Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2794 and GFF2795 are separated by 496 nucleotides GFF2795 and GFF2796 are separated by 17 nucleotides GFF2796 and GFF2797 are separated by 79 nucleotides
GFF2794 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 317,218 to 318,354
GFF2794
GFF2795 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 318,851 to 320,125
GFF2795
GFF2796 - hypothetical protein, at 320,143 to 320,259
GFF2796
GFF2797 - Trehalase (EC 3.2.1.28); Periplasmic trehalase precursor (EC 3.2.1.28), at 320,339 to 322,051
GFF2797
Position (kb) 318
319
320
321 Strain fitness (log2 ratio) -1
0
1 at 317.994 kb on - strand, within GFF2794 at 318.171 kb on + strand, within GFF2794 at 318.274 kb on + strand at 318.275 kb on - strand at 318.276 kb on + strand at 318.463 kb on - strand at 318.557 kb on + strand at 318.796 kb on + strand at 318.803 kb on + strand at 318.826 kb on - strand at 318.866 kb on + strand at 318.898 kb on + strand at 318.898 kb on + strand at 319.023 kb on + strand, within GFF2795 at 319.253 kb on + strand, within GFF2795 at 319.274 kb on - strand, within GFF2795 at 319.294 kb on + strand, within GFF2795 at 319.324 kb on + strand, within GFF2795 at 319.352 kb on + strand, within GFF2795 at 319.352 kb on + strand, within GFF2795 at 319.403 kb on - strand, within GFF2795 at 319.403 kb on - strand, within GFF2795 at 319.489 kb on + strand, within GFF2795 at 319.600 kb on + strand, within GFF2795 at 319.615 kb on + strand, within GFF2795 at 319.700 kb on - strand, within GFF2795 at 319.799 kb on - strand, within GFF2795 at 319.868 kb on - strand, within GFF2795 at 319.939 kb on + strand, within GFF2795 at 320.065 kb on + strand at 320.065 kb on + strand at 320.115 kb on - strand at 320.145 kb on - strand at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.178 kb on + strand, within GFF2796 at 320.222 kb on - strand, within GFF2796 at 320.264 kb on - strand at 320.298 kb on - strand at 320.504 kb on - strand at 320.564 kb on - strand, within GFF2797 at 320.713 kb on + strand, within GFF2797 at 320.795 kb on + strand, within GFF2797 at 320.938 kb on + strand, within GFF2797 at 320.957 kb on - strand, within GFF2797 at 320.957 kb on - strand, within GFF2797 at 320.960 kb on + strand, within GFF2797
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 317,994 - GFF2794 0.68 +0.3 318,171 + GFF2794 0.84 +0.2 318,274 + -1.7 318,275 - +0.5 318,276 + +0.9 318,463 - +0.3 318,557 + -1.3 318,796 + +0.2 318,803 + +0.4 318,826 - -0.4 318,866 + -0.0 318,898 + -0.2 318,898 + +0.0 319,023 + GFF2795 0.13 -0.1 319,253 + GFF2795 0.32 -0.3 319,274 - GFF2795 0.33 +0.5 319,294 + GFF2795 0.35 +0.0 319,324 + GFF2795 0.37 -0.9 319,352 + GFF2795 0.39 +0.1 319,352 + GFF2795 0.39 +0.3 319,403 - GFF2795 0.43 -0.0 319,403 - GFF2795 0.43 -1.3 319,489 + GFF2795 0.50 -0.3 319,600 + GFF2795 0.59 -0.3 319,615 + GFF2795 0.60 +0.3 319,700 - GFF2795 0.67 +0.2 319,799 - GFF2795 0.74 +0.1 319,868 - GFF2795 0.80 -0.4 319,939 + GFF2795 0.85 -0.2 320,065 + -0.5 320,065 + -0.1 320,115 - +0.1 320,145 - +0.2 320,178 + GFF2796 0.30 -1.0 320,178 + GFF2796 0.30 -0.2 320,178 + GFF2796 0.30 +0.9 320,178 + GFF2796 0.30 -0.5 320,222 - GFF2796 0.68 +0.5 320,264 - -1.5 320,298 - -1.4 320,504 - -0.9 320,564 - GFF2797 0.13 +0.5 320,713 + GFF2797 0.22 +0.2 320,795 + GFF2797 0.27 -0.3 320,938 + GFF2797 0.35 +0.0 320,957 - GFF2797 0.36 +0.8 320,957 - GFF2797 0.36 -0.9 320,960 + GFF2797 0.36 -0.2
Or see this region's nucleotide sequence