Strain Fitness in Escherichia coli BL21 around ECD_01103

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01101 and ECD_01102 overlap by 20 nucleotidesECD_01102 and ECD_01103 are separated by 10 nucleotidesECD_01103 and ECD_01104 are separated by 22 nucleotidesECD_01104 and ECD_01105 are separated by 407 nucleotides ECD_01101: ECD_01101 - OM lipoprotein stimulator of MrcB transpeptidase, at 1,165,205 to 1,165,846 _01101 ECD_01102: ECD_01102 - thiamine kinase, at 1,165,827 to 1,166,651 _01102 ECD_01103: ECD_01103 - beta N-acetyl-glucosaminidase, at 1,166,662 to 1,167,687 _01103 ECD_01104: ECD_01104 - putative UPF0227 family esterase, at 1,167,710 to 1,168,252 _01104 ECD_01105: ECD_01105 - respiratory NADH dehydrogenase 2/cupric reductase, at 1,168,660 to 1,169,964 _01105 Position (kb) 1166 1167 1168Strain fitness (log2 ratio) -1 0 1 2at 1165.698 kb on + strand, within ECD_01101at 1165.734 kb on + strand, within ECD_01101at 1165.734 kb on + strand, within ECD_01101at 1165.913 kb on + strand, within ECD_01102at 1166.069 kb on - strand, within ECD_01102at 1166.160 kb on + strand, within ECD_01102at 1166.164 kb on + strand, within ECD_01102at 1166.272 kb on + strand, within ECD_01102at 1166.314 kb on + strand, within ECD_01102at 1166.327 kb on + strand, within ECD_01102at 1166.475 kb on + strand, within ECD_01102at 1166.509 kb on + strand, within ECD_01102at 1166.635 kb on + strandat 1166.638 kb on + strandat 1166.767 kb on + strand, within ECD_01103at 1167.241 kb on - strand, within ECD_01103at 1167.697 kb on - strandat 1167.795 kb on + strand, within ECD_01104at 1167.804 kb on + strand, within ECD_01104at 1168.417 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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1,165,698 + ECD_01101 0.77 +0.7
1,165,734 + ECD_01101 0.82 +1.1
1,165,734 + ECD_01101 0.82 -0.2
1,165,913 + ECD_01102 0.10 -0.2
1,166,069 - ECD_01102 0.29 -0.9
1,166,160 + ECD_01102 0.40 -1.3
1,166,164 + ECD_01102 0.41 +0.3
1,166,272 + ECD_01102 0.54 +1.7
1,166,314 + ECD_01102 0.59 +0.5
1,166,327 + ECD_01102 0.61 -0.9
1,166,475 + ECD_01102 0.79 -0.2
1,166,509 + ECD_01102 0.83 -0.2
1,166,635 + -0.1
1,166,638 + -0.4
1,166,767 + ECD_01103 0.10 -0.6
1,167,241 - ECD_01103 0.56 -1.3
1,167,697 - +2.7
1,167,795 + ECD_01104 0.16 -0.7
1,167,804 + ECD_01104 0.17 -1.3
1,168,417 - +2.0

Or see this region's nucleotide sequence