Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yniC and ydjM are separated by 162 nucleotides ydjM and ydjN are separated by 132 nucleotides ydjN and ydjO are separated by 3 nucleotides
b1727: yniC - predicted hydrolase (NCBI), at 1,807,404 to 1,808,072
yniC
b1728: ydjM - predicted inner membrane protein regulated by LexA (RefSeq), at 1,808,235 to 1,808,825
ydjM
b1729: ydjN - predicted transporter (NCBI), at 1,808,958 to 1,810,349
ydjN
b1730: ydjO - predicted protein (RefSeq), at 1,810,353 to 1,811,156
ydjO
Position (kb)
1808
1809
1810
1811 Strain fitness (log2 ratio)
-1
0
1 at 1808.090 kb on + strand at 1808.185 kb on - strand at 1808.203 kb on - strand at 1808.359 kb on + strand, within ydjM at 1808.524 kb on - strand, within ydjM at 1808.524 kb on - strand, within ydjM at 1808.679 kb on + strand, within ydjM at 1808.870 kb on + strand at 1808.924 kb on - strand at 1809.193 kb on + strand, within ydjN at 1809.214 kb on + strand, within ydjN at 1809.242 kb on + strand, within ydjN at 1809.242 kb on + strand, within ydjN at 1809.720 kb on + strand, within ydjN at 1809.720 kb on + strand, within ydjN at 1810.253 kb on + strand at 1810.253 kb on + strand at 1810.284 kb on + strand at 1810.284 kb on + strand at 1810.343 kb on + strand at 1810.445 kb on - strand, within ydjO at 1810.493 kb on + strand, within ydjO at 1810.515 kb on + strand, within ydjO at 1811.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 1,808,090 + -0.5 1,808,185 - -0.2 1,808,203 - -0.7 1,808,359 + ydjM b1728 0.21 -0.8 1,808,524 - ydjM b1728 0.49 -0.8 1,808,524 - ydjM b1728 0.49 +0.9 1,808,679 + ydjM b1728 0.75 -0.0 1,808,870 + -0.1 1,808,924 - -0.3 1,809,193 + ydjN b1729 0.17 -0.7 1,809,214 + ydjN b1729 0.18 -1.7 1,809,242 + ydjN b1729 0.20 -1.1 1,809,242 + ydjN b1729 0.20 -0.3 1,809,720 + ydjN b1729 0.55 -1.2 1,809,720 + ydjN b1729 0.55 +0.1 1,810,253 + +1.2 1,810,253 + -0.1 1,810,284 + +0.3 1,810,284 + -0.5 1,810,343 + +0.0 1,810,445 - ydjO b1730 0.11 +0.2 1,810,493 + ydjO b1730 0.17 -1.2 1,810,515 + ydjO b1730 0.20 -1.0 1,811,304 - -0.2
Or see this region's nucleotide sequence