Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF252 and GFF253 are separated by 867 nucleotides GFF253 and GFF254 are separated by 248 nucleotides
GFF252 - Cof protein, HD superfamily hydrolase, at 25,726 to 26,526
GFF252
GFF253 - Transcriptional activator MetR, at 27,394 to 28,347
GFF253
GFF254 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 28,596 to 30,860
GFF254
Position (kb) 27
28
29 Strain fitness (log2 ratio) -1
0
1
2 at 26.520 kb on - strand at 26.541 kb on - strand at 26.572 kb on - strand at 26.593 kb on - strand at 26.624 kb on + strand at 26.625 kb on - strand at 26.630 kb on + strand at 26.657 kb on - strand at 26.687 kb on + strand at 26.691 kb on + strand at 26.692 kb on - strand at 26.692 kb on - strand at 26.692 kb on - strand at 26.768 kb on - strand at 26.784 kb on + strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.786 kb on + strand at 26.787 kb on - strand at 26.820 kb on + strand at 26.820 kb on + strand at 26.821 kb on - strand at 26.821 kb on - strand at 26.821 kb on - strand at 26.823 kb on - strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.842 kb on - strand at 26.895 kb on + strand at 26.897 kb on + strand at 26.911 kb on - strand at 26.955 kb on - strand at 27.047 kb on + strand at 27.048 kb on - strand at 27.048 kb on - strand at 27.050 kb on + strand at 27.079 kb on - strand at 27.262 kb on - strand at 27.266 kb on + strand at 27.267 kb on - strand at 27.276 kb on + strand at 27.312 kb on - strand at 27.320 kb on + strand at 27.321 kb on + strand at 27.396 kb on - strand at 27.811 kb on - strand, within GFF253 at 27.885 kb on - strand, within GFF253 at 28.048 kb on - strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.065 kb on + strand, within GFF253 at 28.336 kb on - strand at 28.390 kb on + strand at 28.390 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.397 kb on + strand at 28.403 kb on + strand at 28.447 kb on - strand at 28.494 kb on - strand at 28.494 kb on - strand at 28.671 kb on + strand at 28.772 kb on - strand at 28.956 kb on - strand, within GFF254 at 28.958 kb on + strand, within GFF254 at 28.958 kb on + strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.962 kb on + strand, within GFF254 at 28.963 kb on - strand, within GFF254 at 28.963 kb on - strand, within GFF254 at 28.968 kb on + strand, within GFF254 at 28.969 kb on + strand, within GFF254 at 28.969 kb on - strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 29.074 kb on + strand, within GFF254 at 29.074 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.166 kb on + strand, within GFF254 at 29.176 kb on + strand, within GFF254 at 29.176 kb on + strand, within GFF254 at 29.201 kb on + strand, within GFF254 at 29.201 kb on + strand, within GFF254 at 29.322 kb on + strand, within GFF254
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 26,520 - -0.5 26,541 - -0.1 26,572 - +0.3 26,593 - -0.5 26,624 + -0.2 26,625 - +0.2 26,630 + +0.0 26,657 - +0.4 26,687 + +0.2 26,691 + +0.7 26,692 - +0.4 26,692 - +0.3 26,692 - +0.1 26,768 - -0.0 26,784 + -0.4 26,785 - -0.0 26,785 - +0.4 26,786 + -0.1 26,787 - +0.9 26,820 + +1.2 26,820 + +0.4 26,821 - -0.3 26,821 - -0.2 26,821 - +0.3 26,823 - +1.3 26,841 + +0.2 26,841 + +0.2 26,841 + +0.3 26,841 + +0.3 26,842 - +0.3 26,895 + +1.7 26,897 + +0.3 26,911 - -0.7 26,955 - +0.7 27,047 + +0.7 27,048 - -0.4 27,048 - -0.0 27,050 + +0.6 27,079 - -0.1 27,262 - -0.1 27,266 + +0.9 27,267 - +0.4 27,276 + +0.1 27,312 - -0.1 27,320 + +0.3 27,321 + -0.3 27,396 - +0.2 27,811 - GFF253 0.44 -0.1 27,885 - GFF253 0.51 +0.3 28,048 - GFF253 0.69 +0.2 28,053 + GFF253 0.69 -0.1 28,053 + GFF253 0.69 -0.1 28,053 + GFF253 0.69 -1.3 28,065 + GFF253 0.70 -0.7 28,336 - +1.0 28,390 + +0.7 28,390 + +0.2 28,392 + +1.1 28,392 + +1.0 28,392 + -0.1 28,392 + -1.2 28,392 + -0.1 28,392 + +0.2 28,392 + -0.1 28,397 + +0.2 28,403 + +0.8 28,447 - +0.1 28,494 - +0.6 28,494 - -0.1 28,671 + -0.0 28,772 - +0.1 28,956 - GFF254 0.16 -0.3 28,958 + GFF254 0.16 -0.2 28,958 + GFF254 0.16 -0.1 28,959 - GFF254 0.16 -0.7 28,959 - GFF254 0.16 +0.3 28,959 - GFF254 0.16 -0.2 28,959 - GFF254 0.16 +0.1 28,959 - GFF254 0.16 +0.1 28,962 + GFF254 0.16 +1.3 28,963 - GFF254 0.16 +0.4 28,963 - GFF254 0.16 +0.4 28,968 + GFF254 0.16 -0.1 28,969 + GFF254 0.16 -0.9 28,969 - GFF254 0.16 +0.2 28,972 + GFF254 0.17 +0.2 28,972 + GFF254 0.17 +0.5 28,972 + GFF254 0.17 -0.8 28,972 + GFF254 0.17 -0.0 28,972 + GFF254 0.17 -0.3 28,972 + GFF254 0.17 +0.0 28,973 - GFF254 0.17 +2.1 28,973 - GFF254 0.17 -0.2 28,973 - GFF254 0.17 +0.3 29,074 + GFF254 0.21 +0.2 29,074 + GFF254 0.21 +1.5 29,094 + GFF254 0.22 +0.0 29,094 + GFF254 0.22 +0.1 29,094 + GFF254 0.22 +0.3 29,095 - GFF254 0.22 -0.8 29,095 - GFF254 0.22 -0.3 29,095 - GFF254 0.22 +0.0 29,166 + GFF254 0.25 -0.1 29,176 + GFF254 0.26 +0.1 29,176 + GFF254 0.26 +0.7 29,201 + GFF254 0.27 +0.1 29,201 + GFF254 0.27 +0.7 29,322 + GFF254 0.32 +0.2
Or see this region's nucleotide sequence