Strain Fitness in Escherichia coli BL21 around ECD_00299

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00298 and ECD_00299 are separated by 122 nucleotidesECD_00299 and ECD_00300 are separated by 76 nucleotides ECD_00298: ECD_00298 - beta-D-galactosidase, at 332,766 to 335,840 _00298 ECD_00299: ECD_00299 - lactose-inducible lac operon transcriptional repressor, at 335,963 to 337,045 _00299 ECD_00300: ECD_00300 - mhp operon transcriptional activator, at 337,122 to 337,955 _00300 Position (kb) 335 336 337 338Strain fitness (log2 ratio) -1 0 1 2at 335.077 kb on - strand, within ECD_00298at 335.190 kb on - strand, within ECD_00298at 335.840 kb on + strandat 335.890 kb on - strandat 337.129 kb on - strandat 337.164 kb on - strandat 337.264 kb on + strand, within ECD_00300at 337.264 kb on + strand, within ECD_00300at 337.265 kb on - strand, within ECD_00300at 337.265 kb on - strand, within ECD_00300at 337.265 kb on - strand, within ECD_00300at 337.430 kb on - strand, within ECD_00300at 337.777 kb on + strand, within ECD_00300at 337.777 kb on + strand, within ECD_00300at 337.777 kb on + strand, within ECD_00300at 337.777 kb on + strand, within ECD_00300at 337.778 kb on - strand, within ECD_00300at 337.778 kb on - strand, within ECD_00300at 337.778 kb on - strand, within ECD_00300at 337.886 kb on + strandat 337.891 kb on - strandat 337.978 kb on - strandat 338.043 kb on + strandat 338.044 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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335,077 - ECD_00298 0.75 -0.5
335,190 - ECD_00298 0.79 -0.1
335,840 + -0.8
335,890 - +1.2
337,129 - -0.2
337,164 - -0.2
337,264 + ECD_00300 0.17 -1.3
337,264 + ECD_00300 0.17 -0.9
337,265 - ECD_00300 0.17 +0.0
337,265 - ECD_00300 0.17 +0.4
337,265 - ECD_00300 0.17 -0.0
337,430 - ECD_00300 0.37 +0.3
337,777 + ECD_00300 0.79 -0.6
337,777 + ECD_00300 0.79 +2.0
337,777 + ECD_00300 0.79 +0.8
337,777 + ECD_00300 0.79 -1.3
337,778 - ECD_00300 0.79 +0.8
337,778 - ECD_00300 0.79 +0.9
337,778 - ECD_00300 0.79 -0.9
337,886 + -1.3
337,891 - +0.7
337,978 - -0.6
338,043 + +0.6
338,044 - -0.5

Or see this region's nucleotide sequence