Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menC and menB overlap by 1 nucleotides menB and yfbB are separated by 14 nucleotides yfbB and menD overlap by 4 nucleotides
b2261: menC - O-succinylbenzoate synthase (NCBI), at 2,373,022 to 2,373,984
menC
b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841
menB
b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614
yfbB
b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281
menD
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-2
-1
0
1 at 2373.925 kb on + strand at 2373.930 kb on + strand at 2373.930 kb on + strand at 2374.136 kb on + strand, within menB at 2374.254 kb on - strand, within menB at 2374.373 kb on + strand, within menB at 2374.491 kb on + strand, within menB at 2374.673 kb on - strand, within menB at 2374.831 kb on + strand at 2374.831 kb on + strand at 2374.885 kb on + strand at 2374.893 kb on - strand at 2375.223 kb on - strand, within yfbB at 2375.307 kb on - strand, within yfbB at 2375.363 kb on - strand, within yfbB at 2375.570 kb on + strand at 2375.570 kb on + strand at 2375.616 kb on - strand at 2375.616 kb on - strand at 2375.795 kb on - strand, within menD at 2376.112 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.143 kb on - strand, within menD at 2376.359 kb on + strand, within menD at 2376.359 kb on + strand, within menD at 2376.515 kb on + strand, within menD at 2376.554 kb on - strand, within menD at 2376.585 kb on + strand, within menD at 2376.585 kb on + strand, within menD at 2376.605 kb on + strand, within menD
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 2,373,925 + -0.0 2,373,930 + +0.3 2,373,930 + -1.0 2,374,136 + menB b2262 0.18 -1.2 2,374,254 - menB b2262 0.31 +0.1 2,374,373 + menB b2262 0.45 +0.3 2,374,491 + menB b2262 0.59 +0.4 2,374,673 - menB b2262 0.80 +0.6 2,374,831 + -1.6 2,374,831 + -0.2 2,374,885 + -0.4 2,374,893 - +1.0 2,375,223 - yfbB b2263 0.48 +0.3 2,375,307 - yfbB b2263 0.59 -2.1 2,375,363 - yfbB b2263 0.67 -1.9 2,375,570 + +0.9 2,375,570 + -1.1 2,375,616 - -1.4 2,375,616 - +1.1 2,375,795 - menD b2264 0.11 -1.0 2,376,112 - menD b2264 0.30 -1.5 2,376,143 - menD b2264 0.32 -0.6 2,376,143 - menD b2264 0.32 -0.5 2,376,359 + menD b2264 0.45 -0.1 2,376,359 + menD b2264 0.45 +1.5 2,376,515 + menD b2264 0.54 +0.2 2,376,554 - menD b2264 0.56 -0.5 2,376,585 + menD b2264 0.58 -0.2 2,376,585 + menD b2264 0.58 +0.4 2,376,605 + menD b2264 0.59 -0.7
Or see this region's nucleotide sequence