Strain Fitness in Escherichia coli BL21 around ECD_00201

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_t00003 and ECD_00200 are separated by 162 nucleotidesECD_00200 and ECD_00201 overlap by 4 nucleotidesECD_00201 and ECD_00202 are separated by 240 nucleotides ECD_t00003: ECD_t00003 - tRNA-Asp, at 231,855 to 231,931 _t00003 ECD_00200: ECD_00200 - 2,5-diketo-D-gluconate reductase B, at 232,094 to 232,897 _00200 ECD_00201: ECD_00201 - LysR family putative transcriptional regulator, at 232,894 to 233,808 _00201 ECD_00202: ECD_00202 - endo/exonuclease/phosphatase family protein, at 234,049 to 234,849 _00202 Position (kb) 232 233 234Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 231.934 kb on + strandat 231.934 kb on + strandat 231.937 kb on + strandat 232.362 kb on - strand, within ECD_00200at 232.424 kb on - strand, within ECD_00200at 232.566 kb on - strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.646 kb on - strand, within ECD_00200at 232.895 kb on + strandat 233.163 kb on - strand, within ECD_00201at 233.163 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.191 kb on - strand, within ECD_00201at 233.191 kb on - strand, within ECD_00201at 233.368 kb on - strand, within ECD_00201at 233.472 kb on + strand, within ECD_00201at 233.545 kb on - strand, within ECD_00201at 233.638 kb on - strand, within ECD_00201at 233.729 kb on - strandat 233.729 kb on - strandat 233.892 kb on + strandat 233.944 kb on + strandat 233.944 kb on + strandat 233.995 kb on + strandat 234.027 kb on - strandat 234.065 kb on - strandat 234.201 kb on - strand, within ECD_00202at 234.203 kb on - strand, within ECD_00202at 234.203 kb on - strand, within ECD_00202at 234.203 kb on - strand, within ECD_00202at 234.299 kb on + strand, within ECD_00202at 234.303 kb on - strand, within ECD_00202at 234.303 kb on - strand, within ECD_00202at 234.319 kb on - strand, within ECD_00202at 234.510 kb on + strand, within ECD_00202at 234.513 kb on - strand, within ECD_00202at 234.550 kb on - strand, within ECD_00202at 234.552 kb on + strand, within ECD_00202at 234.552 kb on + strand, within ECD_00202at 234.553 kb on - strand, within ECD_00202at 234.553 kb on - strand, within ECD_00202at 234.582 kb on - strand, within ECD_00202at 234.636 kb on + strand, within ECD_00202at 234.654 kb on - strand, within ECD_00202

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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231,934 + +1.5
231,934 + +1.6
231,937 + +1.7
232,362 - ECD_00200 0.33 -0.7
232,424 - ECD_00200 0.41 -2.1
232,566 - ECD_00200 0.59 +0.8
232,625 + ECD_00200 0.66 -1.0
232,625 + ECD_00200 0.66 -2.9
232,625 + ECD_00200 0.66 -0.3
232,626 - ECD_00200 0.66 -2.2
232,626 - ECD_00200 0.66 -1.6
232,646 - ECD_00200 0.69 -2.6
232,895 + -1.9
233,163 - ECD_00201 0.29 +0.1
233,163 - ECD_00201 0.29 -0.3
233,177 - ECD_00201 0.31 -1.1
233,177 - ECD_00201 0.31 -0.0
233,177 - ECD_00201 0.31 -0.1
233,177 - ECD_00201 0.31 +0.2
233,191 - ECD_00201 0.32 -1.5
233,191 - ECD_00201 0.32 -0.4
233,368 - ECD_00201 0.52 -0.8
233,472 + ECD_00201 0.63 -2.8
233,545 - ECD_00201 0.71 -0.2
233,638 - ECD_00201 0.81 -0.3
233,729 - +2.6
233,729 - -0.4
233,892 + -1.6
233,944 + -0.7
233,944 + -0.8
233,995 + -0.3
234,027 - -0.5
234,065 - -1.2
234,201 - ECD_00202 0.19 -0.7
234,203 - ECD_00202 0.19 -1.8
234,203 - ECD_00202 0.19 -1.2
234,203 - ECD_00202 0.19 -0.9
234,299 + ECD_00202 0.31 -1.9
234,303 - ECD_00202 0.32 -2.6
234,303 - ECD_00202 0.32 -1.0
234,319 - ECD_00202 0.34 -1.9
234,510 + ECD_00202 0.58 -1.1
234,513 - ECD_00202 0.58 -2.7
234,550 - ECD_00202 0.63 -1.3
234,552 + ECD_00202 0.63 -1.3
234,552 + ECD_00202 0.63 -1.8
234,553 - ECD_00202 0.63 -0.8
234,553 - ECD_00202 0.63 -0.9
234,582 - ECD_00202 0.67 -2.4
234,636 + ECD_00202 0.73 -1.1
234,654 - ECD_00202 0.76 -2.4

Or see this region's nucleotide sequence