Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4130 and GFF4131 are separated by 222 nucleotides GFF4131 and GFF4132 are separated by 129 nucleotides GFF4132 and GFF4133 overlap by 4 nucleotides
GFF4130 - putative dipeptide/oligopeptide/nickel ABC-type transport system periplasmic component, at 11,867 to 13,546
GFF4130
GFF4131 - PTS system, glucose-specific IIC component (EC 2.7.1.69) / PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIA component (EC 2.7.1.69), at 13,769 to 15,310
GFF4131
GFF4132 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 15,440 to 16,282
GFF4132
GFF4133 - 6-phospho-3-hexuloisomerase, at 16,279 to 16,845
GFF4133
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.774 kb on + strand, within GFF4130 at 12.845 kb on + strand, within GFF4130 at 13.170 kb on + strand, within GFF4130 at 13.201 kb on + strand, within GFF4130 at 13.245 kb on + strand, within GFF4130 at 13.541 kb on - strand at 13.546 kb on + strand at 13.745 kb on + strand at 13.797 kb on + strand at 13.798 kb on - strand at 14.072 kb on - strand, within GFF4131 at 14.136 kb on - strand, within GFF4131 at 14.219 kb on - strand, within GFF4131 at 14.536 kb on + strand, within GFF4131 at 14.689 kb on + strand, within GFF4131 at 14.690 kb on - strand, within GFF4131 at 14.864 kb on - strand, within GFF4131 at 14.870 kb on + strand, within GFF4131 at 14.909 kb on + strand, within GFF4131 at 14.909 kb on + strand, within GFF4131 at 14.909 kb on + strand, within GFF4131 at 14.951 kb on + strand, within GFF4131 at 14.951 kb on + strand, within GFF4131 at 14.951 kb on + strand, within GFF4131 at 15.091 kb on + strand, within GFF4131 at 15.092 kb on - strand, within GFF4131 at 15.092 kb on - strand, within GFF4131 at 15.143 kb on - strand, within GFF4131 at 15.359 kb on + strand at 15.363 kb on + strand at 15.363 kb on + strand at 15.364 kb on - strand at 15.367 kb on + strand at 15.367 kb on + strand at 15.368 kb on - strand at 15.399 kb on + strand at 15.400 kb on - strand at 15.416 kb on - strand at 15.416 kb on - strand at 15.424 kb on + strand at 15.425 kb on - strand at 15.426 kb on + strand at 15.427 kb on - strand at 15.427 kb on - strand at 15.603 kb on - strand, within GFF4132 at 15.662 kb on + strand, within GFF4132 at 15.776 kb on + strand, within GFF4132 at 15.776 kb on + strand, within GFF4132 at 15.783 kb on + strand, within GFF4132 at 15.783 kb on + strand, within GFF4132 at 15.787 kb on + strand, within GFF4132 at 15.788 kb on - strand, within GFF4132 at 15.803 kb on + strand, within GFF4132 at 15.804 kb on - strand, within GFF4132 at 15.804 kb on - strand, within GFF4132 at 15.898 kb on + strand, within GFF4132 at 15.899 kb on - strand, within GFF4132 at 15.983 kb on - strand, within GFF4132 at 15.993 kb on - strand, within GFF4132 at 16.010 kb on + strand, within GFF4132 at 16.144 kb on + strand, within GFF4132 at 16.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 12,774 + GFF4130 0.54 +0.5 12,845 + GFF4130 0.58 +0.5 13,170 + GFF4130 0.78 +0.1 13,201 + GFF4130 0.79 +0.7 13,245 + GFF4130 0.82 -1.8 13,541 - -0.2 13,546 + -0.7 13,745 + +0.1 13,797 + -0.0 13,798 - +0.2 14,072 - GFF4131 0.20 +0.3 14,136 - GFF4131 0.24 +1.5 14,219 - GFF4131 0.29 +0.1 14,536 + GFF4131 0.50 +0.0 14,689 + GFF4131 0.60 +2.4 14,690 - GFF4131 0.60 +0.7 14,864 - GFF4131 0.71 -0.7 14,870 + GFF4131 0.71 -0.0 14,909 + GFF4131 0.74 +0.3 14,909 + GFF4131 0.74 -0.0 14,909 + GFF4131 0.74 +0.2 14,951 + GFF4131 0.77 +0.0 14,951 + GFF4131 0.77 -0.2 14,951 + GFF4131 0.77 +0.3 15,091 + GFF4131 0.86 -0.4 15,092 - GFF4131 0.86 +0.3 15,092 - GFF4131 0.86 -0.1 15,143 - GFF4131 0.89 +0.5 15,359 + +0.2 15,363 + +0.2 15,363 + -0.0 15,364 - +0.9 15,367 + -1.3 15,367 + +0.0 15,368 - +0.0 15,399 + +0.1 15,400 - +0.3 15,416 - -0.6 15,416 - +0.1 15,424 + +0.6 15,425 - +0.0 15,426 + +0.5 15,427 - +0.1 15,427 - +0.4 15,603 - GFF4132 0.19 +0.3 15,662 + GFF4132 0.26 -0.2 15,776 + GFF4132 0.40 -0.3 15,776 + GFF4132 0.40 -0.6 15,783 + GFF4132 0.41 +0.4 15,783 + GFF4132 0.41 +0.4 15,787 + GFF4132 0.41 -1.1 15,788 - GFF4132 0.41 +0.4 15,803 + GFF4132 0.43 +0.1 15,804 - GFF4132 0.43 -0.4 15,804 - GFF4132 0.43 -0.3 15,898 + GFF4132 0.54 -2.0 15,899 - GFF4132 0.54 +1.0 15,983 - GFF4132 0.64 -0.9 15,993 - GFF4132 0.66 -0.3 16,010 + GFF4132 0.68 +0.1 16,144 + GFF4132 0.84 +0.4 16,211 + +0.5
Or see this region's nucleotide sequence