Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgI and minE are separated by 371 nucleotides minE and minD are separated by 3 nucleotides minD and minC are separated by 23 nucleotides
b4521: ycgI - hypothetical protein (NCBI), at 1,222,787 to 1,223,130
ycgI
b1174: minE - cell division topological specificity factor MinE (NCBI), at 1,223,502 to 1,223,768
minE
b1175: minD - membrane ATPase of the MinC-MinD-MinE system (NCBI), at 1,223,772 to 1,224,584
minD
b1176: minC - septum formation inhibitor (NCBI), at 1,224,608 to 1,225,303
minC
Position (kb)
1223
1224
1225 Strain fitness (log2 ratio)
-1
0
1 at 1222.783 kb on + strand at 1222.846 kb on + strand, within ycgI at 1222.857 kb on - strand, within ycgI at 1222.981 kb on + strand, within ycgI at 1223.000 kb on + strand, within ycgI at 1223.000 kb on + strand, within ycgI at 1223.354 kb on + strand at 1223.354 kb on + strand at 1223.354 kb on + strand at 1223.362 kb on - strand at 1223.362 kb on - strand at 1223.403 kb on - strand at 1223.405 kb on + strand at 1223.432 kb on + strand at 1223.440 kb on - strand at 1223.507 kb on - strand at 1223.507 kb on - strand at 1223.513 kb on - strand at 1223.527 kb on - strand at 1223.902 kb on - strand, within minD at 1223.936 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1224.022 kb on - strand, within minD at 1224.061 kb on + strand, within minD at 1224.461 kb on - strand, within minD at 1224.461 kb on - strand, within minD at 1224.509 kb on - strand at 1224.534 kb on + strand at 1224.577 kb on - strand at 1224.684 kb on + strand, within minC at 1224.784 kb on + strand, within minC at 1224.854 kb on - strand, within minC at 1225.133 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.310 kb on - strand at 1225.310 kb on - strand at 1225.329 kb on + strand at 1225.354 kb on - strand at 1225.380 kb on - strand at 1225.382 kb on + strand at 1225.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 1,222,783 + +0.0 1,222,846 + ycgI b4521 0.17 -0.5 1,222,857 - ycgI b4521 0.20 -0.1 1,222,981 + ycgI b4521 0.56 +0.5 1,223,000 + ycgI b4521 0.62 -0.9 1,223,000 + ycgI b4521 0.62 -0.2 1,223,354 + +0.9 1,223,354 + -0.0 1,223,354 + +0.4 1,223,362 - -0.3 1,223,362 - +0.3 1,223,403 - +0.4 1,223,405 + +0.7 1,223,432 + -0.3 1,223,440 - -0.0 1,223,507 - +0.2 1,223,507 - +0.8 1,223,513 - -1.2 1,223,527 - -0.4 1,223,902 - minD b1175 0.16 -0.8 1,223,936 - minD b1175 0.20 -1.0 1,223,941 - minD b1175 0.21 -1.5 1,223,941 - minD b1175 0.21 -0.3 1,224,022 - minD b1175 0.31 -0.6 1,224,061 + minD b1175 0.36 -0.8 1,224,461 - minD b1175 0.85 -0.6 1,224,461 - minD b1175 0.85 -0.0 1,224,509 - -0.1 1,224,534 + -0.9 1,224,577 - +0.4 1,224,684 + minC b1176 0.11 -0.2 1,224,784 + minC b1176 0.25 -0.7 1,224,854 - minC b1176 0.35 -0.3 1,225,133 - minC b1176 0.75 -0.6 1,225,151 - minC b1176 0.78 -1.5 1,225,151 - minC b1176 0.78 +0.6 1,225,310 - -0.3 1,225,310 - +0.1 1,225,329 + -1.0 1,225,354 - +0.2 1,225,380 - -1.0 1,225,382 + -0.4 1,225,405 - +0.6
Or see this region's nucleotide sequence