Experiment: limonene 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and cyoD are separated by 11 nucleotides cyoD and cyoC overlap by 1 nucleotides cyoC and cyoB overlap by 11 nucleotides cyoB and cyoA are separated by 21 nucleotides
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
Position (kb) 447
448
449
450 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 446.880 kb on + strand at 446.880 kb on + strand at 446.932 kb on + strand at 446.944 kb on + strand at 446.981 kb on - strand, within cyoD at 447.018 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.105 kb on - strand, within cyoD at 447.161 kb on - strand, within cyoD at 447.167 kb on + strand, within cyoD at 447.175 kb on - strand, within cyoD at 447.201 kb on + strand, within cyoD at 447.223 kb on - strand, within cyoD at 447.285 kb on - strand at 447.303 kb on - strand at 447.347 kb on + strand, within cyoC at 447.351 kb on + strand, within cyoC at 447.373 kb on - strand, within cyoC at 447.407 kb on + strand, within cyoC at 447.418 kb on - strand, within cyoC at 447.445 kb on - strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.521 kb on + strand, within cyoC at 447.570 kb on + strand, within cyoC at 447.580 kb on + strand, within cyoC at 447.679 kb on + strand, within cyoC at 447.956 kb on + strand at 447.964 kb on - strand at 447.964 kb on - strand at 447.989 kb on + strand at 448.002 kb on - strand at 448.005 kb on + strand at 448.092 kb on - strand, within cyoB at 448.092 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.238 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.434 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.455 kb on - strand, within cyoB at 448.457 kb on + strand, within cyoB at 448.466 kb on + strand, within cyoB at 448.549 kb on + strand, within cyoB at 448.557 kb on - strand, within cyoB at 448.641 kb on - strand, within cyoB at 448.678 kb on + strand, within cyoB at 448.722 kb on + strand, within cyoB at 448.722 kb on - strand, within cyoB at 448.730 kb on - strand, within cyoB at 448.750 kb on - strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.846 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on - strand, within cyoB at 448.880 kb on + strand, within cyoB at 449.038 kb on + strand, within cyoB at 449.041 kb on - strand, within cyoB at 449.053 kb on - strand, within cyoB at 449.056 kb on - strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.135 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.202 kb on + strand at 449.210 kb on - strand, within cyoB at 449.210 kb on - strand, within cyoB at 449.278 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.323 kb on + strand, within cyoB at 449.330 kb on + strand, within cyoB at 449.438 kb on - strand, within cyoB at 449.580 kb on - strand, within cyoB at 449.631 kb on + strand, within cyoB at 449.681 kb on + strand at 449.686 kb on - strand at 449.686 kb on - strand at 449.690 kb on + strand at 449.711 kb on + strand at 449.729 kb on + strand at 449.756 kb on + strand at 449.773 kb on - strand at 449.773 kb on - strand at 449.776 kb on + strand at 449.783 kb on - strand at 449.785 kb on + strand at 449.785 kb on + strand at 449.817 kb on + strand at 449.830 kb on + strand at 449.848 kb on - strand at 449.882 kb on + strand at 449.882 kb on + strand at 449.892 