Strain Fitness in Escherichia coli BW25113 around b3383

Experiment: limonene 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhfX and yhfY are separated by 83 nucleotidesyhfY and yhfZ are separated by 16 nucleotidesyhfZ and trpS are separated by 289 nucleotides b3381: yhfX - predicted amino acid racemase (NCBI), at 3,507,835 to 3,508,998 yhfX b3382: yhfY - orf, hypothetical protein (VIMSS), at 3,509,082 to 3,509,444 yhfY b3383: yhfZ - hypothetical protein (NCBI), at 3,509,461 to 3,510,366 yhfZ b3384: trpS - tryptophanyl-tRNA synthetase (NCBI), at 3,510,656 to 3,511,660 trpS Position (kb) 3509 3510 3511Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3508.486 kb on + strand, within yhfXat 3508.563 kb on + strand, within yhfXat 3508.567 kb on - strand, within yhfXat 3508.634 kb on + strand, within yhfXat 3508.634 kb on + strand, within yhfXat 3508.650 kb on - strand, within yhfXat 3508.652 kb on - strand, within yhfXat 3508.675 kb on - strand, within yhfXat 3508.675 kb on - strand, within yhfXat 3508.685 kb on + strand, within yhfXat 3508.823 kb on + strand, within yhfXat 3508.831 kb on - strand, within yhfXat 3508.833 kb on + strand, within yhfXat 3508.849 kb on - strand, within yhfXat 3508.849 kb on - strand, within yhfXat 3508.917 kb on + strandat 3508.950 kb on - strandat 3509.069 kb on + strandat 3509.069 kb on - strandat 3509.112 kb on - strandat 3509.215 kb on - strand, within yhfYat 3509.254 kb on + strand, within yhfYat 3509.254 kb on + strand, within yhfYat 3509.317 kb on - strand, within yhfYat 3509.349 kb on - strand, within yhfYat 3509.395 kb on + strand, within yhfYat 3509.395 kb on + strand, within yhfYat 3509.447 kb on - strandat 3509.502 kb on - strandat 3509.520 kb on + strandat 3509.524 kb on + strandat 3509.529 kb on - strandat 3509.529 kb on - strandat 3509.537 kb on - strandat 3509.539 kb on - strandat 3509.539 kb on - strandat 3509.547 kb on - strandat 3509.553 kb on - strand, within yhfZat 3509.572 kb on + strand, within yhfZat 3509.572 kb on + strand, within yhfZat 3509.576 kb on - strand, within yhfZat 3509.639 kb on - strand, within yhfZat 3509.723 kb on + strand, within yhfZat 3509.803 kb on + strand, within yhfZat 3509.803 kb on + strand, within yhfZat 3509.875 kb on - strand, within yhfZat 3509.888 kb on - strand, within yhfZat 3509.888 kb on - strand, within yhfZat 3509.926 kb on - strand, within yhfZat 3509.966 kb on + strand, within yhfZat 3509.966 kb on + strand, within yhfZat 3510.024 kb on - strand, within yhfZat 3510.058 kb on - strand, within yhfZat 3510.062 kb on + strand, within yhfZat 3510.084 kb on - strand, within yhfZat 3510.098 kb on - strand, within yhfZat 3510.098 kb on - strand, within yhfZat 3510.117 kb on - strand, within yhfZat 3510.124 kb on + strand, within yhfZat 3510.145 kb on - strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.584 kb on + strandat 3510.584 kb on + strandat 3510.596 kb on + strandat 3510.597 kb on + strandat 3510.602 kb on + strandat 3510.605 kb on + strandat 3510.628 kb on - strandat 3510.635 kb on + strandat 3510.635 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction limonene 5 vol%
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3,508,486 + yhfX b3381 0.56 +1.0
3,508,563 + yhfX b3381 0.63 -0.0
3,508,567 - yhfX b3381 0.63 +0.6
3,508,634 + yhfX b3381 0.69 -0.6
3,508,634 + yhfX b3381 0.69 +0.9
3,508,650 - yhfX b3381 0.70 -0.2
3,508,652 - yhfX b3381 0.70 +0.2
3,508,675 - yhfX b3381 0.72 +2.3
3,508,675 - yhfX b3381 0.72 +0.2
3,508,685 + yhfX b3381 0.73 +0.6
3,508,823 + yhfX b3381 0.85 -0.3
3,508,831 - yhfX b3381 0.86 +1.6
3,508,833 + yhfX b3381 0.86 -1.4
3,508,849 - yhfX b3381 0.87 +1.5
3,508,849 - yhfX b3381 0.87 +0.3
3,508,917 + -0.5
3,508,950 - -0.7
3,509,069 + +0.4
3,509,069 - -2.4
3,509,112 - +0.5
3,509,215 - yhfY b3382 0.37 +0.4
3,509,254 + yhfY b3382 0.47 +1.5
3,509,254 + yhfY b3382 0.47 -0.4
3,509,317 - yhfY b3382 0.65 +0.2
3,509,349 - yhfY b3382 0.74 -0.3
3,509,395 + yhfY b3382 0.86 -0.2
3,509,395 + yhfY b3382 0.86 -0.4
3,509,447 - +0.9
3,509,502 - +1.1
3,509,520 + -0.2
3,509,524 + -0.1
3,509,529 - +1.7
3,509,529 - +2.1
3,509,537 - +1.1
3,509,539 - +1.6
3,509,539 - +2.1
3,509,547 - +1.4
3,509,553 - yhfZ b3383 0.10 +1.8
3,509,572 + yhfZ b3383 0.12 +0.2
3,509,572 + yhfZ b3383 0.12 +0.4
3,509,576 - yhfZ b3383 0.13 +2.6
3,509,639 - yhfZ b3383 0.20 +1.3
3,509,723 + yhfZ b3383 0.29 -0.5
3,509,803 + yhfZ b3383 0.38 -0.5
3,509,803 + yhfZ b3383 0.38 +0.4
3,509,875 - yhfZ b3383 0.46 +0.8
3,509,888 - yhfZ b3383 0.47 +2.8
3,509,888 - yhfZ b3383 0.47 +1.8
3,509,926 - yhfZ b3383 0.51 +2.6
3,509,966 + yhfZ b3383 0.56 +0.6
3,509,966 + yhfZ b3383 0.56 +0.4
3,510,024 - yhfZ b3383 0.62 +2.4
3,510,058 - yhfZ b3383 0.66 +1.8
3,510,062 + yhfZ b3383 0.66 -0.7
3,510,084 - yhfZ b3383 0.69 +2.7
3,510,098 - yhfZ b3383 0.70 +1.1
3,510,098 - yhfZ b3383 0.70 +0.7
3,510,117 - yhfZ b3383 0.72 +0.5
3,510,124 + yhfZ b3383 0.73 +1.1
3,510,145 - yhfZ b3383 0.75 +2.6
3,510,189 + yhfZ b3383 0.80 +1.0
3,510,189 + yhfZ b3383 0.80 -0.3
3,510,189 + yhfZ b3383 0.80 +1.0
3,510,584 + +0.7
3,510,584 + +0.0
3,510,596 + +0.8
3,510,597 + +0.3
3,510,602 + +0.2
3,510,605 + +1.5
3,510,628 - +0.1
3,510,635 + -1.0
3,510,635 + +0.3

Or see this region's nucleotide sequence