Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3072 and Atu3073 are separated by 164 nucleotides Atu3073 and Atu3074 are separated by 205 nucleotides
Atu3072: Atu3072 - peptide synthetase, at 74,687 to 78,721
Atu3072
Atu3073: Atu3073 - aspartate racemase, at 78,886 to 79,578
Atu3073
Atu3074: Atu3074 - short chain dehydrogenase, at 79,784 to 80,557
Atu3074
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1 at 77.892 kb on + strand, within Atu3072 at 77.910 kb on - strand, within Atu3072 at 77.913 kb on + strand, within Atu3072 at 77.913 kb on + strand, within Atu3072 at 77.913 kb on + strand, within Atu3072 at 77.921 kb on - strand, within Atu3072 at 77.952 kb on - strand, within Atu3072 at 77.990 kb on + strand, within Atu3072 at 78.019 kb on - strand, within Atu3072 at 78.051 kb on - strand, within Atu3072 at 78.280 kb on + strand, within Atu3072 at 78.280 kb on + strand, within Atu3072 at 78.296 kb on - strand, within Atu3072 at 78.360 kb on + strand at 78.368 kb on - strand at 78.369 kb on + strand at 78.377 kb on - strand at 78.381 kb on + strand at 78.391 kb on + strand at 78.391 kb on + strand at 78.399 kb on - strand at 78.497 kb on + strand at 78.526 kb on - strand at 78.636 kb on + strand at 78.744 kb on - strand at 78.806 kb on + strand at 78.882 kb on + strand at 78.882 kb on + strand at 78.882 kb on + strand at 78.882 kb on + strand at 78.984 kb on + strand, within Atu3073 at 79.110 kb on + strand, within Atu3073 at 79.118 kb on - strand, within Atu3073 at 79.145 kb on + strand, within Atu3073 at 79.225 kb on + strand, within Atu3073 at 79.225 kb on + strand, within Atu3073 at 79.233 kb on - strand, within Atu3073 at 79.235 kb on + strand, within Atu3073 at 79.323 kb on - strand, within Atu3073 at 79.376 kb on - strand, within Atu3073 at 79.387 kb on + strand, within Atu3073 at 79.459 kb on + strand, within Atu3073 at 79.652 kb on + strand at 79.686 kb on - strand at 79.713 kb on + strand at 79.741 kb on + strand at 79.766 kb on - strand at 79.936 kb on - strand, within Atu3074 at 80.099 kb on + strand, within Atu3074 at 80.147 kb on + strand, within Atu3074 at 80.157 kb on + strand, within Atu3074 at 80.176 kb on - strand, within Atu3074 at 80.312 kb on + strand, within Atu3074 at 80.318 kb on - strand, within Atu3074 at 80.318 kb on - strand, within Atu3074 at 80.388 kb on - strand, within Atu3074 at 80.432 kb on - strand, within Atu3074 at 80.453 kb on + strand, within Atu3074 at 80.453 kb on + strand, within Atu3074 at 80.461 kb on - strand, within Atu3074 at 80.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.02 mM remove 77,892 + Atu3072 0.79 -1.4 77,910 - Atu3072 0.80 -0.2 77,913 + Atu3072 0.80 -1.8 77,913 + Atu3072 0.80 -0.5 77,913 + Atu3072 0.80 -0.9 77,921 - Atu3072 0.80 +0.1 77,952 - Atu3072 0.81 -0.5 77,990 + Atu3072 0.82 -1.3 78,019 - Atu3072 0.83 +0.2 78,051 - Atu3072 0.83 +0.1 78,280 + Atu3072 0.89 -0.3 78,280 + Atu3072 0.89 +0.6 78,296 - Atu3072 0.89 -0.4 78,360 + -0.8 78,368 - -1.0 78,369 + +1.7 78,377 - +0.2 78,381 + -0.7 78,391 + -2.4 78,391 + -0.4 78,399 - -0.3 78,497 + -0.1 78,526 - -1.1 78,636 + -0.5 78,744 - -1.2 78,806 + -0.3 78,882 + +0.1 78,882 + -0.3 78,882 + -1.2 78,882 + +0.3 78,984 + Atu3073 0.14 -0.0 79,110 + Atu3073 0.32 -0.9 79,118 - Atu3073 0.33 +0.0 79,145 + Atu3073 0.37 +1.1 79,225 + Atu3073 0.49 -0.7 79,225 + Atu3073 0.49 -1.3 79,233 - Atu3073 0.50 -0.4 79,235 + Atu3073 0.50 -1.8 79,323 - Atu3073 0.63 -0.7 79,376 - Atu3073 0.71 -0.3 79,387 + Atu3073 0.72 -0.0 79,459 + Atu3073 0.83 -0.4 79,652 + -0.1 79,686 - -1.3 79,713 + +0.3 79,741 + -1.0 79,766 - -0.2 79,936 - Atu3074 0.20 +0.4 80,099 + Atu3074 0.41 -0.6 80,147 + Atu3074 0.47 +0.2 80,157 + Atu3074 0.48 -0.2 80,176 - Atu3074 0.51 -0.3 80,312 + Atu3074 0.68 -2.4 80,318 - Atu3074 0.69 -0.8 80,318 - Atu3074 0.69 -0.4 80,388 - Atu3074 0.78 -1.4 80,432 - Atu3074 0.84 -2.2 80,453 + Atu3074 0.86 -1.0 80,453 + Atu3074 0.86 -0.8 80,461 - Atu3074 0.87 -0.3 80,506 - -2.1
Or see this region's nucleotide sequence