Strain Fitness in Escherichia coli BL21 around ECD_01100

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01098 and ECD_01099 are separated by 333 nucleotidesECD_01099 and ECD_01100 are separated by 2 nucleotidesECD_01100 and ECD_01101 are separated by 13 nucleotidesECD_01101 and ECD_01102 overlap by 20 nucleotides ECD_01098: ECD_01098 - ferric-rhodotorulic acid outer membrane transporter, at 1,161,929 to 1,164,118 _01098 ECD_01099: ECD_01099 - purine nucleoside phosphoramidase, dadA activator protein, at 1,164,452 to 1,164,811 _01099 ECD_01100: ECD_01100 - uncharacterized protein, at 1,164,814 to 1,165,191 _01100 ECD_01101: ECD_01101 - OM lipoprotein stimulator of MrcB transpeptidase, at 1,165,205 to 1,165,846 _01101 ECD_01102: ECD_01102 - thiamine kinase, at 1,165,827 to 1,166,651 _01102 Position (kb) 1164 1165 1166Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1164.045 kb on - strandat 1164.181 kb on - strandat 1164.382 kb on + strandat 1164.387 kb on + strandat 1164.466 kb on + strandat 1164.568 kb on + strand, within ECD_01099at 1165.177 kb on + strandat 1165.182 kb on - strandat 1165.183 kb on + strandat 1165.698 kb on + strand, within ECD_01101at 1165.734 kb on + strand, within ECD_01101at 1165.734 kb on + strand, within ECD_01101at 1165.913 kb on + strand, within ECD_01102at 1166.069 kb on - strand, within ECD_01102at 1166.160 kb on + strand, within ECD_01102at 1166.164 kb on + strand, within ECD_01102

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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1,164,045 - +0.8
1,164,181 - +0.2
1,164,382 + -1.0
1,164,387 + -2.3
1,164,466 + -1.3
1,164,568 + ECD_01099 0.32 -1.3
1,165,177 + -0.1
1,165,182 - +3.7
1,165,183 + -1.8
1,165,698 + ECD_01101 0.77 -0.7
1,165,734 + ECD_01101 0.82 -0.6
1,165,734 + ECD_01101 0.82 -1.1
1,165,913 + ECD_01102 0.10 +0.5
1,166,069 - ECD_01102 0.29 -1.2
1,166,160 + ECD_01102 0.40 -0.1
1,166,164 + ECD_01102 0.41 +0.9

Or see this region's nucleotide sequence