Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF875 and GFF876 are separated by 56 nucleotides GFF876 and GFF877 are separated by 53 nucleotides GFF877 and GFF878 overlap by 4 nucleotides
GFF875 - Uncharacterized protein YtfN, at 51,132 to 54,911
GFF875
GFF876 - UPF0131 protein YtfP, at 54,968 to 55,258
GFF876
GFF877 - Putative sugar transporter, at 55,312 to 56,520
GFF877
GFF878 - FIG01045393: hypothetical protein, at 56,517 to 57,680
GFF878
Position (kb) 55
56
57 Strain fitness (log2 ratio) -1
0
1 at 54.364 kb on - strand, within GFF875 at 54.568 kb on - strand at 54.695 kb on - strand at 54.761 kb on - strand at 54.761 kb on - strand at 54.823 kb on + strand at 54.842 kb on + strand at 54.842 kb on + strand at 54.848 kb on - strand at 54.909 kb on + strand at 54.929 kb on + strand at 54.930 kb on - strand at 55.010 kb on + strand, within GFF876 at 55.011 kb on - strand, within GFF876 at 55.011 kb on - strand, within GFF876 at 55.019 kb on + strand, within GFF876 at 55.020 kb on - strand, within GFF876 at 55.066 kb on - strand, within GFF876 at 55.067 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.184 kb on + strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.185 kb on - strand, within GFF876 at 55.188 kb on + strand, within GFF876 at 55.188 kb on + strand, within GFF876 at 55.188 kb on + strand, within GFF876 at 55.189 kb on - strand, within GFF876 at 55.189 kb on - strand, within GFF876 at 55.189 kb on - strand, within GFF876 at 55.239 kb on + strand at 55.337 kb on - strand at 55.396 kb on - strand at 55.409 kb on + strand at 55.409 kb on + strand at 55.410 kb on - strand at 55.549 kb on - strand, within GFF877 at 55.658 kb on - strand, within GFF877 at 55.678 kb on - strand, within GFF877 at 55.822 kb on - strand, within GFF877 at 55.854 kb on - strand, within GFF877 at 55.870 kb on + strand, within GFF877 at 55.902 kb on + strand, within GFF877 at 55.941 kb on - strand, within GFF877 at 56.020 kb on - strand, within GFF877 at 56.020 kb on - strand, within GFF877 at 56.100 kb on - strand, within GFF877 at 56.223 kb on + strand, within GFF877 at 56.228 kb on + strand, within GFF877 at 56.775 kb on - strand, within GFF878 at 56.794 kb on - strand, within GFF878 at 56.844 kb on - strand, within GFF878 at 56.952 kb on + strand, within GFF878 at 57.008 kb on + strand, within GFF878 at 57.112 kb on - strand, within GFF878 at 57.442 kb on + strand, within GFF878 at 57.443 kb on - strand, within GFF878 at 57.443 kb on - strand, within GFF878 at 57.504 kb on - strand, within GFF878
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 54,364 - GFF875 0.86 +0.2 54,568 - +0.4 54,695 - -1.4 54,761 - -0.7 54,761 - -1.3 54,823 + +0.2 54,842 + +0.9 54,842 + -0.2 54,848 - -0.1 54,909 + -1.3 54,929 + -0.1 54,930 - -0.8 55,010 + GFF876 0.14 -0.8 55,011 - GFF876 0.15 -0.2 55,011 - GFF876 0.15 -0.6 55,019 + GFF876 0.18 -0.4 55,020 - GFF876 0.18 -1.0 55,066 - GFF876 0.34 -0.0 55,067 + GFF876 0.34 +0.5 55,184 + GFF876 0.74 -0.1 55,184 + GFF876 0.74 -0.7 55,184 + GFF876 0.74 -1.1 55,184 + GFF876 0.74 -0.5 55,184 + GFF876 0.74 -0.0 55,184 + GFF876 0.74 -0.8 55,184 + GFF876 0.74 -0.8 55,184 + GFF876 0.74 -0.3 55,185 - GFF876 0.75 -0.8 55,185 - GFF876 0.75 -0.1 55,185 - GFF876 0.75 -0.8 55,185 - GFF876 0.75 -1.6 55,185 - GFF876 0.75 -1.3 55,185 - GFF876 0.75 +0.4 55,185 - GFF876 0.75 -0.5 55,188 + GFF876 0.76 -1.2 55,188 + GFF876 0.76 -0.9 55,188 + GFF876 0.76 -1.4 55,189 - GFF876 0.76 -0.0 55,189 - GFF876 0.76 -1.2 55,189 - GFF876 0.76 -0.8 55,239 + -0.5 55,337 - -0.0 55,396 - -0.3 55,409 + -0.2 55,409 + -0.0 55,410 - +0.3 55,549 - GFF877 0.20 +0.1 55,658 - GFF877 0.29 +0.3 55,678 - GFF877 0.30 -0.2 55,822 - GFF877 0.42 -0.4 55,854 - GFF877 0.45 +0.1 55,870 + GFF877 0.46 -0.5 55,902 + GFF877 0.49 +1.3 55,941 - GFF877 0.52 -0.3 56,020 - GFF877 0.59 -0.6 56,020 - GFF877 0.59 +0.5 56,100 - GFF877 0.65 -0.9 56,223 + GFF877 0.75 -1.0 56,228 + GFF877 0.76 -0.6 56,775 - GFF878 0.22 -0.2 56,794 - GFF878 0.24 -1.0 56,844 - GFF878 0.28 -0.2 56,952 + GFF878 0.37 -0.6 57,008 + GFF878 0.42 -0.2 57,112 - GFF878 0.51 +0.5 57,442 + GFF878 0.79 +0.6 57,443 - GFF878 0.80 -1.2 57,443 - GFF878 0.80 +0.3 57,504 - GFF878 0.85 -0.1
Or see this region's nucleotide sequence