Strain Fitness in Escherichia coli BL21 around ECD_01298

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01297 and ECD_01298 are separated by 155 nucleotidesECD_01298 and ECD_01299 overlap by 4 nucleotides ECD_01297: ECD_01297 - LacI family putative transcriptional repressor, at 1,369,330 to 1,370,328 _01297 ECD_01298: ECD_01298 - DUF463 family protein, putative P-loop NTPase, at 1,370,484 to 1,371,881 _01298 ECD_01299: ECD_01299 - UPF0283 family inner membrane protein, at 1,371,878 to 1,372,939 _01299 Position (kb) 1370 1371 1372Strain fitness (log2 ratio) -1 0 1 2 3at 1370.197 kb on + strand, within ECD_01297at 1370.328 kb on + strandat 1370.329 kb on - strandat 1370.439 kb on + strandat 1370.618 kb on + strandat 1370.647 kb on + strand, within ECD_01298at 1370.724 kb on + strand, within ECD_01298at 1370.746 kb on + strand, within ECD_01298at 1370.746 kb on + strand, within ECD_01298at 1370.748 kb on + strand, within ECD_01298at 1370.748 kb on + strand, within ECD_01298at 1370.748 kb on + strand, within ECD_01298at 1371.233 kb on + strand, within ECD_01298at 1371.478 kb on + strand, within ECD_01298at 1371.478 kb on + strand, within ECD_01298at 1371.527 kb on + strand, within ECD_01298at 1371.692 kb on + strand, within ECD_01298

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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1,370,197 + ECD_01297 0.87 -0.4
1,370,328 + -0.2
1,370,329 - -0.1
1,370,439 + +0.8
1,370,618 + +0.8
1,370,647 + ECD_01298 0.12 -0.1
1,370,724 + ECD_01298 0.17 +0.3
1,370,746 + ECD_01298 0.19 +1.9
1,370,746 + ECD_01298 0.19 +0.4
1,370,748 + ECD_01298 0.19 +1.1
1,370,748 + ECD_01298 0.19 +0.8
1,370,748 + ECD_01298 0.19 +0.7
1,371,233 + ECD_01298 0.54 -0.7
1,371,478 + ECD_01298 0.71 -1.6
1,371,478 + ECD_01298 0.71 +2.8
1,371,527 + ECD_01298 0.75 -0.4
1,371,692 + ECD_01298 0.86 +0.8

Or see this region's nucleotide sequence