Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3464

Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3461 and GFF3462 are separated by 254 nucleotidesGFF3462 and GFF3463 are separated by 68 nucleotidesGFF3463 and GFF3464 overlap by 15 nucleotidesGFF3464 and GFF3465 are separated by 118 nucleotides GFF3461 - hypothetical protein, at 215,501 to 215,617 GFF3461 GFF3462 - FIG00638802: hypothetical protein, at 215,872 to 216,123 GFF3462 GFF3463 - FIG00638667: hypothetical protein, at 216,192 to 216,503 GFF3463 GFF3464 - FIG00638254: hypothetical protein, at 216,489 to 216,836 GFF3464 GFF3465 - hypothetical protein, at 216,955 to 218,139 GFF3465 Position (kb) 216 217Strain fitness (log2 ratio) -1 0 1 2at 215.537 kb on + strand, within GFF3461at 215.680 kb on - strandat 215.683 kb on - strandat 215.711 kb on - strandat 215.860 kb on + strandat 215.903 kb on + strand, within GFF3462at 216.150 kb on + strandat 216.223 kb on + strandat 216.281 kb on - strand, within GFF3463at 216.539 kb on + strand, within GFF3464at 216.553 kb on + strand, within GFF3464at 216.727 kb on + strand, within GFF3464at 216.727 kb on + strand, within GFF3464at 216.953 kb on - strandat 216.953 kb on - strandat 216.956 kb on + strandat 217.040 kb on - strandat 217.223 kb on - strand, within GFF3465at 217.429 kb on + strand, within GFF3465at 217.548 kb on + strand, within GFF3465at 217.548 kb on + strand, within GFF3465

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
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215,537 + GFF3461 0.31 -1.0
215,680 - +0.2
215,683 - -0.5
215,711 - -0.5
215,860 + -1.0
215,903 + GFF3462 0.12 -0.3
216,150 + +0.2
216,223 + -0.8
216,281 - GFF3463 0.29 +0.4
216,539 + GFF3464 0.14 -0.2
216,553 + GFF3464 0.18 -1.5
216,727 + GFF3464 0.68 -0.2
216,727 + GFF3464 0.68 +0.0
216,953 - -0.2
216,953 - -0.1
216,956 + +0.3
217,040 - +1.8
217,223 - GFF3465 0.23 +0.7
217,429 + GFF3465 0.40 +0.0
217,548 + GFF3465 0.50 +0.2
217,548 + GFF3465 0.50 -0.0

Or see this region's nucleotide sequence