kb on - strand at 449.892 kb on - strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.916 kb on - strand at 449.975 kb on + strand at 449.976 kb on - strand at 449.977 kb on - strand at 449.977 kb on - strand at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.987 kb on + strand, within cyoA at 450.027 kb on - strand, within cyoA at 450.047 kb on + strand, within cyoA at 450.112 kb on - strand, within cyoA at 450.178 kb on - strand, within cyoA at 450.201 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.353 kb on + strand, within cyoA at 450.359 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.397 kb on + strand, within cyoA at 450.423 kb on - strand, within cyoA at 450.432 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.463 kb on + strand, within cyoA at 450.497 kb on - strand, within cyoA at 450.514 kb on + strand, within cyoA at 450.522 kb on + strand, within cyoA at 450.546 kb on - strand, within cyoA at 450.553 kb on + strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.575 kb on + strand, within cyoA at 450.581 kb on - strand, within cyoA at 450.597 kb on + strand, within cyoA at 450.601 kb on + strand, within cyoA at 450.601 kb on - strand, within cyoA at 450.673 kb on - strand, within cyoA at 450.687 kb on + strand, within cyoA at 450.702 kb on + strand, within cyoA at 450.707 kb on + strand, within cyoA at 450.710 kb on - strand, within cyoA at 450.715 kb on - strand, within cyoA at 450.718 kb on + strand, within cyoA at 450.725 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.753 kb on - strand at 450.763 kb on - strand at 450.769 kb on + strand at 450.769 kb on + strand at 450.774 kb on + strand at 450.777 kb on - strand at 450.779 kb on - strand at 450.779 kb on - strand at 450.782 kb on - strand at 450.782 kb on - strand at 450.792 kb on + strand at 450.863 kb on - strand at 450.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction limonene 5 vol% remove 446,880 + -1.3 446,880 + -0.5 446,932 + -2.1 446,944 + +1.2 446,981 - cyoD b0429 0.12 -0.5 447,018 - cyoD b0429 0.23 -0.4 447,093 - cyoD b0429 0.46 -0.3 447,093 - cyoD b0429 0.46 -1.0 447,105 - cyoD b0429 0.50 -1.8 447,161 - cyoD b0429 0.67 +0.2 447,167 + cyoD b0429 0.68 -0.7 447,175 - cyoD b0429 0.71 -0.5 447,201 + cyoD b0429 0.79 -0.3 447,223 - cyoD b0429 0.85 -0.5 447,285 - -0.7 447,303 - -3.0 447,347 + cyoC b0430 0.13 +0.2 447,351 + cyoC b0430 0.13 -0.1 447,373 - cyoC b0430 0.17 -0.2 447,407 + cyoC b0430 0.22 -1.0 447,418 - cyoC b0430 0.24 -1.2 447,445 - cyoC b0430 0.28 -0.5 447,492 + cyoC b0430 0.36 -0.5 447,492 + cyoC b0430 0.36 +0.7 447,521 + cyoC b0430 0.41 -1.7 447,570 + cyoC b0430 0.49 -1.0 447,580 + cyoC b0430 0.50 -1.5 447,679 + cyoC b0430 0.67 -0.7 447,956 + -0.6 447,964 - +0.4 447,964 - +0.4 447,989 + -1.0 448,002 - -1.2 448,005 + +0.1 448,092 - cyoB b0431 0.11 -1.8 448,092 - cyoB b0431 0.11 +0.4 448,134 - cyoB b0431 0.13 -0.0 448,134 - cyoB b0431 0.13 +0.2 448,238 - cyoB b0431 0.18 -3.1 448,364 - cyoB b0431 0.25 -3.0 448,364 - cyoB b0431 0.25 +0.3 448,388 + cyoB b0431 0.26 +1.0 448,388 + cyoB b0431 0.26 -1.0 448,388 + cyoB b0431 0.26 -1.4 448,391 - cyoB b0431 0.26 -0.4 448,391 - cyoB b0431 0.26 -1.5 448,396 - cyoB b0431 0.26 -1.1 448,396 - cyoB b0431 0.26 +1.4 448,396 - cyoB b0431 0.26 +1.0 448,434 - cyoB b0431 0.28 -0.3 448,443 - cyoB