Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaC and wcaB overlap by 4 nucleotides wcaB and wcaA are separated by 2 nucleotides wcaA and b2060 are separated by 92 nucleotides
b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094
wcaC
b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579
wcaB
b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421
wcaA
b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676
b2060
Position (kb)
2130
2131 Strain fitness (log2 ratio)
-1
0
1 at 2129.249 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.468 kb on + strand, within wcaC at 2129.473 kb on + strand, within wcaC at 2129.586 kb on + strand, within wcaC at 2129.690 kb on + strand, within wcaC at 2129.798 kb on + strand, within wcaC at 2129.803 kb on + strand, within wcaC at 2130.018 kb on - strand at 2130.025 kb on + strand at 2130.025 kb on + strand at 2130.046 kb on - strand at 2130.159 kb on + strand, within wcaB at 2130.625 kb on + strand at 2130.691 kb on - strand, within wcaA at 2130.691 kb on - strand, within wcaA at 2130.692 kb on - strand, within wcaA at 2130.813 kb on + strand, within wcaA at 2130.841 kb on + strand, within wcaA at 2130.889 kb on - strand, within wcaA at 2131.196 kb on - strand, within wcaA at 2131.212 kb on + strand, within wcaA at 2131.306 kb on - strand, within wcaA at 2131.332 kb on - strand, within wcaA at 2131.395 kb on + strand at 2131.395 kb on + strand at 2131.421 kb on - strand at 2131.519 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 2,129,249 - wcaC b2057 0.31 +0.6 2,129,249 - wcaC b2057 0.31 +0.9 2,129,459 + wcaC b2057 0.48 +1.2 2,129,459 + wcaC b2057 0.48 +0.6 2,129,468 + wcaC b2057 0.49 -0.6 2,129,473 + wcaC b2057 0.49 -0.1 2,129,586 + wcaC b2057 0.58 +0.2 2,129,690 + wcaC b2057 0.67 -0.6 2,129,798 + wcaC b2057 0.76 -0.2 2,129,803 + wcaC b2057 0.76 +0.3 2,130,018 - +0.0 2,130,025 + +0.0 2,130,025 + -0.8 2,130,046 - +0.6 2,130,159 + wcaB b2058 0.14 -0.5 2,130,625 + +0.5 2,130,691 - wcaA b2059 0.13 +1.4 2,130,691 - wcaA b2059 0.13 -0.6 2,130,692 - wcaA b2059 0.13 +0.1 2,130,813 + wcaA b2059 0.28 +1.2 2,130,841 + wcaA b2059 0.31 -0.4 2,130,889 - wcaA b2059 0.37 +0.1 2,131,196 - wcaA b2059 0.73 +0.4 2,131,212 + wcaA b2059 0.75 +0.6 2,131,306 - wcaA b2059 0.86 -0.7 2,131,332 - wcaA b2059 0.89 -0.8 2,131,395 + -1.0 2,131,395 + +0.1 2,131,421 - -0.3 2,131,519 - -0.2
Or see this region's nucleotide sequence