Strain Fitness in Escherichia coli BL21 around ECD_02424

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02423 and ECD_02424 are separated by 451 nucleotidesECD_02424 and ECD_02425 are separated by 118 nucleotides ECD_02423: ECD_02423 - isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor, at 2,527,448 to 2,527,936 _02423 ECD_02424: ECD_02424 - tRNA mC32,mU32 2'-O-methyltransferase, SAM-dependent, at 2,528,388 to 2,529,128 _02424 ECD_02425: ECD_02425 - inositol monophosphatase, at 2,529,247 to 2,530,050 _02425 Position (kb) 2528 2529 2530Strain fitness (log2 ratio) -1 0 1at 2527.463 kb on - strandat 2527.508 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.565 kb on - strand, within ECD_02423at 2527.701 kb on - strand, within ECD_02423at 2527.764 kb on - strand, within ECD_02423at 2527.816 kb on - strand, within ECD_02423at 2527.912 kb on - strandat 2527.942 kb on - strandat 2527.942 kb on - strandat 2527.942 kb on - strandat 2528.002 kb on - strandat 2528.068 kb on - strandat 2528.068 kb on - strandat 2528.071 kb on + strandat 2528.156 kb on - strandat 2528.156 kb on - strandat 2528.162 kb on + strandat 2528.163 kb on - strandat 2528.402 kb on + strandat 2528.409 kb on - strandat 2528.424 kb on - strandat 2528.424 kb on - strandat 2528.570 kb on - strand, within ECD_02424at 2528.668 kb on + strand, within ECD_02424at 2528.811 kb on - strand, within ECD_02424at 2528.936 kb on + strand, within ECD_02424at 2529.163 kb on + strandat 2529.163 kb on + strandat 2530.028 kb on + strandat 2530.125 kb on - strandat 2530.125 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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2,527,463 - +0.9
2,527,508 - ECD_02423 0.12 +0.7
2,527,565 - ECD_02423 0.24 -0.5
2,527,565 - ECD_02423 0.24 +0.8
2,527,565 - ECD_02423 0.24 +1.2
2,527,565 - ECD_02423 0.24 +0.6
2,527,565 - ECD_02423 0.24 +0.5
2,527,701 - ECD_02423 0.52 -0.0
2,527,764 - ECD_02423 0.65 +1.2
2,527,816 - ECD_02423 0.75 +1.4
2,527,912 - +1.7
2,527,942 - +1.1
2,527,942 - +1.0
2,527,942 - +1.6
2,528,002 - +1.0
2,528,068 - +1.2
2,528,068 - -0.7
2,528,071 + +0.0
2,528,156 - -0.9
2,528,156 - -0.7
2,528,162 + +0.4
2,528,163 - -0.2
2,528,402 + -1.3
2,528,409 - +0.7
2,528,424 - +0.6
2,528,424 - +0.8
2,528,570 - ECD_02424 0.25 +0.1
2,528,668 + ECD_02424 0.38 +0.2
2,528,811 - ECD_02424 0.57 +0.9
2,528,936 + ECD_02424 0.74 +0.3
2,529,163 + -0.3
2,529,163 + -0.1
2,530,028 + +1.2
2,530,125 - -0.5
2,530,125 - -0.2

Or see this region's nucleotide sequence