Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides moaE and ybhL are separated by 136 nucleotides
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
b0786: ybhL - predicted inner membrane protein (NCBI), at 819,107 to 819,811
ybhL
Position (kb)
818
819 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE at 818.892 kb on - strand, within moaE at 818.926 kb on + strand at 818.940 kb on - strand at 819.022 kb on + strand at 819.025 kb on - strand at 819.154 kb on + strand at 819.210 kb on + strand, within ybhL at 819.259 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.354 kb on + strand, within ybhL at 819.408 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.493 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 817,280 - -0.5 817,280 - -0.6 817,355 - moaB b0782 0.15 +0.3 817,485 + moaB b0782 0.40 -1.1 817,485 + moaB b0782 0.40 +0.7 817,818 - -0.2 817,818 - +0.1 817,852 - moaC b0783 0.12 -0.3 817,852 - moaC b0783 0.12 +0.2 817,872 - moaC b0783 0.16 +0.0 817,912 + moaC b0783 0.24 +0.3 817,912 + moaC b0783 0.24 +1.5 818,006 - moaC b0783 0.44 -0.1 818,012 - moaC b0783 0.45 +0.5 818,167 - moaC b0783 0.77 -0.6 818,211 + moaC b0783 0.86 -0.1 818,219 - moaC b0783 0.88 -0.6 818,219 - moaC b0783 0.88 -0.4 818,252 + +0.2 818,252 + +0.3 818,369 + moaD b0784 0.40 -1.2 818,455 + moaD b0784 0.75 +0.6 818,455 + moaD b0784 0.75 -2.8 818,566 - moaE b0785 0.11 +0.8 818,566 - moaE b0785 0.11 +0.5 818,566 - moaE b0785 0.11 +0.9 818,677 - moaE b0785 0.35 -1.1 818,687 - moaE b0785 0.37 -0.3 818,764 - moaE b0785 0.54 -0.1 818,892 - moaE b0785 0.83 +0.1 818,926 + -0.5 818,940 - -0.1 819,022 + +0.1 819,025 - +0.4 819,154 + +1.0 819,210 + ybhL b0786 0.15 -1.3 819,259 + ybhL b0786 0.22 +0.8 819,314 + ybhL b0786 0.29 +0.2 819,314 + ybhL b0786 0.29 -0.3 819,354 + ybhL b0786 0.35 +1.3 819,408 + ybhL b0786 0.43 -0.0 819,490 + ybhL b0786 0.54 +0.5 819,490 + ybhL b0786 0.54 +0.2 819,493 + ybhL b0786 0.55 -1.0 819,500 + ybhL b0786 0.56 +0.6 819,500 + ybhL b0786 0.56 -0.2
Or see this region's nucleotide sequence