Strain Fitness in Escherichia coli BL21 around ECD_00028

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00025 and ECD_00026 are separated by 296 nucleotidesECD_00026 and ECD_00027 are separated by 21 nucleotidesECD_00027 and ECD_00028 are separated by 102 nucleotidesECD_00028 and ECD_00029 are separated by 7 nucleotidesECD_00029 and ECD_00030 are separated by 42 nucleotides ECD_00025: ECD_00025 - hypothetical protein, at 24,054 to 24,404 _00025 ECD_00026: ECD_00026 - hypothetical protein, at 24,701 to 24,865 _00026 ECD_00027: ECD_00027 - 30S ribosomal subunit protein S20, at 24,887 to 25,150 _00027 ECD_00028: ECD_00028 - uncharacterized protein, at 25,253 to 25,471 _00028 ECD_00029: ECD_00029 - bifunctional riboflavin kinase/FAD synthetase, at 25,479 to 26,420 _00029 ECD_00030: ECD_00030 - isoleucyl-tRNA synthetase, at 26,463 to 29,279 _00030 Position (kb) 25 26Strain fitness (log2 ratio) -1 0 1at 24.614 kb on + strandat 24.715 kb on + strandat 24.744 kb on + strand, within ECD_00026at 24.744 kb on + strand, within ECD_00026at 24.747 kb on + strand, within ECD_00026at 25.241 kb on + strandat 25.273 kb on + strandat 25.274 kb on - strandat 25.437 kb on + strand, within ECD_00028at 25.438 kb on - strand, within ECD_00028at 25.438 kb on - strand, within ECD_00028at 25.444 kb on + strand, within ECD_00028at 25.444 kb on + strand, within ECD_00028at 25.446 kb on + strand, within ECD_00028at 26.427 kb on + strandat 26.427 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_10
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24,614 + +0.6
24,715 + +0.0
24,744 + ECD_00026 0.26 +0.6
24,744 + ECD_00026 0.26 -0.6
24,747 + ECD_00026 0.28 -0.2
25,241 + +0.9
25,273 + +0.5
25,274 - +1.1
25,437 + ECD_00028 0.84 +0.1
25,438 - ECD_00028 0.84 -0.6
25,438 - ECD_00028 0.84 -0.4
25,444 + ECD_00028 0.87 +0.7
25,444 + ECD_00028 0.87 +1.0
25,446 + ECD_00028 0.88 +1.0
26,427 + +0.9
26,427 + +0.5

Or see this region's nucleotide sequence