Strain Fitness in Escherichia coli BW25113 around b1299

Experiment: pinene 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjL and puuR are separated by 26 nucleotidespuuR and puuC are separated by 274 nucleotides b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908 ycjL b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492 puuR b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254 puuC Position (kb) 1359 1360 1361Strain fitness (log2 ratio) -1 0 1at 1359.236 kb on + strand, within ycjLat 1359.236 kb on + strand, within ycjLat 1359.453 kb on + strand, within ycjLat 1359.594 kb on + strand, within ycjLat 1359.759 kb on - strand, within ycjLat 1360.081 kb on - strand, within puuRat 1360.084 kb on - strand, within puuRat 1360.317 kb on - strand, within puuRat 1360.332 kb on - strand, within puuRat 1360.334 kb on + strand, within puuRat 1360.413 kb on - strand, within puuRat 1360.478 kb on - strandat 1360.478 kb on - strandat 1360.521 kb on - strandat 1360.526 kb on - strandat 1360.526 kb on - strandat 1360.698 kb on - strandat 1360.698 kb on - strandat 1360.761 kb on - strandat 1360.903 kb on - strandat 1360.903 kb on - strandat 1360.933 kb on - strand, within puuCat 1361.146 kb on + strand, within puuCat 1361.146 kb on + strand, within puuCat 1361.262 kb on - strand, within puuC

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Per-strain Table

Position Strand Gene LocusTag Fraction pinene 5 vol%
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1,359,236 + ycjL b1298 0.12 +0.2
1,359,236 + ycjL b1298 0.12 +0.1
1,359,453 + ycjL b1298 0.40 +0.1
1,359,594 + ycjL b1298 0.59 -0.0
1,359,759 - ycjL b1298 0.80 +0.4
1,360,081 - puuR b1299 0.26 +0.2
1,360,084 - puuR b1299 0.27 -0.4
1,360,317 - puuR b1299 0.68 -0.1
1,360,332 - puuR b1299 0.71 -0.6
1,360,334 + puuR b1299 0.72 +0.1
1,360,413 - puuR b1299 0.86 -0.1
1,360,478 - +0.3
1,360,478 - +0.2
1,360,521 - -0.6
1,360,526 - +0.1
1,360,526 - +0.2
1,360,698 - +1.1
1,360,698 - +0.1
1,360,761 - +0.6
1,360,903 - -0.1
1,360,903 - +0.2
1,360,933 - puuC b1300 0.11 +0.1
1,361,146 + puuC b1300 0.25 -0.7
1,361,146 + puuC b1300 0.25 +0.9
1,361,262 - puuC b1300 0.33 +0.0

Or see this region's nucleotide sequence