Strain Fitness in Escherichia coli BL21 around ECD_01204

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01203 and ECD_01204 overlap by 4 nucleotidesECD_01204 and ECD_01205 overlap by 1 nucleotides ECD_01203: ECD_01203 - nitrate reductase 1, beta (Fe-S) subunit, at 1,271,752 to 1,273,290 _01203 ECD_01204: ECD_01204 - molybdenum-cofactor-assembly chaperone delta subunit of nitrate reductase 1, at 1,273,287 to 1,273,997 _01204 ECD_01205: ECD_01205 - nitrate reductase 1, gamma (cytochrome b(NR)) subunit, at 1,273,997 to 1,274,674 _01205 Position (kb) 1273 1274Strain fitness (log2 ratio) -1 0 1 2 3 4at 1272.287 kb on - strand, within ECD_01203at 1272.501 kb on + strand, within ECD_01203at 1272.549 kb on + strand, within ECD_01203at 1272.744 kb on + strand, within ECD_01203at 1272.916 kb on + strand, within ECD_01203at 1272.975 kb on + strand, within ECD_01203at 1273.470 kb on + strand, within ECD_01204at 1273.644 kb on + strand, within ECD_01204at 1274.084 kb on + strand, within ECD_01205at 1274.644 kb on + strandat 1274.644 kb on + strandat 1274.645 kb on - strandat 1274.645 kb on - strandat 1274.648 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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1,272,287 - ECD_01203 0.35 +1.4
1,272,501 + ECD_01203 0.49 +0.5
1,272,549 + ECD_01203 0.52 +0.2
1,272,744 + ECD_01203 0.64 +3.1
1,272,916 + ECD_01203 0.76 +0.2
1,272,975 + ECD_01203 0.79 -0.3
1,273,470 + ECD_01204 0.26 +4.7
1,273,644 + ECD_01204 0.50 +0.4
1,274,084 + ECD_01205 0.13 +1.7
1,274,644 + +2.2
1,274,644 + -0.3
1,274,645 - +0.3
1,274,645 - -0.1
1,274,648 + +1.1

Or see this region's nucleotide sequence