Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3048 and Atu3049 are separated by 129 nucleotides Atu3049 and Atu3050 are separated by 136 nucleotides
Atu3048: Atu3048 - oligopeptide ABC transporter permease, at 44,419 to 45,369
Atu3048
Atu3049: Atu3049 - oligopeptide ABC transporter substrate-binding protein, at 45,499 to 47,160
Atu3049
Atu3050: Atu3050 - AgaE protein, at 47,297 to 48,628
Atu3050
Position (kb)
45
46
47
48 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 44.563 kb on + strand, within Atu3048 at 44.563 kb on + strand, within Atu3048 at 44.568 kb on + strand, within Atu3048 at 44.568 kb on + strand, within Atu3048 at 44.571 kb on - strand, within Atu3048 at 44.578 kb on + strand, within Atu3048 at 44.578 kb on + strand, within Atu3048 at 44.586 kb on - strand, within Atu3048 at 44.586 kb on - strand, within Atu3048 at 44.609 kb on - strand, within Atu3048 at 44.650 kb on + strand, within Atu3048 at 44.655 kb on - strand, within Atu3048 at 44.956 kb on + strand, within Atu3048 at 45.009 kb on + strand, within Atu3048 at 45.017 kb on - strand, within Atu3048 at 45.017 kb on - strand, within Atu3048 at 45.136 kb on - strand, within Atu3048 at 45.190 kb on - strand, within Atu3048 at 45.369 kb on - strand at 45.370 kb on + strand at 45.401 kb on + strand at 45.409 kb on - strand at 45.409 kb on - strand at 45.474 kb on - strand at 45.495 kb on + strand at 45.553 kb on + strand at 45.576 kb on + strand at 45.576 kb on + strand at 45.576 kb on + strand at 45.584 kb on - strand at 45.662 kb on + strand at 45.662 kb on + strand at 45.662 kb on + strand at 45.662 kb on + strand at 45.804 kb on + strand, within Atu3049 at 45.805 kb on - strand, within Atu3049 at 45.812 kb on - strand, within Atu3049 at 45.812 kb on - strand, within Atu3049 at 45.812 kb on - strand, within Atu3049 at 45.939 kb on - strand, within Atu3049 at 45.939 kb on - strand, within Atu3049 at 45.949 kb on - strand, within Atu3049 at 45.949 kb on - strand, within Atu3049 at 45.991 kb on + strand, within Atu3049 at 45.999 kb on - strand, within Atu3049 at 45.999 kb on - strand, within Atu3049 at 46.095 kb on - strand, within Atu3049 at 46.171 kb on - strand, within Atu3049 at 46.256 kb on - strand, within Atu3049 at 46.334 kb on - strand, within Atu3049 at 46.377 kb on - strand, within Atu3049 at 46.379 kb on + strand, within Atu3049 at 46.379 kb on + strand, within Atu3049 at 46.379 kb on + strand, within Atu3049 at 46.379 kb on + strand, within Atu3049 at 46.387 kb on - strand, within Atu3049 at 46.387 kb on - strand, within Atu3049 at 46.393 kb on + strand, within Atu3049 at 46.480 kb on - strand, within Atu3049 at 46.585 kb on - strand, within Atu3049 at 46.704 kb on - strand, within Atu3049 at 46.706 kb on + strand, within Atu3049 at 46.743 kb on + strand, within Atu3049 at 46.751 kb on - strand, within Atu3049 at 46.764 kb on - strand, within Atu3049 at 46.918 kb on - strand, within Atu3049 at 47.014 kb on + strand at 47.048 kb on - strand at 47.056 kb on - strand at 47.171 kb on + strand at 47.223 kb on - strand at 47.265 kb on - strand at 47.271 kb on + strand at 47.271 kb on + strand at 47.271 kb on + strand at 47.271 kb on + strand at 47.279 kb on - strand at 47.279 kb on - strand at 47.296 kb on - strand at 47.304 kb on + strand at 47.519 kb on + strand, within Atu3050 at 47.527 kb on - strand, within Atu3050 at 47.584 kb on + strand, within Atu3050 at 47.617 kb on + strand, within Atu3050 at 47.625 kb on - strand, within Atu3050 at 47.657 kb on - strand, within Atu3050 at 47.673 kb on - strand, within Atu3050 at 47.851 kb on + strand, within Atu3050 at 47.878 kb on + strand, within Atu3050 at 47.918 kb on + strand, within Atu3050 at 47.933 kb on - strand, within Atu3050 at 47.943 kb on - strand, within Atu3050 at 47.943 kb on - strand, within Atu3050 at 47.974 kb on + strand, within Atu3050 at 47.982 kb on - strand, within Atu3050 at 48.043 kb on - strand, within Atu3050 at 48.068 kb on + strand, within Atu3050 at 48.089 kb on + strand, within Atu3050 at 48.112 kb on + strand, within Atu3050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.02 mM remove 44,563 + Atu3048 0.15 -0.1 44,563 + Atu3048 0.15 -0.5 44,568 + Atu3048 0.16 -0.3 44,568 + Atu3048 0.16 -0.6 44,571 - Atu3048 0.16 -0.0 44,578 + Atu3048 0.17 -2.4 44,578 + Atu3048 0.17 -0.9 44,586 - Atu3048 0.18 -0.8 44,586 - Atu3048 0.18 -0.0 44,609 - Atu3048 0.20 +1.3 44,650 + Atu3048 0.24 -1.0 44,655 - Atu3048 0.25 -1.1 44,956 + Atu3048 0.56 -0.2 45,009 + Atu3048 0.62 -0.3 45,017 - Atu3048 0.63 -1.4 45,017 - Atu3048 0.63 +0.3 45,136 - Atu3048 0.75 -0.4 45,190 - Atu3048 0.81 +0.2 45,369 - -1.3 45,370 + -0.3 45,401 + -1.5 45,409 - +0.5 45,409 - -0.5 45,474 - -0.4 45,495 + -0.6 45,553 + -1.0 45,576 + -0.2 45,576 + -0.4 45,576 + -2.0 45,584 - +0.1 45,662 + +0.2 45,662 + -0.3 45,662 + +0.2 45,662 + -0.4 45,804 + Atu3049 0.18 -0.9 45,805 - Atu3049 0.18 -0.4 45,812 - Atu3049 0.19 -0.8 45,812 - Atu3049 0.19 -1.3 45,812 - Atu3049 0.19 -1.8 45,939 - Atu3049 0.26 -0.6 45,939 - Atu3049 0.26 -0.4 45,949 - Atu3049 0.27 -1.5 45,949 - Atu3049 0.27 +0.3 45,991 + Atu3049 0.30 +1.4 45,999 - Atu3049 0.30 +0.2 45,999 - Atu3049 0.30 +0.6 46,095 - Atu3049 0.36 +0.0 46,171 - Atu3049 0.40 -2.2 46,256 - Atu3049 0.46 +0.8 46,334 - Atu3049 0.50 -0.2 46,377 - Atu3049 0.53 -0.1 46,379 + Atu3049 0.53 +0.4 46,379 + Atu3049 0.53 +0.1 46,379 + Atu3049 0.53 -0.3 46,379 + Atu3049 0.53 -0.4 46,387 - Atu3049 0.53 -0.4 46,387 - Atu3049 0.53 -0.6 46,393 + Atu3049 0.54 -0.6 46,480 - Atu3049 0.59 +0.1 46,585 - Atu3049 0.65 +0.2 46,704 - Atu3049 0.73 +0.7 46,706 + Atu3049 0.73 +0.8 46,743 + Atu3049 0.75 -1.0 46,751 - Atu3049 0.75 +0.9 46,764 - Atu3049 0.76 -0.3 46,918 - Atu3049 0.85 +1.6 47,014 + -0.2 47,048 - +0.0 47,056 - +0.6 47,171 + -1.0 47,223 - -0.6 47,265 - +0.0 47,271 + -0.9 47,271 + -0.1 47,271 + +0.7 47,271 + -0.8 47,279 - -0.5 47,279 - +1.2 47,296 - +1.2 47,304 + -2.9 47,519 + Atu3050 0.17 -0.6 47,527 - Atu3050 0.17 -0.2 47,584 + Atu3050 0.22 -1.7 47,617 + Atu3050 0.24 -0.4 47,625 - Atu3050 0.25 +0.6 47,657 - Atu3050 0.27 -0.7 47,673 - Atu3050 0.28 -0.3 47,851 + Atu3050 0.42 -0.1 47,878 + Atu3050 0.44 +0.4 47,918 + Atu3050 0.47 -0.8 47,933 - Atu3050 0.48 -0.1 47,943 - Atu3050 0.48 -0.4 47,943 - Atu3050 0.48 +0.0 47,974 + Atu3050 0.51 +0.4 47,982 - Atu3050 0.51 -0.2 48,043 - Atu3050 0.56 +0.8 48,068 + Atu3050 0.58 -0.2 48,089 + Atu3050 0.59 -0.3 48,112 + Atu3050 0.61 -1.6
Or see this region's nucleotide sequence