Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4400 and GFF4401 are separated by 116 nucleotides GFF4401 and GFF4402 are separated by 201 nucleotides
GFF4400 - 'UDP-sugar hydrolase (EC 3.6.1.45); 5'-nucleotidase (EC 3.1.3.5)' transl_table=11, at 183,848 to 185,500
GFF4400
GFF4401 - Cys-tRNA(Pro) deacylase YbaK, at 185,617 to 186,096
GFF4401
GFF4402 - FIG00921183: possible ligase, at 186,298 to 187,092
GFF4402
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1
2 at 184.652 kb on + strand, within GFF4400 at 184.652 kb on + strand, within GFF4400 at 184.657 kb on - strand, within GFF4400 at 184.724 kb on - strand, within GFF4400 at 184.728 kb on - strand, within GFF4400 at 184.728 kb on - strand, within GFF4400 at 184.728 kb on - strand, within GFF4400 at 184.845 kb on + strand, within GFF4400 at 184.999 kb on + strand, within GFF4400 at 185.098 kb on + strand, within GFF4400 at 185.099 kb on - strand, within GFF4400 at 185.099 kb on - strand, within GFF4400 at 185.101 kb on + strand, within GFF4400 at 185.102 kb on - strand, within GFF4400 at 185.106 kb on - strand, within GFF4400 at 185.107 kb on + strand, within GFF4400 at 185.151 kb on - strand, within GFF4400 at 185.151 kb on - strand, within GFF4400 at 185.183 kb on + strand, within GFF4400 at 185.225 kb on + strand, within GFF4400 at 185.407 kb on + strand at 185.608 kb on + strand at 185.704 kb on - strand, within GFF4401 at 185.718 kb on - strand, within GFF4401 at 185.721 kb on + strand, within GFF4401 at 185.722 kb on - strand, within GFF4401 at 185.964 kb on - strand, within GFF4401 at 185.992 kb on - strand, within GFF4401 at 185.992 kb on - strand, within GFF4401 at 185.994 kb on - strand, within GFF4401 at 186.110 kb on - strand at 186.112 kb on + strand at 186.113 kb on - strand at 186.249 kb on - strand at 186.362 kb on + strand at 186.425 kb on - strand, within GFF4402 at 186.444 kb on + strand, within GFF4402 at 186.659 kb on - strand, within GFF4402 at 186.661 kb on + strand, within GFF4402 at 186.662 kb on - strand, within GFF4402 at 186.668 kb on + strand, within GFF4402 at 186.721 kb on + strand, within GFF4402 at 186.729 kb on + strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.730 kb on - strand, within GFF4402 at 186.733 kb on + strand, within GFF4402 at 186.734 kb on - strand, within GFF4402 at 186.734 kb on - strand, within GFF4402 at 186.774 kb on + strand, within GFF4402 at 187.023 kb on + strand at 187.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 184,652 + GFF4400 0.49 -0.1 184,652 + GFF4400 0.49 +0.0 184,657 - GFF4400 0.49 -0.0 184,724 - GFF4400 0.53 -0.3 184,728 - GFF4400 0.53 +0.2 184,728 - GFF4400 0.53 +0.1 184,728 - GFF4400 0.53 +0.2 184,845 + GFF4400 0.60 -0.5 184,999 + GFF4400 0.70 -0.9 185,098 + GFF4400 0.76 +0.0 185,099 - GFF4400 0.76 -0.2 185,099 - GFF4400 0.76 -0.4 185,101 + GFF4400 0.76 -0.2 185,102 - GFF4400 0.76 -0.6 185,106 - GFF4400 0.76 -0.3 185,107 + GFF4400 0.76 +0.3 185,151 - GFF4400 0.79 +0.1 185,151 - GFF4400 0.79 +0.9 185,183 + GFF4400 0.81 +0.8 185,225 + GFF4400 0.83 -0.4 185,407 + -0.0 185,608 + -0.4 185,704 - GFF4401 0.18 -0.6 185,718 - GFF4401 0.21 -0.3 185,721 + GFF4401 0.22 -0.1 185,722 - GFF4401 0.22 +0.5 185,964 - GFF4401 0.72 -0.0 185,992 - GFF4401 0.78 -2.1 185,992 - GFF4401 0.78 +0.1 185,994 - GFF4401 0.79 -0.4 186,110 - -1.7 186,112 + +0.3 186,113 - +1.2 186,249 - +0.4 186,362 + -1.0 186,425 - GFF4402 0.16 +0.8 186,444 + GFF4402 0.18 -0.2 186,659 - GFF4402 0.45 +1.0 186,661 + GFF4402 0.46 +0.9 186,662 - GFF4402 0.46 -0.0 186,668 + GFF4402 0.47 +0.5 186,721 + GFF4402 0.53 +0.1 186,729 + GFF4402 0.54 +0.0 186,730 - GFF4402 0.54 +0.1 186,730 - GFF4402 0.54 +2.0 186,730 - GFF4402 0.54 -0.0 186,733 + GFF4402 0.55 -0.5 186,734 - GFF4402 0.55 -0.0 186,734 - GFF4402 0.55 -0.0 186,774 + GFF4402 0.60 -0.0 187,023 + +0.3 187,082 - +0.6
Or see this region's nucleotide sequence