b0431 0.29 +0.2 448,443 - cyoB b0431 0.29 +1.0 448,455 - cyoB b0431 0.29 -0.5 448,457 + cyoB b0431 0.29 -0.8 448,466 + cyoB b0431 0.30 -0.9 448,549 + cyoB b0431 0.34 -1.4 448,557 - cyoB b0431 0.34 -1.1 448,641 - cyoB b0431 0.39 -1.8 448,678 + cyoB b0431 0.40 +0.5 448,722 + cyoB b0431 0.43 -0.9 448,722 - cyoB b0431 0.43 -0.8 448,730 - cyoB b0431 0.43 -0.3 448,750 - cyoB b0431 0.44 -1.8 448,844 + cyoB b0431 0.49 +0.2 448,844 + cyoB b0431 0.49 +2.0 448,846 + cyoB b0431 0.49 -0.6 448,854 + cyoB b0431 0.49 +0.3 448,854 + cyoB b0431 0.49 +0.3 448,854 - cyoB b0431 0.49 -2.4 448,880 + cyoB b0431 0.51 -0.0 449,038 + cyoB b0431 0.58 -0.7 449,041 - cyoB b0431 0.59 -0.5 449,053 - cyoB b0431 0.59 -1.4 449,056 - cyoB b0431 0.59 -0.6 449,098 + cyoB b0431 0.61 -0.7 449,098 + cyoB b0431 0.61 +0.8 449,135 + cyoB b0431 0.63 +1.0 449,169 + cyoB b0431 0.65 -1.0 449,169 + cyoB b0431 0.65 -0.5 449,202 + +0.4 449,210 - cyoB b0431 0.67 +1.4 449,210 - cyoB b0431 0.67 -0.7 449,278 + cyoB b0431 0.70 +0.2 449,299 + cyoB b0431 0.72 -0.0 449,299 + cyoB b0431 0.72 +0.7 449,323 + cyoB b0431 0.73 -0.5 449,330 + cyoB b0431 0.73 -0.4 449,438 - cyoB b0431 0.79 -1.2 449,580 - cyoB b0431 0.86 +0.1 449,631 + cyoB b0431 0.88 -0.7 449,681 + -0.2 449,686 - -1.3 449,686 - -2.7 449,690 + +0.6 449,711 + +0.0 449,729 + -0.0 449,756 + -0.1 449,773 - -0.7 449,773 - +0.4 449,776 + -0.3 449,783 - +0.4 449,785 + -0.2 449,785 + -0.8 449,817 + -0.0 449,830 + -1.7 449,848 - -1.3 449,882 + +1.0 449,882 + +0.9 449,892 - +0.7 449,892 - +0.0 449,893 + -1.6 449,893 + -0.6 449,893 + -0.5 449,908 + -1.6 449,908 + -0.5 449,908 + -0.6 449,908 + -1.8 449,916 - -2.3 449,975 + -0.8 449,976 - -0.2 449,977 - -0.3 449,977 - -2.0 449,983 - cyoA b0432 0.10 +1.0 449,983 - cyoA b0432 0.10 -0.4 449,983 - cyoA b0432 0.10 -1.3 449,987 + cyoA b0432 0.11 -1.6 450,027 - cyoA b0432 0.15 +0.4 450,047 + cyoA b0432 0.17 +1.0 450,112 - cyoA b0432 0.24 +0.1 450,178 - cyoA b0432 0.31 -0.9 450,201 - cyoA b0432 0.33 -1.2 450,221 - cyoA b0432 0.35 +0.4 450,221 - cyoA b0432 0.35 -1.0 450,344 + cyoA b0432 0.48 +1.0 450,344 + cyoA b0432 0.48 -1.0 450,353 + cyoA b0432 0.49 -0.8 450,359 - cyoA b0432 0.50 -1.9 450,367 - cyoA b0432 0.51 -0.8 450,367 - cyoA b0432 0.51 -0.3 450,397 + cyoA b0432 0.54 -0.2 450,423 - cyoA b0432 0.57 -0.2 450,432 - cyoA b0432 0.57 -1.5 450,452 - cyoA b0432 0.60 -0.5 450,452 - cyoA b0432 0.60 -1.9 450,463 + cyoA b0432 0.61 -0.5 450,497 - cyoA b0432 0.64 -0.3 450,514 + cyoA b0432 0.66 -0.3 450,522 + cyoA b0432 0.67 -0.4 450,546 - cyoA b0432 0.70 -1.0 450,553 + cyoA b0432 0.70 -0.3 450,562 - cyoA b0432 0.71 +0.4 450,562 - cyoA b0432 0.71 -1.0 450,575 + cyoA b0432 0.73 -2.3 450,581 - cyoA b0432 0.73 -0.6 450,597 + cyoA b0432 0.75 -0.3 450,601 + cyoA b0432 0.75 -0.3 450,601 - cyoA b0432 0.75 +2.0 450,673 - cyoA b0432 0.83 +0.3 450,687 + cyoA b0432 0.84 -1.3 450,702 + cyoA b0432 0.86 +1.0 450,707 + cyoA b0432 0.86 +0.2 450,710 - cyoA b0432 0.87 -1.2 450,715 - cyoA b0432 0.87 -0.2 450,718 + cyoA b0432 0.88 -0.5 450,725 - cyoA b0432 0.88 -0.5 450,730 - cyoA b0432 0.89 -0.3 450,730 - cyoA b0432 0.89 +1.0 450,753 - -0.5 450,763 - -0.4 450,769 + +1.4 450,769 + -0.6 450,774 + -0.9 450,777 - +2.4 450,779 - +0.2 450,779 - +2.0 450,782 - -1.9 450,782 - -1.0 450,792 + -0.3 450,863 - -1.0 450,863 - -1.2
Or see this region's nucleotide sequence