Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3193 and GFF3194 are separated by 144 nucleotides GFF3194 and GFF3195 are separated by 41 nucleotides GFF3195 and GFF3196 are separated by 2 nucleotides GFF3196 and GFF3197 overlap by 4 nucleotides GFF3197 and GFF3198 are separated by 144 nucleotides GFF3198 and GFF3199 are separated by 67 nucleotides
GFF3193 - Uncharacterized protein YqjB, at 200,995 to 201,378
GFF3193
GFF3194 - Periplasmic protein YqjC, at 201,523 to 201,891
GFF3194
GFF3195 - Uncharacterized membrane protein YqjD, at 201,933 to 202,238
GFF3195
GFF3196 - Inner membrane protein YqjE, at 202,241 to 202,639
GFF3196
GFF3197 - Inner membrane protein YqjK, at 202,636 to 202,935
GFF3197
GFF3198 - Inner membrane protein YqjF, at 203,080 to 203,565
GFF3198
GFF3199 - Glutathione S-transferase, omega (EC 2.5.1.18), at 203,633 to 204,619
GFF3199
Position (kb) 202
203 Strain fitness (log2 ratio) -1
0
1 at 201.284 kb on + strand, within GFF3193 at 201.296 kb on - strand, within GFF3193 at 201.439 kb on + strand at 201.443 kb on - strand at 201.443 kb on - strand at 201.565 kb on - strand, within GFF3194 at 201.578 kb on - strand, within GFF3194 at 201.583 kb on - strand, within GFF3194 at 201.583 kb on - strand, within GFF3194 at 201.614 kb on + strand, within GFF3194 at 201.614 kb on + strand, within GFF3194 at 201.627 kb on + strand, within GFF3194 at 201.837 kb on + strand, within GFF3194 at 202.155 kb on + strand, within GFF3195 at 202.220 kb on - strand at 202.350 kb on + strand, within GFF3196 at 202.767 kb on + strand, within GFF3197 at 202.775 kb on + strand, within GFF3197 at 202.812 kb on - strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 203.281 kb on + strand, within GFF3198 at 203.281 kb on + strand, within GFF3198 at 203.282 kb on - strand, within GFF3198 at 203.327 kb on + strand, within GFF3198 at 203.406 kb on + strand, within GFF3198 at 203.453 kb on + strand, within GFF3198 at 203.454 kb on - strand, within GFF3198 at 203.504 kb on + strand, within GFF3198 at 203.573 kb on - strand at 203.574 kb on + strand at 203.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 201,284 + GFF3193 0.75 +0.2 201,296 - GFF3193 0.78 -0.0 201,439 + +0.2 201,443 - +0.4 201,443 - -0.5 201,565 - GFF3194 0.11 +0.7 201,578 - GFF3194 0.15 -0.2 201,583 - GFF3194 0.16 -0.0 201,583 - GFF3194 0.16 +0.1 201,614 + GFF3194 0.25 -0.2 201,614 + GFF3194 0.25 -0.4 201,627 + GFF3194 0.28 +0.1 201,837 + GFF3194 0.85 +0.3 202,155 + GFF3195 0.73 +0.9 202,220 - -0.3 202,350 + GFF3196 0.27 +0.2 202,767 + GFF3197 0.44 +0.3 202,775 + GFF3197 0.46 -0.7 202,812 - GFF3197 0.59 -0.5 202,842 + GFF3197 0.69 -0.1 202,842 + GFF3197 0.69 +0.0 202,842 + GFF3197 0.69 -0.4 202,843 - GFF3197 0.69 -0.1 202,843 - GFF3197 0.69 -0.2 202,843 - GFF3197 0.69 -0.1 202,843 - GFF3197 0.69 -0.1 203,281 + GFF3198 0.41 -0.1 203,281 + GFF3198 0.41 -0.4 203,282 - GFF3198 0.42 -0.3 203,327 + GFF3198 0.51 -1.1 203,406 + GFF3198 0.67 -0.2 203,453 + GFF3198 0.77 -0.3 203,454 - GFF3198 0.77 -0.2 203,504 + GFF3198 0.87 +0.3 203,573 - +0.2 203,574 + -0.2 203,583 - -0.3
Or see this region's nucleotide sequence