Strain Fitness in Escherichia coli BL21 around ECD_01131

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01129 and ECD_01130 are separated by 3 nucleotidesECD_01130 and ECD_01131 are separated by 35 nucleotidesECD_01131 and ECD_01132 are separated by 53 nucleotidesECD_01132 and ECD_01133 are separated by 9 nucleotides ECD_01129: ECD_01129 - adenylosuccinate lyase, at 1,193,983 to 1,195,353 _01129 ECD_01130: ECD_01130 - putative lysogenization regulator, at 1,195,357 to 1,195,998 _01130 ECD_01131: ECD_01131 - tRNA(Gln,Lys,Glu) U34 2-thiouridylase, at 1,196,034 to 1,197,140 _01131 ECD_01132: ECD_01132 - bifunctional thiamine pyrimidine pyrophosphate hydrolase and thiamine pyrophosphate hydrolase, at 1,197,194 to 1,197,655 _01132 ECD_01133: ECD_01133 - 23S rRNA pseudouridine(2457) synthase, at 1,197,665 to 1,198,318 _01133 Position (kb) 1196 1197 1198Strain fitness (log2 ratio) -1 0 1 2at 1195.529 kb on - strand, within ECD_01130at 1195.587 kb on - strand, within ECD_01130at 1195.587 kb on - strand, within ECD_01130at 1195.870 kb on - strand, within ECD_01130at 1195.870 kb on - strand, within ECD_01130at 1195.870 kb on - strand, within ECD_01130at 1195.870 kb on - strand, within ECD_01130at 1195.957 kb on - strandat 1196.019 kb on - strandat 1196.029 kb on - strandat 1196.029 kb on - strandat 1197.212 kb on - strandat 1197.224 kb on - strandat 1197.581 kb on - strand, within ECD_01132at 1197.715 kb on - strandat 1197.801 kb on + strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.802 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133at 1197.804 kb on - strand, within ECD_01133

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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1,195,529 - ECD_01130 0.27 +1.3
1,195,587 - ECD_01130 0.36 -0.4
1,195,587 - ECD_01130 0.36 -0.1
1,195,870 - ECD_01130 0.80 +0.7
1,195,870 - ECD_01130 0.80 +1.6
1,195,870 - ECD_01130 0.80 +0.5
1,195,870 - ECD_01130 0.80 +2.3
1,195,957 - -1.7
1,196,019 - +1.3
1,196,029 - +1.0
1,196,029 - +0.9
1,197,212 - +1.8
1,197,224 - +1.1
1,197,581 - ECD_01132 0.84 -0.9
1,197,715 - +0.3
1,197,801 + ECD_01133 0.21 +0.8
1,197,802 - ECD_01133 0.21 +0.3
1,197,802 - ECD_01133 0.21 +0.8
1,197,802 - ECD_01133 0.21 -1.1
1,197,804 - ECD_01133 0.21 +2.6
1,197,804 - ECD_01133 0.21 +0.3
1,197,804 - ECD_01133 0.21 +1.8
1,197,804 - ECD_01133 0.21 +1.4
1,197,804 - ECD_01133 0.21 -1.4
1,197,804 - ECD_01133 0.21 +2.0

Or see this region's nucleotide sequence