Strain Fitness in Escherichia coli BL21 around ECD_02422

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02419 and ECD_02420 are separated by 95 nucleotidesECD_02420 and ECD_02421 are separated by 16 nucleotidesECD_02421 and ECD_02422 are separated by 27 nucleotidesECD_02422 and ECD_02423 are separated by 111 nucleotides ECD_02419: ECD_02419 - HscA co-chaperone, J domain-containing protein Hsc56; IscU-specific chaperone HscAB, at 2,524,757 to 2,525,272 _02419 ECD_02420: ECD_02420 - FeS cluster assembly protein, at 2,525,368 to 2,525,691 _02420 ECD_02421: ECD_02421 - iron-sulfur cluster assembly scaffold protein, at 2,525,708 to 2,526,094 _02421 ECD_02422: ECD_02422 - cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent, at 2,526,122 to 2,527,336 _02422 ECD_02423: ECD_02423 - isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor, at 2,527,448 to 2,527,936 _02423 Position (kb) 2526 2527 2528Strain fitness (log2 ratio) -1 0 1at 2525.732 kb on - strandat 2525.732 kb on - strandat 2527.463 kb on - strandat 2527.508 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.701 kb on - strand, within ECD_02423at 2527.764 kb on - strand, within ECD_02423at 2527.816 kb on - strand, within ECD_02423at 2527.912 kb on - strandat 2527.942 kb on - strandat 2527.942 kb on - strandat 2527.942 kb on - strandat 2528.002 kb on - strandat 2528.068 kb on - strandat 2528.068 kb on - strandat 2528.071 kb on + strandat 2528.156 kb on - strandat 2528.156 kb on - strandat 2528.162 kb on + strandat 2528.163 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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2,525,732 - +0.4
2,525,732 - +0.1
2,527,463 - +0.7
2,527,508 - ECD_02423 0.12 +1.4
2,527,565 - ECD_02423 0.24 -0.1
2,527,565 - ECD_02423 0.24 +1.0
2,527,565 - ECD_02423 0.24 +0.9
2,527,565 - ECD_02423 0.24 +1.0
2,527,565 - ECD_02423 0.24 +0.8
2,527,701 - ECD_02423 0.52 +1.0
2,527,764 - ECD_02423 0.65 +1.7
2,527,816 - ECD_02423 0.75 +1.7
2,527,912 - +1.5
2,527,942 - +1.2
2,527,942 - +1.1
2,527,942 - +1.7
2,528,002 - +1.2
2,528,068 - +1.2
2,528,068 - -0.5
2,528,071 + +0.5
2,528,156 - -0.3
2,528,156 - +0.1
2,528,162 + +0.1
2,528,163 - +0.7

Or see this region's nucleotide sequence