Strain Fitness in Escherichia coli BL21 around ECD_02319

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02317 and ECD_02318 are separated by 42 nucleotidesECD_02318 and ECD_02319 are separated by 104 nucleotidesECD_02319 and ECD_02320 are separated by 32 nucleotidesECD_02320 and ECD_02321 are separated by 204 nucleotides ECD_02317: ECD_02317 - glucose-specific enzyme IIA component of PTS, at 2,408,221 to 2,408,730 _02317 ECD_02318: ECD_02318 - pyridoxal-pyridoxamine kinase/hydroxymethylpyrimidine kinase, at 2,408,773 to 2,409,624 _02318 ECD_02319: ECD_02319 - uncharacterized protein, at 2,409,729 to 2,410,103 _02319 ECD_02320: ECD_02320 - WGR domain protein, at 2,410,136 to 2,410,870 _02320 ECD_02321: ECD_02321 - cysteine synthase B (O-acetylserine sulfhydrolase B), at 2,411,075 to 2,411,986 _02321 Position (kb) 2409 2410 2411Strain fitness (log2 ratio) -2 -1 0 1at 2409.148 kb on - strand, within ECD_02318at 2409.339 kb on - strand, within ECD_02318at 2409.592 kb on - strandat 2409.723 kb on - strandat 2409.764 kb on - strandat 2409.882 kb on - strand, within ECD_02319at 2410.002 kb on + strand, within ECD_02319at 2410.002 kb on + strand, within ECD_02319at 2410.002 kb on + strand, within ECD_02319at 2410.002 kb on + strand, within ECD_02319at 2410.002 kb on + strand, within ECD_02319at 2410.004 kb on + strand, within ECD_02319at 2410.004 kb on + strand, within ECD_02319at 2410.004 kb on + strand, within ECD_02319at 2410.052 kb on - strand, within ECD_02319at 2410.060 kb on + strand, within ECD_02319at 2410.132 kb on + strandat 2410.204 kb on + strandat 2410.445 kb on + strand, within ECD_02320at 2410.446 kb on - strand, within ECD_02320at 2410.595 kb on + strand, within ECD_02320at 2410.644 kb on + strand, within ECD_02320at 2411.067 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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2,409,148 - ECD_02318 0.44 -2.2
2,409,339 - ECD_02318 0.66 -1.7
2,409,592 - -1.4
2,409,723 - -0.4
2,409,764 - +0.1
2,409,882 - ECD_02319 0.41 -1.1
2,410,002 + ECD_02319 0.73 -2.3
2,410,002 + ECD_02319 0.73 -1.4
2,410,002 + ECD_02319 0.73 -0.1
2,410,002 + ECD_02319 0.73 -1.0
2,410,002 + ECD_02319 0.73 -0.2
2,410,004 + ECD_02319 0.73 -1.0
2,410,004 + ECD_02319 0.73 -1.9
2,410,004 + ECD_02319 0.73 -1.1
2,410,052 - ECD_02319 0.86 -0.2
2,410,060 + ECD_02319 0.88 -0.0
2,410,132 + -0.0
2,410,204 + -0.6
2,410,445 + ECD_02320 0.42 -0.3
2,410,446 - ECD_02320 0.42 -0.9
2,410,595 + ECD_02320 0.62 -1.2
2,410,644 + ECD_02320 0.69 -1.4
2,411,067 - -1.1

Or see this region's nucleotide sequence