Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4554

Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4552 and GFF4553 overlap by 26 nucleotidesGFF4553 and GFF4554 overlap by 8 nucleotidesGFF4554 and GFF4555 are separated by 8 nucleotidesGFF4555 and GFF4556 are separated by 14 nucleotides GFF4552 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 335,991 to 337,085 GFF4552 GFF4553 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 337,060 to 337,710 GFF4553 GFF4554 - DNA polymerase III delta subunit (EC 2.7.7.7), at 337,703 to 338,725 GFF4554 GFF4555 - LPS-assembly lipoprotein RlpB precursor (Rare lipoprotein B), at 338,734 to 339,324 GFF4555 GFF4556 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 339,339 to 341,921 GFF4556 Position (kb) 337 338 339Strain fitness (log2 ratio) -1 0 1at 336.885 kb on + strand, within GFF4552at 336.885 kb on + strand, within GFF4552at 336.959 kb on + strand, within GFF4552at 336.973 kb on - strand, within GFF4552at 336.996 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
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336,885 + GFF4552 0.82 -0.4
336,885 + GFF4552 0.82 +0.4
336,959 + GFF4552 0.88 +0.1
336,973 - GFF4552 0.90 -0.2
336,996 + -0.5

Or see this region's nucleotide sequence