Experiment: LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1283 and GFF1284 are separated by 10 nucleotides GFF1284 and GFF1285 are separated by 225 nucleotides GFF1285 and GFF1286 are separated by 3 nucleotides GFF1286 and GFF1287 are separated by 48 nucleotides
GFF1283 - Phage capsid and scaffold, at 10,378 to 10,737
GFF1283
GFF1284 - Phage capsid and scaffold, at 10,748 to 11,131
GFF1284
GFF1285 - FIG01049612: hypothetical protein, at 11,357 to 11,560
GFF1285
GFF1286 - Phage tail completion protein, at 11,564 to 11,737
GFF1286
GFF1287 - putative virulence protein, at 11,786 to 13,036
GFF1287
Position (kb)
11
12 Strain fitness (log2 ratio)
-1
0
1 at 10.400 kb on + strand at 10.401 kb on - strand at 10.610 kb on + strand, within GFF1283 at 10.611 kb on - strand, within GFF1283 at 10.660 kb on - strand, within GFF1283 at 10.747 kb on + strand at 10.865 kb on - strand, within GFF1284 at 10.989 kb on + strand, within GFF1284 at 11.050 kb on + strand, within GFF1284 at 11.051 kb on - strand, within GFF1284 at 11.053 kb on + strand, within GFF1284 at 11.054 kb on - strand, within GFF1284 at 11.308 kb on + strand at 11.317 kb on - strand at 11.381 kb on - strand, within GFF1285 at 11.510 kb on + strand, within GFF1285 at 11.562 kb on + strand at 11.564 kb on - strand at 11.564 kb on - strand at 11.723 kb on + strand at 11.858 kb on - strand at 11.860 kb on + strand at 12.071 kb on + strand, within GFF1287 at 12.131 kb on + strand, within GFF1287 at 12.365 kb on + strand, within GFF1287 at 12.544 kb on - strand, within GFF1287
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.000001875 MOI remove 10,400 + +0.7 10,401 - +1.3 10,610 + GFF1283 0.64 -0.3 10,611 - GFF1283 0.65 +0.1 10,660 - GFF1283 0.78 -0.1 10,747 + +0.3 10,865 - GFF1284 0.30 -0.2 10,989 + GFF1284 0.63 -0.3 11,050 + GFF1284 0.79 +0.4 11,051 - GFF1284 0.79 +0.1 11,053 + GFF1284 0.79 +0.8 11,054 - GFF1284 0.80 +0.1 11,308 + -0.3 11,317 - -0.2 11,381 - GFF1285 0.12 -0.6 11,510 + GFF1285 0.75 -0.2 11,562 + +0.3 11,564 - +0.3 11,564 - +0.0 11,723 + +0.1 11,858 - -0.3 11,860 + -0.2 12,071 + GFF1287 0.23 +0.5 12,131 + GFF1287 0.28 -0.3 12,365 + GFF1287 0.46 +0.4 12,544 - GFF1287 0.61 -0.9
Or see this region's nucleotide sequence