Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4817

Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4815 and GFF4816 are separated by 98 nucleotidesGFF4816 and GFF4817 are separated by 82 nucleotidesGFF4817 and GFF4818 are separated by 87 nucleotidesGFF4818 and GFF4819 are separated by 75 nucleotides GFF4815 - CRISPR-associated helicase Cas3, at 114,006 to 116,669 GFF4815 GFF4816 - hypothetical protein, at 116,768 to 116,902 GFF4816 GFF4817 - Secreted protein, at 116,985 to 118,067 GFF4817 GFF4818 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8), at 118,155 to 118,889 GFF4818 GFF4819 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 118,965 to 120,677 GFF4819 Position (kb) 116 117 118 119Strain fitness (log2 ratio) -1 0 1at 116.539 kb on + strandat 116.654 kb on - strandat 117.735 kb on - strand, within GFF4817at 117.871 kb on + strand, within GFF4817at 117.879 kb on - strand, within GFF4817at 117.999 kb on + strandat 118.131 kb on + strandat 118.131 kb on + strandat 118.139 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
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116,539 + +0.0
116,654 - -0.4
117,735 - GFF4817 0.69 +0.7
117,871 + GFF4817 0.82 +0.8
117,879 - GFF4817 0.83 -0.6
117,999 + -0.3
118,131 + -0.5
118,131 + +0.3
118,139 + -0.1

Or see this region's nucleotide sequence