035 0.48 -0.3 3,176,844 - tolC b3035 0.48 -1.0 3,176,869 - tolC b3035 0.49 -2.0 3,176,869 - tolC b3035 0.49 -1.9 3,176,887 + tolC b3035 0.51 -2.2 3,176,887 + tolC b3035 0.51 -2.1 3,176,887 + tolC b3035 0.51 -3.7 3,176,932 + tolC b3035 0.54 -2.8 3,176,934 + tolC b3035 0.54 -1.5 3,176,942 - tolC b3035 0.54 -3.9 3,176,979 - tolC b3035 0.57 -1.2 3,176,979 - tolC b3035 0.57 +0.4 3,176,981 + tolC b3035 0.57 -3.6 3,176,981 + tolC b3035 0.57 -0.3 3,176,988 - tolC b3035 0.57 -2.2 3,177,093 + tolC b3035 0.65 -3.1 3,177,105 + tolC b3035 0.65 -2.6 3,177,113 - tolC b3035 0.66 -0.9 3,177,120 + tolC b3035 0.66 -3.2 3,177,339 + tolC b3035 0.81 -0.7 3,177,339 + tolC b3035 0.81 -0.6 3,177,437 + tolC b3035 0.88 -3.2 3,177,488 + -1.2 3,177,516 - -1.8 3,177,517 + -1.4 3,177,517 + -2.6 3,177,717 + +0.3 3,177,737 + +0.1 3,177,747 - -1.1 3,177,964 + ygiB b3037 0.29 -1.3 3,177,964 + ygiB b3037 0.29 -0.3 3,177,964 + ygiB b3037 0.29 +0.8 3,177,964 - ygiB b3037 0.29 +0.1 3,178,163 + ygiB b3037 0.59 +0.4 3,178,238 + ygiB b3037 0.70 +0.8 3,178,238 + ygiB b3037 0.70 -0.2 3,178,276 + ygiB b3037 0.76 +1.3 3,178,276 + ygiB b3037 0.76 +0.4 3,178,294 - ygiB b3037 0.79 -0.3 3,178,298 - ygiB b3037 0.79 +0.1 3,178,298 - ygiB b3037 0.79 +0.0 3,178,390 + -0.3 3,178,390 + -2.2 3,178,398 - -0.3 3,178,423 - -0.9 3,178,454 + -0.4 3,178,540 - +0.3 3,178,542 + +0.2 3,178,545 - -0.7 3,178,546 - +0.3 3,178,546 - +0.3
Or see this region's nucleotide sequence